Abstract：
:Metallothionein (MT) is a low-mol-wt protein. The essential trace elements copper and zinc and the potentially toxic elements, such as cadmium, can induce the synthesis of and bind to MT. The major functions of MT are related to metal metabolism. This paper reported and evaluated a new method for determination of hepatic and renal MT of rat by using high performance liquid chromatography (HPLC) with a Superdex 75 gel filtration column. The tissue was homogenized and centrifuged, then the supernatant was pretreated simply by cadmium saturation and heating before HPLC determination. The MT was completely and clearly separated from other proteins in the rat tissues in a short time, and was quantitated directly as a function of UV absorbance at 250 nm. The recovery both for hepatic and renal MT of rat were exceed 90%. The coefficient of variation was 1.3% for hepatic MT of rat and 1.7% for renal MT of rat. The detection limit was 0.265 microgram for hepatic MT and 0.095 microgram for renal MT of rat. The present method was compared with the traditional cadmium-saturation method for determination of hepatic and renal MT of rat. A good correlation was found in these two methods.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Jin N,Kimura M,Yokoi K,Itokawa Ydoi
10.1007/BF02783177subject
Has Abstractpub_date
1993-02-01 00:00:00pages
183-90issue
2eissn
0163-4984issn
1559-0720journal_volume
36pub_type
杂志文章abstract：:Nickel is an important kind of metal and a necessary trace element in people's production and livelihood; it is also a well-confirmed human carcinogen. In the past few years, researchers did a large number of studies about the molecular mechanisms of nickel carcinogenesis, and they focused on activation of proto-oncog...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-013-9619-6
更新日期：2013-05-01 00:00:00
abstract：:The present work aimed at assessing passive, innate, and acquired immunity in piglets from sows supplemented with either organic or inorganic selenium (Se). A total of 12 multiparous pregnant sows were randomly allocated to three groups: selenium-deficient, corn and soy-based diet base diet (BD), 0.3 mg Se/kg as hydro...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-019-01754-y
更新日期：2020-03-01 00:00:00
abstract：:Extracellular Ca2+-influx induced by trivalent metal ions (Fe3+, Al3+, Cr3+, In3+, Ga3+, and La3+) in Ehrlich carcinoma cells is enhanced by ATP. This action seems to be related to the high coordination capacity of the ATP ligand that inhibits the polymerization of the solvated cations taking place at physiological pH...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02820027
更新日期：1998-04-01 00:00:00
abstract：:Conflicting results exist between selenium concentration and preeclampsia. The role of selenium in the development of preeclampsia is unclear. We conducted a meta-analysis to compare the blood selenium level in patients with preeclampsia and healthy pregnant women, and to determine the effectiveness of selenium supple...
journal_title：Biological trace element research
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s12011-015-0545-7
更新日期：2016-06-01 00:00:00
abstract：:The aim of the present study was to investigate the effect of adding selenium (Se) in cereal production by fertilization on Se concentration in laying hen eggs. Tritordeum (×Tritordeum Ascherson et Graebner), a new cereal from the cross between durum wheat and a wild barley species having accreditation as natural crop...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-016-0652-0
更新日期：2016-09-01 00:00:00
abstract：:Copper (Cu) parenteral administration is used in a beef cow-calf operations to prevent or correct Cu deficiency in bovines. At present, Zinc (Zn) salts have been incorporated to complement Cu antioxidant effect. A risk of hepatotoxicity generated by overdose is a negative consequence of injectable Cu application. Cu-Z...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-017-0965-7
更新日期：2017-10-01 00:00:00
abstract：:Plasma zinc (Zn) concentrations were measured in 4376 indigent women (86% African-American), at at mean (+/- SD) gestational age of 15 (+/- 7.8) wk to determine the relationship between various maternal characteristics and plasma Zn levels during pregnancy. Mean Plasma An levels were lower in African-American women th...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02784174
更新日期：1996-10-01 00:00:00
abstract：:Sponge granuloma formation was compared in copper-deficient and copper-sufficient rats following feeding of respective diets for 20, 40, or 60 d. Body weight, total blood hemoglobin, and activities of ceruloplasmin and Cu, Zn-superoxide dismutase in plasma were monitored to ascertain copper deficiency. Mean granuloma ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02990272
