Abstract：
:Human induced pluripotent stem cells (hiPSCs) hold promise for myocardial repair following injury, but preclinical studies in large animal models are required to determine optimal cell preparation and delivery strategies to maximize functional benefits and to evaluate safety. Here, we utilized a porcine model of acute myocardial infarction (MI) to investigate the functional impact of intramyocardial transplantation of hiPSC-derived cardiomyocytes, endothelial cells, and smooth muscle cells, in combination with a 3D fibrin patch loaded with insulin growth factor (IGF)-encapsulated microspheres. hiPSC-derived cardiomyocytes integrated into host myocardium and generated organized sarcomeric structures, and endothelial and smooth muscle cells contributed to host vasculature. Trilineage cell transplantation significantly improved left ventricular function, myocardial metabolism, and arteriole density, while reducing infarct size, ventricular wall stress, and apoptosis without inducing ventricular arrhythmias. These findings in a large animal MI model highlight the potential of utilizing hiPSC-derived cells for cardiac repair.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Ye L,Chang YH,Xiong Q,Zhang P,Zhang L,Somasundaram P,Lepley M,Swingen C,Su L,Wendel JS,Guo J,Jang A,Rosenbush D,Greder L,Dutton JR,Zhang J,Kamp TJ,Kaufman DS,Ge Y,Zhang Jdoi
10.1016/j.stem.2014.11.009subject
Has Abstractpub_date
2014-12-04 00:00:00pages
750-61issue
6eissn
1934-5909issn
1875-9777pii
S1934-5909(14)00521-9journal_volume
15pub_type
杂志文章相关文献
Cell Stem Cell文献大全abstract：:In many tissues, homeostasis is maintained by physical contact between stem cells and an anatomically defined niche. However, how stem cell homeostasis is achieved in environments where cells are motile and dispersed among their progeny remains unknown. Using murine spermatogenesis as a model, we find that spermatogen...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2018.11.013
更新日期：2019-01-03 00:00:00
abstract：:Mammalian skin is a complex and heterogeneous tissue with several distinct compartments and stem cell populations. Joost et al. (2016) now use single-cell RNaseq to comprehensively reconstruct this complexity, revealing spatial and pseudotemporal differences between transcriptional programs in distinct compartments an...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2016.09.009
更新日期：2016-10-06 00:00:00
abstract：:Stress can activate tumor-suppressive mechanisms, causing the loss of adult stem cell function with age. In this issue of Cell Stem Cell and in Nature, Castilho et al. (2009) and Harrison et al. (2009) highlight the importance of mTOR signaling in stem cell exhaustion and mammalian aging, respectively. ...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2009.08.008
更新日期：2009-09-04 00:00:00
abstract：:Acute Myelogenous Leukemia (AML) is an aggressive cancer that strikes both adults and children and is frequently resistant to therapy. Thus, identifying signals needed for AML propagation is a critical step toward developing new approaches for treating this disease. Here, we show that Tetraspanin 3 is a target of the ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2015.06.006
更新日期：2015-08-06 00:00:00
abstract：:Like politics, most developmental signals are local. However, in this issue of Cell Stem Cell, Kwan et al. (2016) and colleagues describe how a stress-induced signal that originates in the zebrafish brain promotes the formation of blood at a distant site, the dorsal aorta. ...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2016.08.011
更新日期：2016-09-01 00:00:00
abstract：:Cellular reprogramming converts differentiated cells into induced pluripotent stem cells (iPSCs). However, this process is typically very inefficient, complicating mechanistic studies. We identified and molecularly characterized rare, early intermediates poised to reprogram with up to 95% efficiency, without perturbin...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2018.06.013
更新日期：2018-08-02 00:00:00
abstract：:Similarities between basal cell carcinoma (BCC) tumor cells and hair follicle keratinocytes had previously suggested that BCC originates within the hair follicle bulge stem cell niche. However, in the current Nature Cell Biology, Youssef et al. (2010) show that BCC instead originates in the interfollicular epidermis. ...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2010.03.009
更新日期：2010-04-02 00:00:00
abstract：:Emerging evidence strongly suggests that stem cells and their differentiated progeny display distinct metabolic profiles, but how metabolic changes are coupled with organogenesis has remained unclear. Homem et al. (2014) now reveal a hormone-dependent pathway that couples metabolic changes with stem cell differentiati...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2014.08.006
