Abstract:
:Calsyntenin-2 (Clstn2) and calsyntenin-3 (Clstn3) are the members of the cadherin superfamily and function to regulate the postsynaptic activity. Both proteins are known to play an important role in memory and learning. This study was designed to test the hypothesis that exposure of mothers to Pb in drinking water may alter the expression of Clstn2 and Clstn3 in offspring, which contributes to the Pb-induced learning deficiency. Pregnant mice were exposed to Pb in drinking water as Pb acetate from gestation to weaning. At the postnatal day 21, the learning and memory ability of pups was tested by Morris water maze, and the blood and brain tissues from pups were collected for metal and protein analyses. Data showed that perinatal Pb exposure resulted in a dose-dependent increase of Pb concentrations in blood (6-20-fold), hippocampus (2-7-fold), and cerebral cortex (2-8-fold) in offspring, as compared to controls (p < 0.05).The ability of learning and memory was decreased in lead exposure group, as compared to controls (p < 0.05). Both immunofluorescence and Western blot analyses revealed a striking difference in the expression of Clstn2 vs. Clstn3 following perinatal Pb exposure. In pregnant mice exposed to 0.1%, 0.2%, and 0.5% Pb, the expression of Clstn2 in offspring showed a Pb dose-related decrease by 39.2%, 76.5%, and 96.1% in hippocampus and by12.5%, 59.4%, and 78.1% in cerebral cortex, respectively (p < 0.05). In contrast, Clstn3 expression in these offspring brain regions was significantly increased (p < 0.05), after perinatal Pb exposure. The nature of Pb differential effect on Clstn2 and Clstn3 remains unknown. These observations suggest that Clstn2 and Clstn3 may have different roles in synaptic development and differentiation. Pb-induced learning defects may partly relate to the altered expression of calsyntenin proteins.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Li N,Cao S,Yu Z,Qiao M,Cheng Y,Shen Y,Song L,Huang X,Yang G,Zhao Ydoi
10.1007/s12011-020-02241-5subject
Has Abstractpub_date
2020-06-16 00:00:00eissn
0163-4984issn
1559-0720pii
10.1007/s12011-020-02241-5pub_type
杂志文章abstract::The degree of chromosome damage induced by three compounds of zinc (zinc chloride, zinc sulfate, zinc acetate) was compared in human leucocytes in vitro. Three concentrations of each salt, 3.0 x 10(-5)M, 3.0 x 10(-4)M, and 1.5 x 10(-3)M, were added to leukocyte cultures. The cells were harvested after 48 and 72 h and ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:78:1-3:113
更新日期:2000-01-01 00:00:00
abstract:UNLABELLED:There is conflicting evidence regarding the relationship between magnesium deficiency and metabolic syndrome, and a systematic assessment of the literature has not been performed. Our objective was to clarify the association between magnesium levels and metabolic syndrome by performing a meta-analysis. Based...
journal_title:Biological trace element research
pub_type: 杂志文章,meta分析
doi:10.1007/s12011-015-0446-9
更新日期:2016-03-01 00:00:00
abstract::Selenium (Se) is a trace element for human and animal health. Cadmium (Cd) is a known human carcinogen. The effects of Cd on the environment and humans are well known. Because chickens are at the top of the food chain, it is a good experimental animal model for assessing heavy metal toxicity and its potential threat t...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-019-01858-5
更新日期:2020-06-01 00:00:00
abstract::The present study was conducted to assess the influence of dietary zinc nanoparticles (size 50 nm) on the growth, biochemical constituents, enzymatic antioxidant levels and the nonspecific immune response of the freshwater prawn, Macrobrachium rosenbergii post larvae (PL). The concentrations of dietary supplement zinc...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-0026-4
更新日期:2014-07-01 00:00:00
abstract::This study examined the effects of dietary (2.0 ppm for 8 wk) and in vitro (1 x 10(-7)M) supplementation with selenium (Se, as sodium selenite) on the activity of spleen natural killer (NK) cells and plastic-adherent lymphokine-activated killer (A-LAK) cells from C57B1/6J male mice. Dietary supplementation with Se res...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02789164
更新日期:1996-06-01 00:00:00
abstract::For this study, we investigate more deeply the effect calcium (Ca) develops on the mechanism underlying fluoride-triggered osteocyte apoptosis. We detected the morphology of osteocytes by HE staining, mitochondrial microstructure by using the transmission electron microscope, and the biochemical indexes related to bon...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02305-6
更新日期:2020-07-25 00:00:00
abstract::Quercetin, a plant-derived flavonoid, is frequently used as an antioxidant for efficient anti-oxidative capacity. However, whether quercetin has protective effects on goat sperm and preimplantation embryos against Cd2+-induced oxidative injury is still unclear. So, we researched the influence of quercetin on goat sper...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1255-8