更新日期：1990-05-01 00:00:00
abstract：:In vitro tests simulating the elements release from inhaled urban particulate matter (PM) with artificial lung fluids (Gamble's and Hatch's solutions) and simulated gastric and pancreatic solutions were applied for an estimation of hazardous element (As, Cd, Cr, Hg, Mn, Ni, Pb and Zn) bio-accessibility in this materia...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-0101-x
更新日期：2014-11-01 00:00:00
abstract：:A survey of mercury (Hg) and selenium (Se) contents was performed in fish collected from lakes located in two National Parks of the northern patagonian Andean range. Two native species, catfish (Diplomystes viedmensis) and creole perch (Percichthys trucha), and three introduced species, brown trout (Salmo trutta), rai...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-007-8059-6
更新日期：2008-04-01 00:00:00
abstract：:The effect of zinc deficiency on calmodulin function was investigated by assessing the in vivo activity of two calmodulin regulated enzymes, adenosine 3',5'-monophosphate (c-AMP) and guanosine 3',5'-monophosphate (c-GMP) phosphodiesterase (PDE) in several rat tissues. Enzymatic activities in brain, heart, and testis o...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02797137
更新日期：1988-08-01 00:00:00
abstract：:Serum iron levels have been shown to decline both with fever and with strenuous exercise, leading to the supposition that the decrease might be the result of a rise in core body temperature. To evaluate this hypothesis, the serum iron response to an exercise-induced 1.5 degrees C rise in core body temperature was meas...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:108:1-3:061
更新日期：2005-01-01 00:00:00
abstract：:This study concerns application of flame atomic absorption spectrometry (FAAS) in assessment of macro- and microelement and toxic metal levels (Mg, Ca, K, Na, Mn, Cu, Fe, Zn, Cr, Ni, Co, Cd and Pb) in dark (Pu-erh) and fruit tea leaves and their infusions. Phosphorus was also determined in the form of phosphomolybdate...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-016-0669-4
更新日期：2016-11-01 00:00:00
abstract：:The contribution of gamma-ray scattering to the total efficiency for point-source geometries in a gamma spectrometer was measured. Scattering of gamma-rays on the shield and in the sample was considered. For a 25% efficiency detector in a small shield, about a 1% contribution to the total efficiency was measured, wher...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02917374
更新日期：1994-10-01 00:00:00
abstract：:Calcium, magnesium, and zinc balances were determined in eleven young adult college females (mean age, 24.9±2.35) during a 39-d metabolic study when the subjects were fed an adequate calorie and protein diet based on habitually consumed foods. Analysis showed that the dietary contribution of calcium, magnesium, and zi...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02917454
更新日期：1985-11-01 00:00:00
abstract：:Two 42-d experiments were conducted with weanling male rats to study interactions between nickel and copper. In Experiment 1, a low-copper basal diet was supplemented with copper at 0 or 30 ppm and nickel at 0 or 30 ppm. Copper was added in Experiment 2 to a basal copper-deficient diet at a level of 0 or 15 ppm and ni...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02916540
更新日期：1985-04-01 00:00:00
abstract：:Strontium stimulates cartilage matrix formation in vitro. However, the mechanisms governing these effects have not yet been extensively reported. In this study, chondrocytes were isolated from rat articular cartilage by enzymatic digestion and cultured for 24-72 h with 1-5 mM strontium. We investigated the effects of ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-013-9640-9
更新日期：2013-06-01 00:00:00
abstract：:Studies have evidenced that zinc metabolism is altered in presence of Down syndrome, and zinc seems to have a relationship with the metabolic alterations usually present in this syndrome. In this work, the Zn-related nutritional status of adolescents with Down syndrome was evaluated by means of biochemical parameters ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-007-0061-5
更新日期：2007-01-01 00:00:00
abstract：:After considering the need for quality control in NAA, the concept of quality in NAA procedures themselves is discussed, and some important factors identified. Two approaches to improve quality are then described in more detail. The first concerns the unique ability of NAA using different isotopic reactions and differ...
