Abstract:
:The mouse placenta was unveiled as an important reservoir for hematopoietic stem cells (HSCs), yet the origin of placental HSCs was unknown. By tracking developing HSCs by expression of Runx1-lacZ and CD41, we have found that HSCs emerge in large vessels in the placenta. Analysis of Ncx1(-/-) embryos, which lack a heartbeat, verified that HSC development is initiated in the placental vasculature independent of blood flow. However, fewer CD41+ hematopoietic cells were found in Ncx1(-/-) placentas than in controls, implying that some HSCs/progenitors colonize the placenta via circulation and/or HSC emergence is compromised without blood flow. Importantly, placentas from Ncx1(-/-) embryos possessed equal potential to generate myelo-erythroid and B and T lymphoid cells upon explant culture, verifying intact multilineage hematopoietic potential, characteristic of developing HSCs. These data suggest that, in addition to providing a niche for a large pool of HSCs prior to liver colonization, the placenta is a true site of HSC generation.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Rhodes KE,Gekas C,Wang Y,Lux CT,Francis CS,Chan DN,Conway S,Orkin SH,Yoder MC,Mikkola HKdoi
10.1016/j.stem.2008.01.001subject
Has Abstractpub_date
2008-03-06 00:00:00pages
252-63issue
3eissn
1934-5909issn
1875-9777pii
S1934-5909(08)00002-7journal_volume
2pub_type
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