Abstract:
:To better understand the physiology and acclimation capability of the cell, one of the great challenges of the future is to access the interior of a cell and unveil its chemical landscape (composition and distribution of elements and molecules). Chemical imaging has greatly improved in sensitivity and spatial resolution to visualize and quantify nutrients, metabolites, toxic elements, and drugs in single cells at the subcellular level. This review aims to present the current potential of these emerging imaging technologies and to guide biologists towards a strategy for interrogating biological processes at the nanoscale. We also describe various solutions to combine multiple imaging techniques in a correlative way and provide perspectives and future directions for integrative subcellular imaging across different disciplines.
journal_name
Trends Cell Bioljournal_title
Trends in cell biologyauthors
Decelle J,Veronesi G,Gallet B,Stryhanyuk H,Benettoni P,Schmidt M,Tucoulou R,Passarelli M,Bohic S,Clode P,Musat Ndoi
10.1016/j.tcb.2019.12.007subject
Has Abstractpub_date
2020-03-01 00:00:00pages
173-188issue
3eissn
0962-8924issn
1879-3088pii
S0962-8924(19)30221-1journal_volume
30pub_type
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