Abstract：
:Strontium stimulates cartilage matrix formation in vitro. However, the mechanisms governing these effects have not yet been extensively reported. In this study, chondrocytes were isolated from rat articular cartilage by enzymatic digestion and cultured for 24-72 h with 1-5 mM strontium. We investigated the effects of different concentrations of strontium on collagen content, type II collagen, insulin-like growth factor (IGF-1) and matrix metalloproteinase (MMP)-13 expression in rat cultured articular chondrocytes in vitro. The collagen content of the chondrocytes, determined as hydroxyproline, was measured by a colorimetry method. Type II collagen, IGF-1, and MMP-13 mRNA abundance and protein expression levels were determined by real-time polymerase chain reaction (real-time PCR) and western blot, respectively. The results showed that collagen content from the chondrocytes extracellular matrix increased with increasing strontium concentration. Moreover, 3 and 5 mM strontium strongly stimulated protein expression and mRNA levels of type II collagen and IGF-1. Conversely, MMP-13 expression in chondrocytes decreased dose-dependently with increasing strontium concentration. These results should provide insight into the ability of strontium to promote chondrocyte extracellular matrix synthesis. Strontium could promote collagen synthesis and suppress collagen degradation via the repression of MMP-13 expression.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Wang J,Zhu X,Liu L,Shi X,Yin L,Zhang Y,Li X,Wang Z,Liu Gdoi
10.1007/s12011-013-9640-9subject
Has Abstractpub_date
2013-06-01 00:00:00pages
212-9issue
1-3eissn
0163-4984issn
1559-0720journal_volume
153pub_type
杂志文章abstract：:Concentrations of major nutritional and trace elements (Ca, P, Mg, Na, K, Fe, Zn, Cu, Mn, Se, Co, Cr, Ni, Sr, and Ba), as well as toxic heavy metals (Cd and Pb), were analyzed in the longissimus muscle of Charolais, Hereford, and Simmental bulls. The elements were determined using inductively coupled plasma optical em...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-9913-y
更新日期：2014-04-01 00:00:00
abstract：:Experiments were conducted to determine whether the increased glutathione S-transferase (GSH-T) activity associated with selenium (Se) deficiency is necessarily related to losses in the activity of Se-dependent glutathione peroxidase (SeGSHpx) in chicks. Nutritional Se status was altered in two ways: by treatment with...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02783792
更新日期：1993-05-01 00:00:00
abstract：:Deficiency in the intake of trace elements, such as copper (Cu), iron (Fe), selenium (Se), and zinc (Zn), is very common in the general population of most developing countries. A preliminary study in India and Pakistan showing the plasma levels of Zn and Fe indicates that approx 50% of the subjects who participated ha...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02789092
更新日期：1998-03-01 00:00:00
abstract：:The present study was conducted to investigate the effects of chromium histidinate (CrHis) against experimentally induced type II diabetes and on chromium (Cr), zinc (Zn), selenium (Se), manganese (Mn), iron (Fe), and copper (Cu) in serum, liver, and kidney of diabetic rats. The male Wistar rats (n = 60, 8 weeks old) ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-009-8351-8
更新日期：2009-11-01 00:00:00
abstract：:Thirty rats were divided randomly into five groups and fed with different feeds. They were killed after 2 mo. Right thighs of these rats were taken as samples. Bone mineral densities of these samples were measured by the double-energy x-ray bone mineral densitometer, and trace elements contents of the samples were ana...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02784252
更新日期：1999-01-01 00:00:00
abstract：:Studies investigating the effects of Chromium (Cr) supplementation on unstressed broiler chickens have reported an inconsistent association between Cr supplementation and broiler chickens' responses. We conducted a dose-response meta-analysis of previous studies to assess the dose-response associations between Cr supp...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-021-02589-2
更新日期：2021-01-17 00:00:00
abstract：:The study was undertaken to investigate the influence of alpha-tocopherol on zinc, copper, iron, calcium, magnesium, and potassium concentrations in serum of rats with bleomycin-induced pulmonary fibrosis. Fourteen Wistar albino rats were randomly divided into two groups of seven animals each. The first group was trea...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:114:1:175
更新日期：2006-01-01 00:00:00
abstract：:The aim of the present study was to investigate the effect of adding selenium (Se) in cereal production by fertilization on Se concentration in laying hen eggs. Tritordeum (×Tritordeum Ascherson et Graebner), a new cereal from the cross between durum wheat and a wild barley species having accreditation as natural crop...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-016-0652-0
更新日期：2016-09-01 00:00:00
abstract：:Abnormal placentation is the likely cause of the slow fetal growth and the high levels of circulating lipid peroxides found in severe pre-eclampsia. These peroxides are probably responsible for the high thromboxane:prostacyclin ratio found in this disease and may participate in the endothelial cell damage which is its...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02784164
更新日期：1996-10-01 00:00:00
abstract：:The objective of the study was analysis of hair trace elements content in children with goiter living in Aktubinsk region. Children with goiter and age- and sex-adjusted controls were involved in the current study. Hair trace elements content was assessed using inductively coupled plasma mass spectrometry. Thyroid vol...
