Abstract:
:For this study, we investigate more deeply the effect calcium (Ca) develops on the mechanism underlying fluoride-triggered osteocyte apoptosis. We detected the morphology of osteocytes by HE staining, mitochondrial microstructure by using the transmission electron microscope, and the biochemical indexes related to bone metabolism and the expression of apoptosis-related genes. These results showed that NaF brought out the reduced osteocytes and ruptured mitochondrial outer membrane, with a significantly increased StrACP activity by 10.414 IU/L at the 4th week (P < 0.05), markedly upregulating the mRNA expression of Bax, Cyto-C, Apaf-1, caspase-7, ROCK-1, BMP-2, and BGP (P < 0.01), as well as caspase-6 (P < 0.05), while downregulating Bcl-2 by 61.3% (P < 0.01). Through immunohistochemical analysis, we also found that NaF notably increased the protein expression of ROCK-1 (P < 0.05) and Cyto-C, BMP-2, and BGP (P < 0.01), suggesting that NaF triggered the activation of the mitochondrial apoptotic pathway and Rho/ROCK signaling pathway. Nevertheless, 1% Ca supplementation in diet notably enhanced the mRNA expression of Bcl-2 by 39.3% (P < 0.01), thus blocking the increment of the expression of mitochondrial apoptotic pathway-related genes and ROCK-1. Meanwhile, Ca could attenuate the StrACP activity by 10.741 IU/L at the 4th week (P < 0.05) and protect the integrity of the mitochondrial outer membrane. These findings strongly suggest that 1% Ca abated the mitochondrial apoptosis pathway by increasing the anti-apoptotic gene Bcl-2 expression, and effectively inhibited the hyper-activation of ROCK-1, dually protecting the structural integrity of the mitochondrial outer membrane and maintaining normal cellular metabolic function.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Yang J,Zhu Y,Zhang D,Yan Z,Zhao Y,Manthari RK,Cheng X,Wang J,Wang Jdoi
10.1007/s12011-020-02305-6subject
Has Abstractpub_date
2020-07-25 00:00:00eissn
0163-4984issn
1559-0720pii
10.1007/s12011-020-02305-6pub_type
杂志文章abstract::The aim of this work was to explore the effects of a low- and high-zinc diet and vitamin Aon the distribution of fatty acids in chylomicrons. Mongolian Gerbils were fed a basal diet (for 3 wk) containing 8 or 38 mg zinc/kg of feed (low-zinc group [termed LZ group] and saturated zinc group [termed SZ group], respective...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:112:2:145
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journal_title:Biological trace element research
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journal_title:Biological trace element research
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783810
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journal_title:Biological trace element research
pub_type: 杂志文章,meta分析
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783177
更新日期:1993-02-01 00:00:00
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journal_title:Biological trace element research
pub_type: 临床试验,杂志文章
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更新日期:2017-02-01 00:00:00
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journal_title:Biological trace element research
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-021-02604-6
更新日期:2021-01-26 00:00:00
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journal_title:Biological trace element research
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
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journal_title:Biological trace element research
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
pub_type: 杂志文章
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abstract::Numerous experiments in vitro and in vivo have shown that an appropriate increase intake of silicon can facilitate the synthesis of collagen and its stabilization and promote the differentiation and mineralization of osteoblasts. In this study, we examined whether ortho-silicic acid restrains the differentiation of os...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02286-6
更新日期:2020-07-16 00:00:00
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journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783830
更新日期:1999-12-01 00:00:00
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journal_title:Biological trace element research
pub_type: 杂志文章
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journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9640-9
更新日期:2013-06-01 00:00:00
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journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783258
更新日期:1982-06-01 00:00:00
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journal_title:Biological trace element research
pub_type: 临床试验,杂志文章
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