Abstract:
:Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylation proteins have yet to be performed. In this study, using LC-MS/MS qualitative proteomics strategies, we identified 1909 modification sites on 940 proteins in N. crassa and analysed the functions of these proteins using GO enrichment, KEGG pathway, and subcellular location experiments. We classified the acetylation protein involvement in diverse pathways, and protein-protein interaction (PPI) network analysis further demonstrated that these proteins participate in diverse biological processes. In summary, our study comprehensively profiles the crosstalk of modified sites, and PPI among these proteins may form a complex network with both similar and distinct regulatory mechanisms, providing improved understanding of their biological functions in N. crassa. This article is protected by copyright. All rights reserved.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Wang T,Guan W,Du Y,Xu Y,He Z,Zhang Y,Kang C,Wan X,Chi X,Sun K,Zhang Xdoi
10.1002/pmic.202000212subject
Has Abstractpub_date
2021-01-24 00:00:00pages
e2000212eissn
1615-9853issn
1615-9861pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Serine/threonine protein phosphatase 1 (PP1) regulates multiple cellular processes. Protein phosphorylation-dephosphorylation is largely altered during ischemia and subsequent reperfusion. The brain is particularly vulnerable to stress resulting from ischemia-reperfusion (IR), however, the acquisition of ischemic tole...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700214
更新日期:2007-09-01 00:00:00
abstract::Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500030
更新日期:2015-10-01 00:00:00
abstract::Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500278
更新日期:2006-03-01 00:00:00
abstract::The prediction of adverse drug reactions (ADRs) has become increasingly important, due to the rising concern on serious ADRs that can cause drugs to fail to reach or stay in the market. We proposed a framework for predicting ADR profiles by integrating protein-protein interaction (PPI) networks with drug structures. W...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200337
更新日期:2013-01-01 00:00:00
abstract::MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600682
更新日期:2007-02-01 00:00:00
abstract::We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500635
更新日期:2006-04-01 00:00:00
abstract::The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400072
更新日期:2014-10-01 00:00:00
abstract::Four rhoptry proteins (ROP) of Toxoplasma gondii previously identified with mAb have been affinity purified and analyzed by MS; the data obtained allowed the genomic sequences to be assigned to these proteins. As previously suggested for some of them by antibody crossreactivity, these proteins were shown to belong to ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600187
更新日期:2006-11-01 00:00:00
abstract::Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood or bile contaminatio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600086
更新日期:2007-04-01 00:00:00
abstract::Gene expression analyses of embryonic stem cells (ESCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal and pluripotency. We employed a 2-DE-based proteomics approach to analyze human ESC line, Royan H5, in undifferentiated cells and differ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900003
更新日期:2009-11-01 00:00:00
abstract::We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500511
更新日期:2006-03-01 00:00:00
abstract::Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900502
更新日期:2010-02-01 00:00:00
abstract::We have developed a proteome database (DB), BiomarkerDigger (http://biomarkerdigger.org) that automates data analysis, searching, and metadata-gathering function. The metadata-gathering function searches proteome DBs for protein-protein interaction, Gene Ontology, protein domain, Online Mendelian Inheritance in Man, a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800593
更新日期:2009-07-01 00:00:00
abstract::Unfortunately, conventional large-format polyacrylamide gels are mechanically fragile, often tearing during the subsequent manipulations required for visualization of the proteins. This problem is compounded when large-format two-dimensional gels are subjected to multiple staining procedures in order to detect differe...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300440
更新日期:2003-07-01 00:00:00
abstract::Lysine crotonylation (Kcr) is a recently discovered post-translational modification that potentially regulates multiple biological processes. With an objective to expand the available crotonylation datasets, LC-MS/MS is performed using mouse liver samples under normal physiological conditions to obtain in vivo crotony...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000049
更新日期:2020-10-01 00:00:00
abstract::Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800252
更新日期:2009-01-01 00:00:00
abstract::Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400442
更新日期:2015-05-01 00:00:00
abstract::The ability to obtain the accurate mass of a protein in a complex sample mixture aids in determining its correct in vivo form. This is important when identifying post-translationally modified proteins, protein variants or isoforms. The central technique used to separate proteins, 2-dimensional gel electrophoresis offe...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300843
更新日期:2004-10-01 00:00:00
abstract::As a consequence of their poor solubility during isoelectric focusing, integral membrane proteins are generally absent from two-dimensional gel proteome maps. In order to analyze the yeast plasma membrane proteome, a plasma membrane purification protocol was optimized in order to reduce contaminating membranes and cyt...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200212)2:12<1706::AID-PROT1706>3
更新日期:2002-12-01 00:00:00
abstract::Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400241
更新日期:2015-04-01 00:00:00
abstract::Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600654
更新日期:2007-06-01 00:00:00
abstract::A classical proteomic analysis was used to establish a reference map of proteins associated with healthy human erythrocyte ghosts. Following osmotic lysis and differential centrifugation, ghost proteins were separated by either one-dimensional gel electrophoresis (1-DE) or two-dimensional gel electrophoresis (2-DE). S...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200209)2:9<1229::AID-PROT1229>3.
更新日期:2002-09-01 00:00:00
abstract::O-GlcNAcylation is a dynamic PTM of nuclear and cytoplasmic proteins, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase, which catalyze the addition and removal of O-GlcNAc, respectively. This modification is associated with glucose metabolism, which plays important roles in many diseases including cancer. Altho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200126
更新日期:2013-07-01 00:00:00
abstract::Spatial organisation of proteins according to their function plays an important role in the specificity of their molecular interactions. Emerging proteomics methods seek to assign proteins to sub-cellular locations by partial separation of organelles and computational analysis of protein abundance distributions among ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000359
更新日期:2010-12-01 00:00:00
abstract::Adiponectin is a fat cell-secreted hormone with antidiabetic and anti-inflammatory activities. The reduced adiponectin levels are associated with obesity-related metabolic syndrome. Replenishment of this hormone into animal models can improve insulin sensitivity, decrease blood glucose and lipid levels, and prevent th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500840
更新日期:2006-07-01 00:00:00
abstract::The International Protein Index (IPI) database has been one of the most widely used protein databases in MS proteomics approaches. Recently, the closure of IPI in September 2011 was announced. Its recommended replacement is the new UniProt Knowledgebase (UniProtKB) "complete proteome" sets, launched in May 2011. Here,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100363
更新日期:2011-11-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900025
更新日期:2019-11-01 00:00:00
abstract::Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs)...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800423
更新日期:2019-12-01 00:00:00
abstract::An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-conce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600184
更新日期:2007-07-01 00:00:00
abstract::Human butyrophilin (BTN) expression in milk fat globule (MFGM) was evaluated using two dimensional electrophoresis (2-DE) as the separation technique, and peptide mass mapping by matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) as the identification tool. Since milk composition changes througho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200207)2:7<850::AID-PROT850>3.0.
更新日期:2002-07-01 00:00:00