更新日期：2014-09-04 00:00:00
abstract：:Inducing immune tolerance to prevent rejection is a key step toward successful engraftment of stem-cell-derived tissue in a clinical setting. Using human pluripotent stem cells to generate thymic epithelial cells (TECs) capable of supporting T cell development represents a promising approach to reach this goal; howeve...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2013.04.004
更新日期：2013-08-01 00:00:00
abstract：:In this issue of Cell Stem Cell, Csaszar et al. (2012) develop a culture method that overcomes current limitations in ex vivo hematopoietic stem/progenitor cell expansion by continuously diluting inhibitory signaling factors and maintaining stem cell density. This approach enhances the generation of precursors with po...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2012.01.012
更新日期：2012-02-03 00:00:00
abstract：:Recently in Cell, Mitalipov and colleagues report an advance that has eluded scientists for over a decade-the successful derivation of embryonic stem cell lines using somatic cell nuclear transfer, or SCNT (Tachibana et al., 2013). ...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2013.05.022
更新日期：2013-06-06 00:00:00
abstract：:Mouse embryonic stem cells (mESCs) cultured under serum/LIF conditions exhibit heterogeneous expression of pluripotency-associated factors that can be overcome by two inhibitors (2i) of the MEK and GSK3 pathways. Several studies have shown that the "ground state" induced by 2i is characterized by global hypomethylatio...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2015.07.005
更新日期：2015-10-01 00:00:00
abstract：:Ablation of LGR5+ intestinal stem cells (ISCs) is associated with rapid restoration of the ISC compartment. Different intestinal crypt populations dedifferentiate to provide new ISCs, but the transcriptional and signaling trajectories that guide this process are unclear, and a large body of work suggests that quiescen...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2019.12.011
更新日期：2020-03-05 00:00:00
abstract：:Understanding the fetal hepatic niche is essential for optimizing the generation of functional hepatocyte-like cells (hepatic cells) from human embryonic stem cells (hESCs). Here, we show that KDR (VEGFR2/FLK-1), previously assumed to be mostly restricted to mesodermal lineages, marks a hESC-derived hepatic progenitor...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2013.04.026
更新日期：2013-06-06 00:00:00
abstract：:The emerging field of regenerative rehabilitation integrates biological and bioengineering advances in regenerative medicine with rehabilitative sciences. Here we highlight recent stem cell-based examples of the regenerative rehabilitation paradigm to promote tissue repair and regeneration, and we discuss remaining ch...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2018.02.003
更新日期：2018-03-01 00:00:00
abstract：:Two groups recently reported the in vitro differentiation of human embryonic stem cells into insulin-secreting cells, achieving an elusive goal for regenerative medicine. Herein we provide a perspective regarding these developments, compare phenotypes of the insulin-containing cells to human β cells, and discuss impli...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2014.10.012
更新日期：2014-11-06 00:00:00
abstract：:Bmi1 is required for efficient self-renewal of hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs). In this study, we investigated whether leukemia-associated fusion proteins, which differ in their ability to activate Hox expression, could initiate leukemia in the absence of Bmi1. AML1-ETO and PLZF-RARα, wh...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2011.05.004
更新日期：2011-06-03 00:00:00
abstract：:Most disease variants lie within noncoding genomic regions, making their functional interpretation challenging. Because chromatin openness strongly influences transcriptional activity, we hypothesized that cell-type-specific open chromatin regions (OCRs) might highlight disease-relevant noncoding sequences. To investi...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2017.07.008
更新日期：2017-09-07 00:00:00
abstract：:The identification of tumor-initiating cells represents a significant challenge for studies of prostate cancer. In a recent issue of Cell Reports, Agarwal and colleagues use organoid culture to ascertain two distinct luminal progenitors in a mouse model of prostate cancer, shedding new light on lineage relationships i...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2015.11.018
