High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping.

abstract：:Stem cell niche interactions have been studied extensively with regard to cell polarity and extracellular signaling. Less is known about the way in which signals and polarity cues integrate with intracellular structures to ensure appropriate niche organization and function. Here, we report that nuclear lamins function...

journal_title：Cell stem cell

authors： Chen H,Chen X,Zheng Y

更新日期：2013-07-03 00:00:00

abstract：:Stem cell-based therapies for Parkinson's disease are moving into a new and exciting era, with several groups pursuing clinical trials with pluripotent stem cell (PSC)-derived dopamine neurons. As many groups have ongoing or completed GMP-level cell manufacturing, we highlight key clinical translation considerations f...

journal_title：Cell stem cell

authors： Barker RA,Parmar M,Studer L,Takahashi J

更新日期：2017-11-02 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT) enables cloning of differentiated cells by reprogramming their nuclei to a totipotent state. However, successful full-term development of SCNT embryos is a low-efficiency process and arrested embryos frequently exhibit epigenetic abnormalities. Here, we generated genome-wide DNA me...

journal_title：Cell stem cell

authors： Gao R,Wang C,Gao Y,Xiu W,Chen J,Kou X,Zhao Y,Liao Y,Bai D,Qiao Z,Yang L,Wang M,Zang R,Liu X,Jia Y,Li Y,Zhang Y,Yin J,Wang H,Wan X,Liu W,Zhang Y,Gao S

更新日期：2018-09-06 00:00:00

abstract：:In a pioneering study in New England Journal of Medicine, Schweitzer et al. (2020) report on a patient with Parkinson's disease who received a graft of dopamine neurons obtained from in vitro differentiated induced pluripotent stem cells, derived from the patient's own skin fibroblasts, showing the feasibility of auto...

journal_title：Cell stem cell

authors： Parmar M,Björklund A

更新日期：2020-07-02 00:00:00

abstract：:Individuals with neurofibromatosis type 1 (NF1) develop abnormalities of both neuronal and glial cell lineages, suggesting that the NF1 protein neurofibromin is an essential regulator of neuroglial progenitor function. In this regard, Nf1-deficient embryonic telencephalic neurospheres exhibit increased self-renewal an...

journal_title：Cell stem cell

authors： Hegedus B,Dasgupta B,Shin JE,Emnett RJ,Hart-Mahon EK,Elghazi L,Bernal-Mizrachi E,Gutmann DH

更新日期：2007-10-11 00:00:00

abstract：:Expansion of transposable elements (TEs) coincides with evolutionary shifts in gene expression. TEs frequently harbor binding sites for transcriptional regulators, thus enabling coordinated genome-wide activation of species- and context-specific gene expression programs, but such regulation must be balanced against th...

journal_title：Cell stem cell

authors： Pontis J,Planet E,Offner S,Turelli P,Duc J,Coudray A,Theunissen TW,Jaenisch R,Trono D

更新日期：2019-05-02 00:00:00

abstract：:Clonal tracking of hematopoietic stem and progenitor cells (HSPCs) has proven valuable for studying their behavior in murine recipients. Now in Cell Stem Cell, Kim et al. (2014) and Wu et al. (2014) extend these analyses to nonhuman primates, providing insights into dynamics of HSPC expansion and lineage commitment fo...

journal_title：Cell stem cell

authors： Dykstra B,Bystrykh LV

更新日期：2014-04-03 00:00:00

abstract：:We are only beginning to understand the higher-order chromatin structure of pluripotent stem cells and its relevance to cell fate. Three recent studies used different approaches to reconstruct the 3D chromatin landscape of naive and primed pluripotent cells, unveiling common features as well as differences between the...

journal_title：Cell stem cell

authors： Wei Z,Lu W

更新日期：2016-02-04 00:00:00

abstract：:Recently in Cell, Mitalipov and colleagues report an advance that has eluded scientists for over a decade-the successful derivation of embryonic stem cell lines using somatic cell nuclear transfer, or SCNT (Tachibana et al., 2013). ...

journal_title：Cell stem cell

authors： Trounson A,DeWitt ND

更新日期：2013-06-06 00:00:00

abstract：:Human disease phenotypes associated with haploinsufficient gene requirements are often not recapitulated well in animal models. Here, we have investigated the association between human GATA6 haploinsufficiency and a wide range of clinical phenotypes that include neonatal and adult-onset diabetes using CRISPR (clustere...

journal_title：Cell stem cell

authors： Shi ZD,Lee K,Yang D,Amin S,Verma N,Li QV,Zhu Z,Soh CL,Kumar R,Evans T,Chen S,Huangfu D

