Abstract：
BACKGROUND/AIMS:The purpose of this study is to explore the inhibition or activation effects of microRNA-146 B on the expression of PTP1B in gastric cancer cells. METHODS:The expressions of PTP1B and miR-146b in gastric cancer were detected by RT-qPCR. The effects of miR-146b on cell apoptosis and proliferation of gastric cancer were detected. The methods used in the detection process included Annexin V/PI dying method, colony formation assay, and MTT assay. The downstream target gene miR-146b was predicted and screened by bioinformatics and luciferase reporter assay. The mRNA and protein expressions of the target gene PTP1B miR-146b were determined using RT-qPCR and western blot. The expression of miR-146 B in mice was detected by the cells transfected with microRNA-146 B in vivo. RESULTS:Compared with normal tissues, PTP1B was higher and miR-146b was lower in cancer cells. Over-expression of miR-146b can inhibit cell viability and increase the apoptosis rate. According to the luciferase reporter assay, PTP1B was the downstream target gene of miR-146b. The re-introduction of PTP1B reversed the growth inhibition and apoptosis of gastric cancer cells induced by miR-146b. From the mouse xenograft model, the over-expression of miR-146b inhibited the tumor growth and reduced the expression level of PTP1B. CONCLUSION:miR-146b directly inhibits the expression of PTP1B and suppressed the growth and development of gastric cancer.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Xu J,Zhang Z,Chen Q,Yang L,Yin Jdoi
10.1007/s10620-019-05771-8subject
Has Abstractpub_date
2020-02-01 00:00:00pages
457-463issue
2eissn
0163-2116issn
1573-2568pii
10.1007/s10620-019-05771-8journal_volume
65pub_type
杂志文章abstract：:We postulated that coadministration of cholylsarcosine with ursodeoxycholic acid might provide additional benefit to primary biliary cirrhosis patients with an incomplete response to ursodeoxycholic acid. Our aim was to test the tolerability and the effect of adjuvant cholylsarcosine on liver tests and plasma choleste...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1018868126743
更新日期：1998-06-01 00:00:00
abstract：:Stimulation of the intrinsic nerves of the esophageal body leads to a twitch of the circular muscle after the end of the stimulation (the esophageal off-response), and the twitch spreads in the distal direction because of a latency gradient in the onset of the off-response between the proximal and the distal esophagus...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01536324
更新日期：1989-04-01 00:00:00
abstract：BACKGROUND:Antiviral therapy in addition to immunoprophylaxis at birth has been shown to further reduce perinatal transmission of hepatitis B virus (HBV) in highly viremic women. AIMS:The aim of this study was to describe the use of tenofovir disoproxil fumarate (TDF) prophylaxis to reduce maternal HBV DNA levels and ...
journal_title：Digestive diseases and sciences
pub_type： 临床试验,杂志文章
doi：10.1007/s10620-014-3230-3
更新日期：2014-11-01 00:00:00
abstract：:We have investigated the presence, duration, and clinical significance of granulocyte accumulation, using indium-111 granulocyte scanning, in patients following uncomplicated intestinal anastomosis. Eight patients underwent intestinal resection and anastomosis (right hemicolectomy, 5; sigmoid colectomy, 2; ileal resec...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01318118
更新日期：1986-03-01 00:00:00
abstract：:Little information on functional status and well-being is available in patients with functional gastrointestinal disease. We aimed to evaluate whether quality of life is poorer in patients with functional dyspepsia. A consecutive sample of 73 patients with functional dyspepsia completed a validated questionnaire prior...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02064375
更新日期：1995-03-01 00:00:00
abstract：:The diagnostic values in detecting terminal ileum dysfunction using [75Se]HCAT have been assessed for two different scintigraphic techniques in 58 subjects. The measurement of [75Se]HCAT T1/2 in the enterohepatic circulation by daily gallbladder scintigraphy showed 78% sensitivity, 96% specificity, 96% positive predic...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01308176
更新日期：1992-02-01 00:00:00
abstract：:Abnormalities in colonic glycoprotein synthesis have been implicated in the pathogenesis of ulcerative colitis and Crohn's disease. Glucosamine synthetase is the rate-limiting step in the biosynthesis of gastrointestinal glycoprotein and has been measured in control subjects (N = 23) and patients with ulcerative colit...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02088339
更新日期：1994-03-01 00:00:00
abstract：:Since the publication of the last extensive review of hepatitis vaccines, use of inactivated hepatitis A vaccines has been extended to high-risk regions of the United States and specific patient groups, such as those with chronic liver disease, and use of the recombinant hepatitis B vaccines has been recommended for o...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1023/a:1015397807278
