Abstract:
:Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of over 2,000 FGCs and their gonadal niche cells in female and male human embryos spanning several developmental stages. We found that female FGCs undergo four distinct sequential phases characterized by mitosis, retinoic acid signaling, meiotic prophase, and oogenesis. Male FGCs develop through stages of migration, mitosis, and cell-cycle arrest. Individual embryos of both sexes simultaneously contain several subpopulations, highlighting the asynchronous and heterogeneous nature of FGC development. Moreover, we observed reciprocal signaling interactions between FGCs and their gonadal niche cells, including activation of the bone morphogenic protein (BMP) and Notch signaling pathways. Our work provides key insights into the crucial features of human FGCs during their highly ordered mitotic, meiotic, and gametogenetic processes in vivo.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Li L,Dong J,Yan L,Yong J,Liu X,Hu Y,Fan X,Wu X,Guo H,Wang X,Zhu X,Li R,Yan J,Wei Y,Zhao Y,Wang W,Ren Y,Yuan P,Yan Z,Hu B,Guo F,Wen L,Tang F,Qiao Jdoi
10.1016/j.stem.2017.03.007subject
Has Abstractpub_date
2017-06-01 00:00:00pages
858-873.e4issue
6eissn
1934-5909issn
1875-9777pii
S1934-5909(17)30078-4journal_volume
20pub_type
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