journal_title：Biological trace element research
pub_type： 临床试验,杂志文章
doi：10.1007/s12011-016-0711-6
更新日期：2016-12-01 00:00:00
abstract：:The aim of the present study was to examine the effects of exogenous selenium (Se) supplementation on the tolerance of pepper (Capsicum annuum L.) cv. Suryamukhi Cluster plants to cadmium (Cd) phytotoxicity at the reproductive stage. The pepper plants were supplied with Cd (0, 0.25 or 0.50 mM) and Se (0, 3 or 7 μM), i...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-0028-2
更新日期：2014-07-01 00:00:00
abstract：:Molybdenum (Mo) is an essential element for human beings and animals; however, high dietary intake of Mo can lead to adverse reactions. Cadmium (Cd) is harmful to health. To investigate the toxicity of Mo combined with Cd in duck kidneys, 240 ducks were randomly divided into six groups and treated with a commercial di...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-015-0348-x
更新日期：2015-11-01 00:00:00
abstract：:The current information about the effect of physical exercise on the body concentrations of several minerals is still limited, both in the acute (short-term) and adaptive (long-term) responses. So, this manuscript aims, on the one hand, to assess the possible differences on basal levels of cobalt (Co), copper (Cu), an...
journal_title：Biological trace element research
pub_type： 临床试验,杂志文章
doi：10.1007/s12011-018-1500-1
更新日期：2019-06-01 00:00:00
abstract：:The humoral immune response was evaluated in male CD-1 mice fed the iron deficient (7 ppm Fe), iron sufficient (120 ppm Fe), and high-iron diets (3000 or 5000 ppm Fe) for 54 d. The IgM and IgG antibody responses against sheep erythrocytes (SRBC) determined by hemolytic plaque assay were suppressed by 65.4 and 51.2%, r...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02790075
更新日期：1994-10-01 00:00:00
abstract：:Multielemental detection using Instrumental Neutron Activation Analysis (INAA) and Proton-Induced X-Ray Emission (PIXE) of drinkable water of Florence (Italy) is described. The concentrations of 52 elements were measured. No chemical treatment was performed on the samples, before or after irradiation. On the basis of ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02992691
更新日期：1990-07-01 00:00:00
abstract：:Variations in trace element concentration in the head and facial hair of five individuals with ever-increasing amounts of white hair were examined. Hair was collected from the scalp, cheeks, and chin from one donor on a regular basis since 1984. Samples were separated into white and pigmented fractions, and analyzed a...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02992728
更新日期：1990-07-01 00:00:00
abstract：:To elucidate the element content of newborn blood vessels, umbilical arteries and veins in human umbilical cords, which had the advantage of easy sampling, were examined by ICP-AES. Umbilical cords were removed after birth. Mothers' ages ranged from 26 to 35 yr. It was found that the content of sulfur was the highest ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02783875
更新日期：1999-09-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake. The name of "Ali Ghorbani Ranjbary" is now corrected in the author group of this article. The original article has been corrected. ...