更新日期：2017-05-04 00:00:00

abstract：:Recently in Nature Neuroscience, Gaillard et al. (2007) study axonal projections from embryonic cortical explants grafted into acutely damaged adult motor cortex. After attempting to rule out fusion of donor tissue with pre-existing host circuitry, the authors report robust long-distance donor axonal projections and s...

journal_title：Cell stem cell

authors： Singec I,Snyder EY

更新日期：2007-10-11 00:00:00

abstract：:Fetal gene therapy using safe and effective viral vectors no longer remains a distant prospect. Recently in Nature Medicine, Massaro et al. (2018) demonstrated that prenatal intracranial injection of a viral vector results in improved neurologic function, raising the intriguing possibility that in utero gene therapy m...

journal_title：Cell stem cell

authors： MacKenzie TC

更新日期：2018-09-06 00:00:00

abstract：:The study of MSC trafficking is clinically relevant for minimally invasive cell therapy to promote regeneration of damaged tissue, to treat inflammation, and to promote angiogenesis. However, these studies are complicated by the diverse methods used to culture, characterize, and deliver MSCs and by the variety of meth...

journal_title：Cell stem cell

authors： Karp JM,Leng Teo GS

更新日期：2009-03-06 00:00:00

abstract：:Radiation is one of the most effective cancer treatments. However, gastrointestinal (GI) syndrome is a major limiting factor in abdominal and pelvic radiotherapy. The loss of crypt stem cells or villus endothelial cells has been suggested to be responsible for radiation-induced intestinal damage. We report here a crit...

journal_title：Cell stem cell

authors： Qiu W,Carson-Walter EB,Liu H,Epperly M,Greenberger JS,Zambetti GP,Zhang L,Yu J

更新日期：2008-06-05 00:00:00

abstract：:Leukemia stem cells (LSCs) are resistant to current therapies used to treat chronic myeloid leukemia (CML). Abraham et al. (2016) have identified a molecular network critical for CML LSC survival and propose that simultaneously targeting two of their major transcriptional regulators, p53 and c-Myc, may facilitate thei...

journal_title：Cell stem cell

authors： Carvajal LA,Steidl U

更新日期：2016-07-07 00:00:00

abstract：:Mouse embryonic stem cells (mESCs) cultured under serum/LIF conditions exhibit heterogeneous expression of pluripotency-associated factors that can be overcome by two inhibitors (2i) of the MEK and GSK3 pathways. Several studies have shown that the "ground state" induced by 2i is characterized by global hypomethylatio...

journal_title：Cell stem cell

authors： Galonska C,Ziller MJ,Karnik R,Meissner A

更新日期：2015-10-01 00:00:00

abstract：:The cellular identity of niche cells that regulate hematopoietic stem cell (HSC) self-renewal and differentiation has been debated for several years. Two recent studies in Nature (Ding and Morrison, 2013; Greenbaum et al., 2013) have shed light into the bone marrow stromal subsets making CXCL12, a chemokine critical f...

journal_title：Cell stem cell

authors： Hanoun M,Frenette PS

更新日期：2013-04-04 00:00:00

abstract：:To date, pluripotent epiblast stem cells (EpiSCs) had only been derived from postimplantation mouse embryos. In this issue of Cell Stem Cell, Najm et al. (2011) demonstrate that EpiSCs can be routinely derived from preimplantation embryos, showing that both human and mouse blastocysts can produce the same class of pri...

journal_title：Cell stem cell

authors： Kunath T

更新日期：2011-03-04 00:00:00

abstract：:The mechanisms that regulate the establishment of adult stem cell pools during normal and perturbed mammalian development are still largely unknown. Here, we asked whether a maternal cytokine surge, which occurs during human maternal infections and has been implicated in cognitive disorders, might have long-lasting co...

journal_title：Cell stem cell

authors： Gallagher D,Norman AA,Woodard CL,Yang G,Gauthier-Fisher A,Fujitani M,Vessey JP,Cancino GI,Sachewsky N,Woltjen K,Fatt MP,Morshead CM,Kaplan DR,Miller FD

更新日期：2013-11-07 00:00:00

abstract：:While studies of organ development have traditionally relied on model organisms, recent advances in embryonic stem cell (ESC) culture allow investigation of organogenesis in human cells. Wang et al. (2015) employ this system to map the dynamic enhancer landscape during ESC differentiation to the endoderm derivatives p...

