Simple assay of serum copper fractions by ultrafiltration and flameless atomic absorption.

Abstract:

:The authors present a simple and rapid method for assaying ultrafilterable copper (Cu UF) and albumin-bound copper (Cu ALB). It is based on the ultrafiltration of serum in the presence of ethylenediaminetetraacetic acid (EDTA), used to prevent adsorption on the membranes. EDTA at 0.4 g/L has no effect on the equilibrium of serum copper vectors and enables Cu UF to be assayed by flameless atomic absorption. EDTA at 2 g/L is used to assay total exchangeable copper (CU EXC) (ultrafilterable + albumin-bound). The evaluation criteria of the method are furnished, as are the normal values in healthy subjects: 14.6 micrograms/L for Cu UF and 87 micrograms/L for Cu EXC. Finally, the usefulness of the methods described here for the diagnosis of Wilson's and Menkes' disease was demonstrated.

journal_name

Biol Trace Elem Res

authors

Favier A,Ruffieux D

doi

10.1007/BF02917499

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

145-60

eissn

0163-4984

issn

1559-0720

journal_volume

18

pub_type

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