Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells.

Abstract:

:Human induced pluripotent stem cells (hiPSCs) hold promise for myocardial repair following injury, but preclinical studies in large animal models are required to determine optimal cell preparation and delivery strategies to maximize functional benefits and to evaluate safety. Here, we utilized a porcine model of acute myocardial infarction (MI) to investigate the functional impact of intramyocardial transplantation of hiPSC-derived cardiomyocytes, endothelial cells, and smooth muscle cells, in combination with a 3D fibrin patch loaded with insulin growth factor (IGF)-encapsulated microspheres. hiPSC-derived cardiomyocytes integrated into host myocardium and generated organized sarcomeric structures, and endothelial and smooth muscle cells contributed to host vasculature. Trilineage cell transplantation significantly improved left ventricular function, myocardial metabolism, and arteriole density, while reducing infarct size, ventricular wall stress, and apoptosis without inducing ventricular arrhythmias. These findings in a large animal MI model highlight the potential of utilizing hiPSC-derived cells for cardiac repair.

journal_name

Cell Stem Cell

journal_title

Cell stem cell

authors

Ye L,Chang YH,Xiong Q,Zhang P,Zhang L,Somasundaram P,Lepley M,Swingen C,Su L,Wendel JS,Guo J,Jang A,Rosenbush D,Greder L,Dutton JR,Zhang J,Kamp TJ,Kaufman DS,Ge Y,Zhang J

doi

10.1016/j.stem.2014.11.009

subject

Has Abstract

pub_date

2014-12-04 00:00:00

pages

750-61

issue

6

eissn

1934-5909

issn

1875-9777

pii

S1934-5909(14)00521-9

journal_volume

15

pub_type

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