Abstract:
:Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissiveness, viral replication and interferon (IFN) synthesis was evaluated. BVDV replicated 3-4 logs lower in AT cells with diminished antigen deposition compared to BT cells. BVDV infection inhibited IFN response in both AT and BT cells. Compared to BT cells, BVDV-infected AT cells had a 2-5 fold increase in IFN synthesis following dsRNA stimulation. The greater IFN response of AT cells compared to BT cells following poly I:C stimulation with or without ncp BVDV infection, may be the basis for the decreased BVDV permissiveness of AT cells and may contribute to the clinical differences following BVDV infection of alpacas and cattle.
journal_name
Virologyjournal_title
Virologyauthors
Samson HC,Topliff CL,Donis RO,Kelling CLdoi
10.1016/j.virol.2011.02.006subject
Has Abstractpub_date
2011-04-25 00:00:00pages
111-7issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(11)00067-5journal_volume
413pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::New BVDV strains associated with very high mortalities, which killed about 25% of the veal calves in Quebec in 1993, have been isolated. In this study, characterization of the last two-thirds of the 5' untranslated region (5'UTR) of their genome and virus neutralization experiments with polyvalent antisera raised in d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1483
更新日期:1994-09-01 00:00:00
abstract::Transient expression studies using a Nicotiana benthamiana suspension cell-derived protoplast system have identified a minimal sequence that is necessary and sufficient for activation of the tomato golden mosaic virus coat protein (CP) promoter by the viral TrAP protein (also called AL2). The sequence has a bipartite ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1757
更新日期:2003-01-20 00:00:00
abstract::The hypervariable region 1 (HVR1) of the putative envelope 2 protein of the hepatitis C virus (HCV) is the most variable part of the whole HCV polyprotein. Anti-HVR1 antibodies have been shown to protect against HCV infection, indicating that this region contains an important neutralization determinant. Recently we an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0238
更新日期:2000-04-10 00:00:00
abstract::The DNA polymerase (DNApol) and major capsid protein (MCP) genes were used as models to study promoter activity in Chilo iridescent virus (CIV). Infection of Bombyx mori SPC-BM-36 cells in the presence of inhibitors of DNA or protein synthesis showed that DNApol, as well as helicase, is an immediate-early gene and con...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.08.007
更新日期:2003-12-20 00:00:00
abstract::Natural infection and vaccination with a live-attenuated measles virus (MV) induce CD8(+) T-cell-mediated immune responses that may play a central role in controlling MV infection. In this study, we show that newly identified human HLA-A2 epitopes from MV hemagglutinin (H) and fusion (F) proteins induced protective im...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.040
更新日期:2006-09-01 00:00:00
abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.08.032
更新日期:2016-11-01 00:00:00
abstract::Serotype 4 rotavirus strains have been classified into two antigenic "subtypes" by a solid phase immune electron microscopy technique in which cross-absorbed animal polyclonal immune sera are used as the source of antibodies. The sequences of the gene encoding the outer capsid glycoprotein VP7 from a single serotype 4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90766-i
更新日期:1992-05-01 00:00:00
abstract::Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90130-2
更新日期:1984-05-01 00:00:00
abstract::Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1389
更新日期:2002-03-30 00:00:00
abstract::The Ns strain of Cucumber mosaic virus (CMV) induces hypersensitive response (HR) on Nicotiana tabacum cv. Xanthi-nc and on Nicotiana glutinosa. The genetic determinant of the HR induction was localized earlier to amino acid 461 of the 1a protein. The 3D structure of the 1a protein is still unknown and building a homo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.014
更新日期:2007-02-05 00:00:00
abstract::Primate lentiviruses are composed of several distinct lineages, including human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus SIVagm. HIV-1 and HIV-2 have significant differences in the mechanisms of viral RNA encapsidation. Therefore, the RNA packaging mechanisms of SIVagm cannot be ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.030
更新日期:2007-06-20 00:00:00
abstract::The DNAs from the granulosis viruses of Plodia interpunctella and Pieris rapae were clearly distinguishable based on restriction endonuclease fragment patterns. By sizing single- and double-digestion products approximate molecular weights of 72 x 10(2) and 75 x 10(6), respectively, were estimated for the viral genomes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90357-8
更新日期:1980-07-30 00:00:00
abstract::To celebrate the sixtieth anniversary of Virology a survey is made of the plant viruses, virologists and their institutions, and tools and technology described in the first decade of plant virus publications in Virology. This was a period when plant viruses increasingly became tools of discovery as epistemic objects a...