journal_title：Biological trace element research
pub_type： 杂志文章,评审
doi：10.1007/BF02917356
更新日期：1994-10-01 00:00:00
abstract：:Iron deficiency anemia (IDA) due to malnutrition and/or blood loss is a common condition, especially in women of reproductive age. Intense exercise can induce anemia via an inflammatory response, but whether intense exercise affects the efficacy of iron supplementation to treat IDA is unclear. Here, we show in a mouse...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-020-02402-6
更新日期：2020-10-06 00:00:00
abstract：:Plasma zinc (Zn), copper (Cu), and magnesium (Mg) concentrations, copper/zinc ratio, and selenium (Se) status were studied in 44 vegetarians (22 males and 22 females) and their age- and sex-matched nonvegetarians in the Bratislava region (Slovakia). Vegetarians had statistically significant lower levels of plasma Zn a...
journal_title：Biological trace element research
pub_type： 临床试验,杂志文章
doi：10.1007/BF02789145
更新日期：1995-10-01 00:00:00
abstract：:Deficiency of intracellular magnesium (icMg) may coexist with normal serum Mg levels. Little is known about clinical and pharmacological factors affecting icMg in normomagnesemic patients with diabetes mellitus (DM). Moreover, no information exists regarding the icMg state in diabetic patients after acute illness and ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-007-8024-4
更新日期：2007-01-01 00:00:00
abstract：:This study was performed to determine the effects of selenium supplementation on clinical symptoms and gene expression related to inflammatory markers in infertile women with polycystic ovary syndrome (PCOS) who were candidate for in vitro fertilization (IVF). Thirty-six women candidate for IVF were recruited in this ...
journal_title：Biological trace element research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s12011-019-01715-5
更新日期：2020-02-01 00:00:00
abstract：:This study was performed to investigate calcium (Ca), inorganic phosphorus (P), potassium (K), magnesium (Mg), and chlorine (Cl) levels in blood serum at d 60, 100, and 150 of gestation and at d 45 after parturition and to find out the significance of differences for macromineral levels during these stages of single o...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:107:3:247
更新日期：2005-12-01 00:00:00
abstract：:The relationship between selenium (Se) deficiency-induced cardiac malfunction and endoplasmic reticulum (ER) stress is poorly understood. In the present study, 18 weaning Sprague Dawley rats were randomly fed with three different Se diets, and myocardial glutathione peroxidase (GPx) activity was measured by an enzyme ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-013-9834-1
更新日期：2013-12-01 00:00:00
abstract：:Heavy metals toxicity is a prevalent health problem particularly in developing countries. Mercury and cadmium are toxic elements that have no physiologic functions in human body. They should not be present in the human body by any concentration. Copper, on the other hand, is one of the elements that are essential for ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-015-0525-y
更新日期：2016-06-01 00:00:00
abstract：:Present study investigates relationships between total and bioaccessibility of trace elements (Cd, Co, Cr, Cu, Mn, NI, Pb, V, and Zn) concentrations in sediment and their bioaccumulation in species in Shadegan wetland in southwest of Iran. Bioavailability factor (BAF) and translocation factor (TF) were calculated in p...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-010-8795-x
更新日期：2011-09-01 00:00:00
abstract：:The glutathione transferase (GST) activity of rat liver cytosolic preparations with ethacrynic acid (EA) and (+/-)-7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydro-benzo(a)pyrene (BPDE) as substrates, increased by 125 and 350%, respectively, in animals that had been treated with a single intraveno...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02917265
更新日期：1989-07-01 00:00:00
abstract：:Multivariate statistical analysis has been applied to time series measurements of aerosol elemental composition from PIXE analysis of filter samples, and principal components have been resolved that represent distinct particle types in an external mixture in the atmosphere. In this study, it is argued that a combinati...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02992673
更新日期：1990-07-01 00:00:00
abstract：:The toxic effect of Pb2+ has been studied in eukaryotic cells by using Tetrahymena as a target. The maximum power (P(m)) and the growth rate constant (k) were determined, which showed that values of P(m) and k were linked to the concentration (C) of Pb2+. The addition of Pb2+ caused a decrease of the maximum heat prod...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-007-0055-3
更新日期：2007-11-01 00:00:00
