Abstract:
:The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertility, embryo development and heredity. The recent developments in MS have boosted the potential for identification and study of the sperm proteins. Catalogues of hundreds to thousands of spermatozoan proteins in human and in model species are becoming available setting up the basis for subsequent research, diagnostic applications and the development of specific treatments. The present article reviews the available scientific publications dealing with the composition and function of the sperm cell using an MS proteomic approach.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Oliva R,de Mateo S,Estanyol JMdoi
10.1002/pmic.200800588subject
Has Abstractpub_date
2009-02-01 00:00:00pages
1004-17issue
4eissn
1615-9853issn
1615-9861journal_volume
9pub_type
杂志文章,评审相关文献
PROTEOMICS文献大全abstract::Basal-like breast cancers are commonly negative for expression of estrogen and progesterone receptors and HER-2 (triple-negative breast cancer), which makes this subtype of breast cancers more aggressive and less responsive to standard treatment. We have applied a small-scale chemical proteomics method using bisindoly...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000801
更新日期:2011-07-01 00:00:00
abstract::Eukaryotic lipid rafts are membrane microdomains that have significant amounts of cholesterol and a selective set of proteins that have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol o...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500093
更新日期:2015-11-01 00:00:00
abstract::In proteomic research, two-dimensional electrophoresis (2-D) is an important tool for investigating differential patterns of qualitative and quantitative protein expression. The strength of the technique is due to its unrivalled power of being able to separate simultaneously thousands of proteins. The key to the compa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200107)1:7<856::AID-PROT856>3.0.
更新日期:2001-07-01 00:00:00
abstract::The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900409
更新日期:2020-11-01 00:00:00
abstract::Leukaemic transformation is frequently associated with the aberrant activity of a protein tyrosine kinase (PTK). As such it is of clinical relevance to be able to map the effects of these leukaemogenic PTKs on haemopoietic cells at the level of phosphorylation modulation. In this paradigm study we have employed a rang...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000176
更新日期:2010-09-01 00:00:00
abstract::Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100436
更新日期:2012-04-01 00:00:00
abstract::In this study, poly(glycidyl methacrylate-ethyleneglycol dimethacrylate) monolith functionalized with cobalt phthalocyanine tetracarboxylic acid is prepared. The polymer monolithic material is used for glycopeptides enrichment coupled with MALDI-TOF MS. By taking advantage of cobalt phthalocyanine including hydrogen b...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700399
更新日期:2018-10-01 00:00:00
abstract::Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800166
更新日期:2019-09-01 00:00:00
abstract::Long-term storage of protein samples for transportation is a challenge in the field of mass spectrometric analysis because the low temperature condition is not easy to be maintained. Here we introduce a simple method to preserve proteins at room temperature for at least one month. In this method, the protein sample is...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700336
更新日期:2018-07-01 00:00:00
abstract::The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201700223
更新日期:2019-05-01 00:00:00
abstract::Cyanobacteria are oxygenic photosynthetic prokaryotes and play a crucial role in the Earth's carbon and nitrogen cycles. The photoautotrophic cyanobacterium Anabaena sp. PCC 7120 has the ability to fix atmospheric nitrogen in heterocysts and produce hydrogen as a byproduct through a nitrogenase. In order to improve hy...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800332
更新日期:2019-10-01 00:00:00
abstract::Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this study, the complementar...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800200
更新日期:2018-12-01 00:00:00
abstract::Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800266
更新日期:2018-09-01 00:00:00
abstract::Antibiotics disturb the physiological homeostasis of bacterial cells by interfering with essential cellular functions or structures. The bacterial proteome adjusts quickly in response to antibiotic challenge. This physiological response is specifically tailored to overcome the inflicted damage and, thus, closely linke...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100046
更新日期:2011-08-01 00:00:00
abstract::The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400072
更新日期:2014-10-01 00:00:00
abstract::Proteome analysis by two-dimensional gel electrophoresis (2-DE) together with mass spectrometry was applied to screen acute phase response (APR)-related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300864
更新日期:2004-12-01 00:00:00
abstract::Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at di...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701079
更新日期:2009-02-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs. A large-scale outbreak of PED occurred in China in 2010, with PEDV emerging in the United States in 2013 and spreading rapidly, posing significant economic and p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400458
更新日期:2015-06-01 00:00:00
abstract::2-D analysis of plant proteomes containing thousands of proteins has limited dynamic resolution because only abundant proteins can be detected. Proteomic assessment of the non-abundant proteins within seeds is difficult when 60-80% is storage proteins. Resolution can be improved through sample fractionation using sepa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800875
更新日期:2009-06-01 00:00:00
abstract::Chemokines (CCs) are small chemoattractant cytokines involved in a wide variety of biological and pathological processes. Released by cells in the milieu, and extracellular matrix and activating signalling cascades upon binding to specific G protein-coupled receptors (GPCRs), they trigger many cellular events. In vari...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800211
更新日期:2008-11-01 00:00:00
abstract::Most proteins execute their functions through interacting with other proteins. Thus, understanding protein-protein interactions (PPIs) is essential to decipher biological functions in a living cell. To predict large-scale PPIs, effective and efficient computational approaches are desirable to integrate heterogeneous d...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200326
更新日期:2013-01-01 00:00:00
abstract::Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800397
更新日期:2009-05-01 00:00:00
abstract::Separation and identification of hydrophobic membrane proteins is a major challenge in proteomics. Identification of such sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins by peptide mass fingerprinting (PMF) via matrix-assisted laser desorption/ionization-time of flight (MALDI-TO...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400851
更新日期:2004-12-01 00:00:00
abstract::The protein components of urine are useful indicators of renal function and human health in general. Urine samples are easily attainable making them ideal substrates for biomarker research. Analysis of the urine proteome however, has been hindered by the great variability of the urine specimens, and the presence of va...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500671
更新日期:2006-08-01 00:00:00
abstract::The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis proce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100313
更新日期:2012-01-01 00:00:00
abstract::Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401037
更新日期:2005-04-01 00:00:00
abstract::Carcinoma of the prostate (CaP) is the second leading cause of cancer-related mortality among American men. While high cure rates are associated with localized CaP, no cure exists for advanced recurrent disease. At present there are no known serologic biomarkers specific to this stage of the disease. Several groups ha...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401165
更新日期:2005-07-01 00:00:00
abstract::Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500129
更新日期:2006-03-01 00:00:00
abstract::Myostatin plays a major role in muscle growth and development and animals with disruption of this gene display marked increases in muscle mass. Little is known about muscle physiological adaptations in relation to this muscle hypertrophy. To provide a more comprehensive view, we analyzed bovine muscles from control, h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400925
更新日期:2005-02-01 00:00:00
abstract::The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200401015
更新日期:2005-02-01 00:00:00