Abstract：
:With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during preimplantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA replication-dependent dilution both contribute to global paternal DNA demethylation, but demethylation of the maternal genome occurs via replication. Here we present genome-scale DNA methylation maps for both the paternal and maternal genomes of Tet3-depleted and/or DNA replication-inhibited zygotes. In both genomes, we found that inhibition of DNA replication blocks DNA demethylation independently from Tet3 function and that Tet3 facilitates DNA demethylation largely by coupling with DNA replication. For both genomes, our data indicate that replication-dependent dilution is the major contributor to demethylation, but Tet3 plays an important role, particularly at certain loci. Our study thus defines the respective functions of Tet3 and DNA replication in paternal DNA demethylation and reveals an unexpected contribution of Tet3 to demethylation of the maternal genome.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Shen L,Inoue A,He J,Liu Y,Lu F,Zhang Ydoi
10.1016/j.stem.2014.09.002subject
Has Abstractpub_date
2014-10-02 00:00:00pages
459-471issue
4eissn
1934-5909issn
1875-9777pii
S1934-5909(14)00393-2journal_volume
15pub_type
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