journal_title:Virology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.virol.2015.03.019
更新日期:2015-05-01 00:00:00
abstract::One of the newest niches for baculoviruses-based technologies is their use as vectors for mammalian cell transduction and gene therapy applications. However, an outstanding safety issue related to such use is the residual expression of viral genes in infected mammalian cells. Here we show that infectious baculoviruses...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.07.020
更新日期:2010-10-25 00:00:00
abstract::In wild-type human parainfluenza virus type 2 (WT HPIV2), one gene (the P/V gene) encodes both the polymerase-associated phosphoprotein (P) and the accessory V protein. We generated a HPIV2 virus (rHPIV2-V(ko)) in which the P/V gene encodes only the P protein to examine the role of V in replication in vivo and as a po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.018
更新日期:2010-02-20 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) UL37 open reading frame encodes a 120-kDa late (gamma 1) phosphoprotein in infected cells. Analysis of isolated mature HSV virions and light particles revealed that the UL37 protein is a component of the virion. Detergent solubilization and protease digestion experiments suggest...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1028
更新日期:1995-02-01 00:00:00
abstract::Bacteriophage P2 integrase (Int) mediates site-specific recombination leading to integration or excision of the phage genome in or out of the bacterial chromosome. Int belongs to the large family of tyrosine recombinases that have two different DNA recognition motifs binding to the arm and core sites, respectively, wh...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.11.015
更新日期:2005-02-05 00:00:00
abstract::The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8903
更新日期:1998-01-05 00:00:00
abstract::The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of int...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90494-c
更新日期:1990-07-01 00:00:00
abstract::HIV CRF07 B'/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.07.024
更新日期:2013-11-01 00:00:00
abstract::The HIV-1 Tat protein is released by infected cells and has numerous biological activities which might contribute either to the impairment of the immune response or to viral dissemination and pathogenesis. To date, the effects of Tat protein on metabolites remain unclear. In this study, a metabolomic study on serum of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.11.005
更新日期:2012-01-20 00:00:00
abstract::Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1069
更新日期:1994-02-01 00:00:00
abstract::Phylogenetic analysis of the N8 neuraminidase (NA) genes from 18 influenza A viruses, representing equine and avian hosts in different geographic locations, revealed three major lineages: (i) currently circulating equine 2 viruses; (ii) avian viruses isolated in the Eurasian region, including A/Equine/Jilin/1/89, a re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1196
更新日期:1993-04-01 00:00:00
abstract::The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90155-9
更新日期:1988-09-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.07.009
更新日期:2003-12-20 00:00:00
abstract::The genome of peanut clump pecluvirus (PCV) consists of two messenger RNA components which contain, respectively, three and five open reading frames (ORFs). Inoculation of transcripts from full-length cDNA clones derived from the PCV RNAs showed that RNA-1 is able to replicate in the absence of RNA-2 in protoplasts, b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9287
更新日期:1998-09-01 00:00:00
abstract::Human cytomegalovirus (HCMV) glycoprotein B (gB) promotes virion entry into cells by fusing the virion envelope with the cellular membrane. We recently reported that UB cells (U373 glioblastoma cells constructed to produce HCMV gB constitutively) form multinucleate syncytia that are dependent on the density of gB in t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1290
更新日期:1995-06-01 00:00:00
abstract::We have characterized capsids made by seven temperature-sensitive (ts) mutants of HSV-1 previously shown to be defective in viral DNA processing and packaging at the nonpermissive temperature (NPT). The empty capsids isolated from mutant-infected cells at the NPT were devoid of DNA, cosedimented in sucrose with wt B c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90288-7
更新日期:1988-04-01 00:00:00
abstract::Ribavirin is the first synthetic, broad-spectrum antiviral nucleoside. Despite its more than 30 year history, the mechanism of action of this compound remains unclear and somewhat controversial. Recent data suggest the possibility that the activity of ribavirin against RNA viruses is a reflection of incorporation of r...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1006/viro.2002.1487
更新日期:2002-07-05 00:00:00