Abstract:
:One of the newest niches for baculoviruses-based technologies is their use as vectors for mammalian cell transduction and gene therapy applications. However, an outstanding safety issue related to such use is the residual expression of viral genes in infected mammalian cells. Here we show that infectious baculoviruses lacking the major transcriptional regulator, IE1, can be produced in insect host cells stably transformed with IE1 expression constructs lacking targets of homologous recombination that could promote the generation of wt-like revertants. Such ie1-deficient baculoviruses are unable to direct viral gene transcription to any appreciable degree and do not replicate in normal insect host cells. Most importantly, the residual viral gene expression, which occurs in mammalian cells infected with wt baculoviruses is reduced 10 to 100 fold in cells infected with ie1-deficient baculoviruses. Thus, ie1-deficient baculoviruses offer enhanced safety features to baculovirus-based vector systems destined for use in gene therapy applications.
journal_name
Virologyjournal_title
Virologyauthors
Efrose R,Swevers L,Iatrou Kdoi
10.1016/j.virol.2010.07.020subject
Has Abstractpub_date
2010-10-25 00:00:00pages
293-301issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(10)00473-3journal_volume
406pub_type
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