journal_title：Biological trace element research
pub_type： 杂志文章,已发布勘误
doi：10.1007/s12011-019-01787-3
更新日期：2020-04-01 00:00:00
abstract：:Silicon (Si) is important for the growth and development of bone and connective tissues. Several studies have reported that Si supplementation improved bone mineral density (BMD) in female ovarectomized rats. However, few studies have investigated the effects of Si supplementation on bone status and bone metabolism in...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-9936-4
更新日期：2014-05-01 00:00:00
abstract：:Serum selenium levels were determined cross-sectionally in 57 HIV-infected patients who were classified according to the Centers for Disease Control (CDC) 1993 classification system. Mean serum selenium levels were lower in CDC stage II (58.7 +/- 12.2 micrograms/L; p < 0.01; n = 18) and stage III (47.6 +/- 11.3 microg...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02778982
更新日期：1997-01-01 00:00:00
abstract：:The contribution of gamma-ray scattering to the total efficiency for point-source geometries in a gamma spectrometer was measured. Scattering of gamma-rays on the shield and in the sample was considered. For a 25% efficiency detector in a small shield, about a 1% contribution to the total efficiency was measured, wher...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02917374
更新日期：1994-10-01 00:00:00
abstract：:Cantharellus cibarius Fr. (chanterelle) and Boletus badius Pers. (bay bolete) harvested from natural sites in Poland were used to derive in vitro cultures. The optimal medium composition for cultures was developed. Concentrations of the chosen elements (Zn, Cu, Fe, Mg, Ni, and Cd) in mycelium samples were measured by ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-013-9670-3
更新日期：2013-06-01 00:00:00
abstract：:Selenium is a trace element that, although toxic in higher concentrations, is essential for human and animal health. In this study, we looked at microarray-based gene expression patterns from liver and gastrocnemius tissues in mice fed either a selenium-deficient diet or diets containing sodium selenite, selenomethion...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-010-8894-8
更新日期：2011-11-01 00:00:00
abstract：:Silver nanoparticles (AgNPs) have recently emerged as a powerful agents for disinfection in the poultry industry. AgNPs are capable of epithelial barriers passing from the route of exposure to the vital organs and cells. This study evaluated the effects of AgNPs on organs weights, blood biochemical, hematological, and...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-018-1267-4
更新日期：2018-10-01 00:00:00
abstract：:A double label procedure with 57Co and 58Co has been developed for detailed in vivo studies of erythrocyte survival. A well-type Ge detector is used in the measurements. The activities necessary for these experiments are very low, and the associated dose received by the test persons can be neglected. ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02992664
更新日期：1990-07-01 00:00:00
abstract：:A cohort study on platers in Tokyo was carried out using the records of the Tokyo Health Insurance Society of Plating Industry. The type of work and chromium-exposure history of each member of this society was investigated by a questionnaire sent to the manager of each factory. The recovery rate of the questionnaire w...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02783841
更新日期：1979-03-01 00:00:00
abstract：:In this study, eight kinds of nickel (Ni) compounds were orally administered to Wistar male rats and the distribution of each compound was investigated 24 h after the administration. The Ni compounds used in this experiment were nickel metal [Ni-M], nickel oxide (green) [NiO(G)], nickel oxide (black) [NiO(B)], nickel ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02789001
更新日期：1995-07-01 00:00:00
abstract：:Assessment of trace element levels in general population from the specific area is of importance for nutritional and occupational monitoring. In the current study, baseline blood levels of 12 toxic and/or essential metals and metalloids, including arsenic (As), cadmium (Cd), lead (Pb), mercury (Hg), chromium (Cr), tha...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-018-1486-8
更新日期：2019-06-01 00:00:00
abstract：:An in vitro system employing collagen isolated from the sheep tendons to induce mineralization and demineralization reactions was used not only to study the effect of various concentrations of fluoride on the collagen-induced mineralization and demineralization reactions but also to compare their action with the inhib...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-020-02340-3
更新日期：2020-08-27 00:00:00
abstract：:This experiment was conducted to evaluate the effects of heme and non-heme iron sources on growth performances, blood parameters, tissue mineral concentration, and intestinal morphology in weanling pigs. At 25 days of age, 32 newly weaned piglets (Duroc × Landrace × Yorkshire; 8.66 ± 0.59 kg) were allocated to one of ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-018-1385-z
更新日期：2019-02-01 00:00:00