更新日期：2015-12-03 00:00:00
abstract：:3D organoids enable in vitro human brain development models, but they have not yet recapitulated some essential features of brain circuit formation. Recently, several studies appearing in Nature, Nature Methods, and Cell Stem Cell generated fused organoid models of inhibitory and excitatory neuron development, which c...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2017.08.017
更新日期：2017-09-07 00:00:00
abstract：:Two recent papers in Cell Stem Cell by Soliman et al. (2020) and Scott et al. (2019) explore the contributions of mesenchymal progenitor cells to fibrosis in heart and skeletal muscle. They find tissue-dependent roles for a subpopulation of mesenchymal progenitors expressing Hic1 with varied differentiation capacities...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2019.12.010
更新日期：2020-02-06 00:00:00
abstract：:A survey of U.S. stem cell scientists shows that uncertainty following the legal challenge to the Obama Administration's hESC research policy has negative scientific and economic impacts and affects a range of stem cell scientists, not just those working with hESCs. The international implications of these results are ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2011.01.002
更新日期：2011-02-04 00:00:00
abstract：:Regeneration of skin and hair follicles after wounding--a process known as wound-induced hair neogenesis (WIHN)--is a rare example of adult organogenesis in mammals. As such, WIHN provides a unique model system for deciphering mechanisms underlying mammalian regeneration. Here, we show that dsRNA, which is released fr...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2015.07.008
更新日期：2015-08-06 00:00:00
abstract：:Modifications of mRNAs can have a profound effect on cellular function and differentiation. In this issue of Cell Stem Cell, Batista et al. (2014) describe fundamental parameters of N(6)-methyl-adenosine modification of mRNAs in embryonic stem cells and provide strong evidence that modification plays a role in exit fr...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2014.11.011
更新日期：2014-12-04 00:00:00
abstract：:The Adenomatous Polyposis Coli (APC) gene is mutated in the majority of colorectal cancers (CRCs). Loss of APC leads to constitutively active WNT signaling, hyperproliferation, and tumorigenesis. Identification of pathways that facilitate tumorigenesis after APC loss is important for therapeutic development. Here, we ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2013.04.006
更新日期：2013-06-06 00:00:00
abstract：:Chronic liver injury can cause cirrhosis and impaired liver regeneration, impairing organ function. Adult livers can regenerate in response to parenchymal insults, and multiple cellular sources have been reported to contribute to this response. In this study, we modeled human chronic liver injuries, in which such resp...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2018.05.022
更新日期：2018-07-05 00:00:00
abstract：:All animals must excrete the waste products of metabolism. Excretion is performed by the kidney in vertebrates and by the Malpighian tubules in Drosophila. The mammalian kidney has an inherent ability for recovery and regeneration after ischemic injury. Stem cells and progenitor cells have been proposed to be responsi...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2007.07.003
更新日期：2007-08-16 00:00:00
abstract：:Dysfunction and loss of retinal pigment epithelium (RPE) leads to degeneration of photoreceptors in age-related macular degeneration and subtypes of retinitis pigmentosa. Human embryonic stem cells (hESCs) may serve as an unlimited source of RPE cells for transplantation in these blinding conditions. Here we show the ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2009.07.002
更新日期：2009-10-02 00:00:00
abstract：:Functionally relevant markers of glioblastoma stem-like cells (GSCs) have potential for therapeutic targeting to treat this aggressive disease. Here we used generation and screening of thousands of monoclonal antibodies to search for receptors and signaling pathways preferentially enriched in GSCs. We identified integ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2017.04.009
更新日期：2017-07-06 00:00:00
abstract：:Ezh2, the methyltransferase within Polycomb Repressive II Complexes, was thought to be essential for all H3K27me3 marks in embryonic stem cells (ESCs). Recently in Molecular Cell, Shen and colleagues (2008) revealed that EZH2 is dispensable for ESC derivation and self-renewal, and that EZH1 may unexpectedly compensate...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2008.11.007
更新日期：2008-12-04 00:00:00
