Abstract:
BACKGROUND:Diagnosis of eosinophilic esophagitis (EoE) requires manual quantification of tissue eosinophils. Eosinophil peroxidase (EPX) is an eosinophil-specific, cytoplasmic granule protein released during degranulation. AIMS:The objective of this study was to evaluate image analysis of EPX immunohistochemistry as an automated method for histologic diagnosis of EoE. METHODS:We performed a secondary analysis of prospectively collected esophageal biopsies obtained from adult subjects with EoE and controls. Tissue sections were stained with hematoxylin and eosin (H&E) and evaluated for peak eosinophils per high power field (eos/hpf). The same slides were de-stained and re-stained to detect EPX for direct comparison. Slides were digitized, and EPX staining area/mm2 was quantified using image analysis. Paired samples were compared for changes in EPX staining in treatment responders and non-responders. RESULTS:Thirty-eight EoE cases and 49 controls were analyzed. Among EoE subjects, matched post-treatment biopsies were available for 21 responders and 10 non-responders. Baseline EPX/mm2 was significantly increased in EoE subjects and decreased in treatment responders. EPX quantification correlated strongly with eos/hpf (r = 0.84, p < 0.0001) and identified EoE subjects with high diagnostic accuracy (AUC 0.95, p < 0.0001). The optimal diagnostic EPX-positive pixel/area threshold was 17,379 EPX/mm2. Several controls (5/49) with < 15 eos/hpf on H&E staining exceeded this cutoff. CONCLUSIONS:EPX/mm2 correlates strongly with eos/hpf, accurately identifies subjects with EoE, and decreases in treatment responders. Automated quantification of intact eosinophils and their degranulation products may enhance pathologic assessment. Future studies are needed to correlate EPX/mm2 with symptoms, endoscopic findings, and esophageal distensibility.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Wright BL,Doyle AD,Shim KP,Pai RK,Barshow SM,Horsley-Silva JL,Luo H,Rank MA,Jacobsen EA,Katzka DA,Kita H,Dellon ESdoi
10.1007/s10620-020-06230-5subject
Has Abstractpub_date
2020-04-04 00:00:00eissn
0163-2116issn
1573-2568pii
10.1007/s10620-020-06230-5pub_type
杂志文章abstract::The optimal administration of immunosuppressants such as tacrolimus (Tac) for small-for-size (SFS) grafts, where the functional liver mass is small and must regenerate, has not been reported so far. The aim of this study is to clarify the characteristics of Tac metabolism according to liver volume. Seven-week-old male...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-008-0551-0
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
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journal_title:Digestive diseases and sciences
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doi:10.1007/s10620-010-1530-9
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journal_title:Digestive diseases and sciences
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journal_title:Digestive diseases and sciences
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doi:10.1007/BF01536411
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journal_title:Digestive diseases and sciences
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journal_title:Digestive diseases and sciences
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journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章
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journal_title:Digestive diseases and sciences
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journal_title:Digestive diseases and sciences
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journal_title:Digestive diseases and sciences
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journal_title:Digestive diseases and sciences
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更新日期:1993-07-01 00:00:00
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doi:10.1007/s10620-005-2700-z
更新日期:2005-06-01 00:00:00
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journal_title:Digestive diseases and sciences
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