Abstract:
:RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock-down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interference (RNAi) transfection reagents, DharmaFECT 1 and INTERFERin, in mock transfections using non-targeting siRNAs, cause alterations in the lipidome of HeLa cells. Some lipids change in response to both, presumably chemically different, transfection reagents, while other lipid species change only in response to one of the reagents. While the functional implications of these lipidomic alterations remain to be investigated, the authors' experiments suggest that it is important to use appropriate mock transfection controls during RNAi experiments, ideally complemented by an orthogonal perturbation, especially when investigating membrane-associated phenomena.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Özbalci C,Storck EM,Eggert USdoi
10.1002/pmic.201800298subject
Has Abstractpub_date
2019-07-01 00:00:00pages
e1800298issue
13eissn
1615-9853issn
1615-9861journal_volume
19pub_type
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