journal_title：Cell stem cell

authors： Kaestner KH

更新日期：2015-04-02 00:00:00

abstract：:Reconstructing lineage relationships between cells within a tissue or organism is a long-standing aim in biology. Traditionally, lineage tracing has been achieved through the (genetic) labeling of a cell followed by the tracking of its offspring. Currently, lineage trajectories can also be predicted using single-cell ...

journal_title：Cell stem cell

authors： Kester L,van Oudenaarden A

更新日期：2018-08-02 00:00:00

abstract：:The identification of tumor-initiating cells represents a significant challenge for studies of prostate cancer. In a recent issue of Cell Reports, Agarwal and colleagues use organoid culture to ascertain two distinct luminal progenitors in a mouse model of prostate cancer, shedding new light on lineage relationships i...

journal_title：Cell stem cell

authors： Shen MM

更新日期：2015-12-03 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) is a developmental process important for cell fate determination. Fibroblasts, a product of EMT, can be reset into induced pluripotent stem cells (iPSCs) via exogenous transcription factors but the underlying mechanism is unclear. Here we show that the generation of iPSCs fro...

journal_title：Cell stem cell

authors： Li R,Liang J,Ni S,Zhou T,Qing X,Li H,He W,Chen J,Li F,Zhuang Q,Qin B,Xu J,Li W,Yang J,Gan Y,Qin D,Feng S,Song H,Yang D,Zhang B,Zeng L,Lai L,Esteban MA,Pei D

更新日期：2010-07-02 00:00:00

abstract：:Although the clinical outcomes of cell therapy trials have not met initial expectations, emerging evidence suggests that injury-mediated tissue damage might benefit from the delivery of cells or their secreted products. Pluripotent stem cells (PSCs) are promising cell sources primarily because of their capacity to gen...

journal_title：Cell stem cell

authors： Desgres M,Menasché P

更新日期：2019-11-07 00:00:00

abstract：:Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) can be achieved by viral-mediated transduction of defined transcription factors. Moving toward the eventual goal of clinical application, it is necessary to overcome limitations such as low reprogramming efficiency and genomic alterations due to ...

journal_title：Cell stem cell

authors： Feng B,Ng JH,Heng JC,Ng HH

更新日期：2009-04-03 00:00:00

abstract：:Cellular reprogramming converts differentiated cells into induced pluripotent stem cells (iPSCs). However, this process is typically very inefficient, complicating mechanistic studies. We identified and molecularly characterized rare, early intermediates poised to reprogram with up to 95% efficiency, without perturbin...

journal_title：Cell stem cell

authors： Schwarz BA,Cetinbas M,Clement K,Walsh RM,Cheloufi S,Gu H,Langkabel J,Kamiya A,Schorle H,Meissner A,Sadreyev RI,Hochedlinger K

更新日期：2018-08-02 00:00:00

abstract：:Although distinct human induced pluripotent stem cell (hiPSC) lines can display considerable epigenetic variation, it has been unclear whether such variability impacts their utility for disease modeling. Here, we show that although low-passage female hiPSCs retain the inactive X chromosome of the somatic cell they are...

journal_title：Cell stem cell

authors： Mekhoubad S,Bock C,de Boer AS,Kiskinis E,Meissner A,Eggan K

更新日期：2012-05-04 00:00:00

abstract：:Human induced pluripotent stem cells (hiPSCs) hold promise for myocardial repair following injury, but preclinical studies in large animal models are required to determine optimal cell preparation and delivery strategies to maximize functional benefits and to evaluate safety. Here, we utilized a porcine model of acute...

journal_title：Cell stem cell

authors： Ye L,Chang YH,Xiong Q,Zhang P,Zhang L,Somasundaram P,Lepley M,Swingen C,Su L,Wendel JS,Guo J,Jang A,Rosenbush D,Greder L,Dutton JR,Zhang J,Kamp TJ,Kaufman DS,Ge Y,Zhang J

更新日期：2014-12-04 00:00:00

abstract：:Hematopoietic stem cells (HSCs) must at times exit quiescence to divide and produce differentiated blood cells. In this issue of Cell Stem Cell, Takubo et al. (2013) and Yu et al. (2013) show that these opposing actions require distinct metabolic programs to meet the changing energy demands of self-renewing HSCs. ...

journal_title：Cell stem cell

authors： Warr MR,Passegué E

更新日期：2013-01-03 00:00:00

abstract：:Mammalian skin is a complex and heterogeneous tissue with several distinct compartments and stem cell populations. Joost et al. (2016) now use single-cell RNaseq to comprehensively reconstruct this complexity, revealing spatial and pseudotemporal differences between transcriptional programs in distinct compartments an...

journal_title：Cell stem cell

authors： Chen JC,Christiano AM

更新日期：2016-10-06 00:00:00