更新日期：2002-06-01 00:00:00
abstract：:In order to investigate whether the human exocrine pancreas is capable of adapting to a diet with a high-carbohydrate, low-fat, and normal protein content, 10 healthy subjects were given a continuous intraduodenal infusion of such a dietary composition (8760 kJ in 2400 ml/day) via a portable infusion pump over a perio...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01347884
更新日期：1985-03-01 00:00:00
abstract：BACKGROUND:Progressive hepatic fibrosis with development of cirrhosis is a feature of almost all chronic liver diseases. AIMS:We assessed the performance of Fibroscan in patients with chronic viral hepatitis, and in comparison with and combined with several surrogate serum markers for predicting cirrhosis. METHODS:In...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-010-1219-0
更新日期：2010-12-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/b:ddas.0000007860.87503.09
更新日期：2003-12-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-010-1522-9
更新日期：2011-06-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01318195
更新日期：1991-03-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1007/s10620-005-9011-2
更新日期：2007-11-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1005504311190
更新日期：2000-10-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01317086
更新日期：1984-12-01 00:00:00
abstract：:After gastrectomy, Roux-Y limb reconstruction leads to duodenojejunal motor disturbances. Because motilin and pancreatic polypeptide (PP) play a role in the regulation of digestive motility, their plasma concentrations were determined in rats after Roux-Y gastrectomy. Three months after a distal Roux-Y gastrectomy, co...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1005548524334
更新日期：2000-08-01 00:00:00
abstract：:Patients with chronic epigastric to right upper quadrant pain are often considered to have gallbladder of sphincter of Oddi dysfunction, but standard tests are nondiagnostic. In 62 consecutive patients with this compliant undergoing antroduodenal manometry, we correlated a change on duodenal motility with spasm of the...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02212692
更新日期：1995-08-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1024788428692
更新日期：2003-08-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01299895
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01295907
更新日期：1993-05-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-018-5112-6
更新日期：2018-11-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01347905
更新日期：1986-01-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-016-4270-7
更新日期：2016-12-01 00:00:00
abstract：:NSAID use and Helicobacter pylori both cause damage to the gastric mucosa and can cause peptic ulcers. Our aim was to test the relationship between gastric mucosal polymorphonuclear leukocyte (PMN) infiltration and the severity of NSAID-induced gastric injury. H. pylori density, mucosal interleukin-8 (IL-8), and nitri...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1013251100950
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:Controlled attenuation parameter (CAP) has been developed to estimate the extent of hepatic steatosis. AIMS:The purpose was to evaluate the usefulness of CAP in assessing hepatic steatosis and its change using magnetic resonance imaging proton density fat fraction (MRI-PDFF) as reference standard. METHODS:...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-019-05883-1
更新日期：2020-05-01 00:00:00
abstract：:The authors have developed a primate model for evaluating cytoprotection against alcohol-induced injury in gastric mucosa pretreated with antiulcer agents. In the present study, groups of squirrel monkeys received either a single oral dose of misoprostol (500 or 200 micrograms/kg), cimetidine (3 or 6 mg/kg), placebo, ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01309333
更新日期：1986-02-01 00:00:00
abstract：BACKGROUND:It is not known whether transjugular intrahepatic porto-systemic shunt (TIPS) is safe in patients with advanced liver cirrhosis. The aim of our study was to evaluate the impact of TIPS on transplant-free survival in patients with liver cirrhosis and MELD score ≥15. METHODS:All adult patients who underwent T...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-016-4185-3
更新日期：2017-02-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 临床试验,杂志文章
doi：10.1007/BF01535939
更新日期：1988-12-01 00:00:00
abstract：:The purpose of this study was to evaluate the inhibitory activity of selenium-enriched probiotics against pathogenic Escherichia coli (E. coli) in vitro and in vivo. Escherichia coli was co-cultured in vitro with each probiotic strain individually, and a mixture of the four strains and its population was counted at va...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-008-0361-4
更新日期：2009-02-01 00:00:00
