The effect of copper on glutathione metabolism in human leukocytes.

Abstract:

:Leukocytes incubated with Cu(II) showed a decrease in both glutathione reductase activity and reduced glutathione content. The glucose 6-phosphate dehydrogenase activity under the same conditions was not affected. Serum albumin added to mixtures prevented the loss of enzyme activity, whileD-penicillamine andL-histidine had little effect. Prior oxidation of the cell-reduced glutathione did not diminish the enzyme inhibitory action of Cu(II). The amount of regeneration of reduced glutathione in leukocytes previously treated with diamide to oxidize their reduced glutathione was a function of Cu(II) concentration in the media. No evidence was obtained that elevated serum ceruloplasmin levels in rabbits, nor incubation of leukocytes in vitro with ceruloplasmin, affect leukocyte glutathione reductase activity. It was proposed that the major mechanism by which copper affects glutathione metabolism in leukocytes is by inhibition of glutathione reductase.

journal_name

Biol Trace Elem Res

authors

Rafter GW

doi

10.1007/BF02783258

subject

Has Abstract

pub_date

1982-06-01 00:00:00

pages

191-7

issue

2-3

eissn

0163-4984

issn

1559-0720

journal_volume

4

pub_type

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