更新日期:2018-10-01 00:00:00
abstract::A survey based on 838 samples of milk obtained from 537 dairies covering 70 of 95 districts in France was organized to assess iodine content of milk and its contribution to total intake. Iodine levels were significantly higher in winter than in summer. Very low iodine contents (less than 25 micrograms I/kg) were found...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784607
更新日期:1992-01-01 00:00:00
abstract::Six important metal contents (i.e., zinc, barium, magnesium, calcium, copper, and selenium) in blood samples coupled with an ensemble classification algorithm have been used for the classification of normal people and cancer patients. A dataset containing 42 healthy samples and 32 cancer samples was used for experimen...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-010-8864-1
更新日期:2011-10-01 00:00:00
abstract::The present study investigated the role of kisspeptin-10 on reproductively significant trace elements in relation to testosterone release in male rabbits, Oryctolagus cuniculus. Groups of rabbits were exposed to single 1 μg kisspeptin dose (i.v., saphenous vein), while simultaneous groups were pretreated with a kisspe...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9720-x
更新日期:2013-08-01 00:00:00
abstract::The developmental alterations in metallothionein (MT) proteins and zinc (Zn) were investigated in brains of two transgenic strains of mice. MT protein was measured by a cadmium binding assay and Zn by atomic absorption spectrophotometry. MT proteins were expressed at birth (day 1) both in MT-I overexpressing transgeni...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784039
更新日期:1998-01-01 00:00:00
abstract::The present study was undertaken to investigate the utility of cystatin C (CysC) as an early biomarker of cadmium (Cd)-induced renal injury. The study was carried out on 50 adult male individuals divided into five groups of 10 individuals as follows: control, welders, smoker welders, diabetic welders, and smoker diabe...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-9939-1
更新日期:2014-06-01 00:00:00
abstract::In order to study the metabolism of mercury (Hg), its affinity to metallothionein (MT), and its influence on levels of the essential metals copper and zinc in the brain tissue of rats exposed to elemental mercury (HgO) vapor was investigated. The major findings were: 1. After long-term exposure, about 40% of mercury w...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02789402
更新日期:1993-04-01 00:00:00
abstract::Hypomagnesemia is associated with the development of neuropathy and abnormal platelet activity, both of which are risk factors for diabetic foot ulcer (DFU). This study was carried out to evaluate the effects of magnesium administration on wound healing and metabolic status in subjects with DFU. This randomized, doubl...
journal_title:Biological trace element research
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12011-017-1056-5
更新日期:2018-02-01 00:00:00
abstract::The objective of this study was to determine the effects of supplemental chromium as chromium picolinate (CrPic) on productive performance, chromium (Cr) concentration, serum parameters, and colostrum composition in sows. Thirty Yorkshire sows were bred with semen from a pool of Landrace boars. The sows were equally g...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9699-3
更新日期:2013-07-01 00:00:00
abstract::The present study was aimed at estimating the current (2012) dietary intake of arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) by the population of Catalonia, Spain. The temporal trends with respect to previous surveys, performed in 2000, 2005, and 2008, were also determined. For that purpose, metal concentrat...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-0138-x
更新日期:2014-12-01 00:00:00
abstract::In this study, we evaluated the synergistic effects of genistein and zinc on bone metabolism, bone mineral density, and the femoral metaphyseal histomorphology in the ovariectomized rats. Sixty female Sprague-Dawley rats, aged 13 weeks, were divided into sham-operated group (SHAM), ovariectomized group (OVX), genistei...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-017-1134-8
更新日期:2018-06-01 00:00:00
abstract::The objective of this study was to investigate the iron source absorption in the small intestine of broiler. In situ ligated intestinal loops of 70 birds were poured into one of seven solutions, including inorganic iron (FeSO4, Fe2(SO4)3), organic Fe glycine chelate (Fe-Gly(II), Fe-Gly(III)), the mixtures (FeSO4 with ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-0179-1
更新日期:2015-02-01 00:00:00
