Abstract：
:Long noncoding RNAs (lncRNAs) have been described in cell lines and various whole tissues, but lncRNA analysis of development in vivo is limited. Here, we comprehensively analyze lncRNA expression for the adult mouse subventricular zone neural stem cell lineage. We utilize complementary genome-wide techniques including RNA-seq, RNA CaptureSeq, and ChIP-seq to associate specific lncRNAs with neural cell types, developmental processes, and human disease states. By integrating data from chromatin state maps, custom microarrays, and FACS purification of the subventricular zone lineage, we stringently identify lncRNAs with potential roles in adult neurogenesis. shRNA-mediated knockdown of two such lncRNAs, Six3os and Dlx1as, indicate roles for lncRNAs in the glial-neuronal lineage specification of multipotent adult stem cells. Our data and workflow thus provide a uniquely coherent in vivo lncRNA analysis and form the foundation of a user-friendly online resource for the study of lncRNAs in development and disease.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Ramos AD,Diaz A,Nellore A,Delgado RN,Park KY,Gonzales-Roybal G,Oldham MC,Song JS,Lim DAdoi
10.1016/j.stem.2013.03.003subject
Has Abstractpub_date
2013-05-02 00:00:00pages
616-28issue
5eissn
1934-5909issn
1875-9777pii
S1934-5909(13)00098-2journal_volume
12pub_type
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