The emergence of hematopoietic stem cells is initiated in the placental vasculature in the absence of circulation.

Abstract:

:The mouse placenta was unveiled as an important reservoir for hematopoietic stem cells (HSCs), yet the origin of placental HSCs was unknown. By tracking developing HSCs by expression of Runx1-lacZ and CD41, we have found that HSCs emerge in large vessels in the placenta. Analysis of Ncx1(-/-) embryos, which lack a heartbeat, verified that HSC development is initiated in the placental vasculature independent of blood flow. However, fewer CD41+ hematopoietic cells were found in Ncx1(-/-) placentas than in controls, implying that some HSCs/progenitors colonize the placenta via circulation and/or HSC emergence is compromised without blood flow. Importantly, placentas from Ncx1(-/-) embryos possessed equal potential to generate myelo-erythroid and B and T lymphoid cells upon explant culture, verifying intact multilineage hematopoietic potential, characteristic of developing HSCs. These data suggest that, in addition to providing a niche for a large pool of HSCs prior to liver colonization, the placenta is a true site of HSC generation.

journal_name

Cell Stem Cell

journal_title

Cell stem cell

authors

Rhodes KE,Gekas C,Wang Y,Lux CT,Francis CS,Chan DN,Conway S,Orkin SH,Yoder MC,Mikkola HK

doi

10.1016/j.stem.2008.01.001

subject

Has Abstract

pub_date

2008-03-06 00:00:00

pages

252-63

issue

3

eissn

1934-5909

issn

1875-9777

pii

S1934-5909(08)00002-7

journal_volume

2

pub_type

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