abstract::It was determined if the sensitivity in macular mutant mouse to copper-induced toxicity was affected by sex or age. The sensitivity in 6-8-d-old or 3-4-wk-old macular mutant mouse to copper-induced toxicity was not affected by sex. However, 8-9-wk-old mutant females were more sensitive to copper-induced toxicity than ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783183
更新日期:1993-11-01 00:00:00
abstract::The authors present a simple and rapid method for assaying ultrafilterable copper (Cu UF) and albumin-bound copper (Cu ALB). It is based on the ultrafiltration of serum in the presence of ethylenediaminetetraacetic acid (EDTA), used to prevent adsorption on the membranes. EDTA at 0.4 g/L has no effect on the equilibri...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02917499
更新日期:1988-12-01 00:00:00
abstract::Garlic (Allium sativum) is known to reduce lead toxicity in some species of animals. The objective of this study was to evaluate the efficacy of allicin, one of the most active components of garlic, in the treatment of subacute lead intoxication in sheep. Nine female sheep weighing 25-29 kg orally received a daily dos...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-008-8185-9
更新日期:2008-01-01 00:00:00
abstract::Over the last decade, there has been an increased concern about the health risks from exposure to arsenic at low doses, because of their neurotoxic effects on the developing brain. The exact mechanism underlying arsenic-induced neurotoxicity during sensitive periods of brain development remains unclear, although enhan...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1286-1
更新日期:2018-11-01 00:00:00
abstract::Electron energy loss spectroscopy (EELS) is a high spatial resolution electron microscopic technique with the potential to quantify elements at the subcellular level. The presence of each element is demonstrated by the electron energy loss edge at the energy characteristic of that element. The area of the edge may ind...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02916819
更新日期:1994-01-01 00:00:00
abstract::A working analytical procedure for selenium determinations by INAA, using gamma spectrometry with a germanium well-type detector, is presented. The spectral line at 400.7 keV is employed, enhanced with the effect of energy summing in true coincidence of cascade photons. The main advantage of the method is high efficie...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02917304
更新日期:1994-10-01 00:00:00
abstract::Extracellular Ca2+-influx induced by trivalent metal ions (Fe3+, Al3+, Cr3+, In3+, Ga3+, and La3+) in Ehrlich carcinoma cells is enhanced by ATP. This action seems to be related to the high coordination capacity of the ATP ligand that inhibits the polymerization of the solvated cations taking place at physiological pH...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02820027
更新日期:1998-04-01 00:00:00
abstract::Zinc has been reported to enhance the response to interferon (IFN) or PEG-IFN plus ribavirin therapy, improve liver function, and ameliorate hematologic side effects in patients with chronic hepatitis C. However, the role of zinc supplementation during IFN therapy in chronic hepatitis B infection (CHB) remains unclear...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-011-8971-7
更新日期:2011-12-01 00:00:00
abstract::The effect ofin utero zinc deficiency on fetal development in rats is reviewed. Attention is paid to the primary biochemical lesion associated with zinc-related teratogenesis and special consideration is given to the central nervous system. Evidence is presented that the thymidine kinase salvage pathway, used for the ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02916569
更新日期:1985-03-01 00:00:00
abstract::Aspergillus terreus was cultivated on Harrold's medium supplemented with 0.1% (w/v) cadmium chloride as well as on sulfur free medium amended with 0.1% (w/v) sodium selenite and potassium tellurite separately. The cell free extract of the fungus for each treatment was fractionated on a column packed with Sephadex G 75...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02990464
更新日期:1991-03-01 00:00:00
abstract::The onset of Alzheimer's disease has been shown to affect trace element concentrations in the brain when compared to "normal" subjects in ex vivo samples. The techniques used to determine trace element concentrations were proton-induced X-ray emission and instrumental neutron activation analysis. With these methods, s...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784242
更新日期:1999-01-01 00:00:00
abstract::A simple, eco-friendly, green routine co-precipitation method was experimented to synthesize iron nanoparticles (Fe-NPs) using the cell-free supernatant of actinobacteria. The biosynthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffractometer (XRD), energy-dispersive X-ray (EDX), scanning ele...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-019-01777-5
更新日期:2020-03-01 00:00:00