Naive versus Primed: It's Now Three-Dimensional.

Abstract:

:We are only beginning to understand the higher-order chromatin structure of pluripotent stem cells and its relevance to cell fate. Three recent studies used different approaches to reconstruct the 3D chromatin landscape of naive and primed pluripotent cells, unveiling common features as well as differences between these two states.

journal_name

Cell Stem Cell

journal_title

Cell stem cell

authors

Wei Z,Lu W

doi

10.1016/j.stem.2016.01.014

subject

Has Abstract

pub_date

2016-02-04 00:00:00

pages

164-5

issue

2

eissn

1934-5909

issn

1875-9777

pii

S1934-5909(16)00015-1

journal_volume

18

pub_type

评论,杂志文章
  • Advances in Zika Virus Research: Stem Cell Models, Challenges, and Opportunities.

    abstract::The re-emergence of Zika virus (ZIKV) and its suspected link with various disorders in newborns and adults led the World Health Organization to declare a global health emergency. In response, the stem cell field quickly established platforms for modeling ZIKV exposure using human pluripotent stem cell-derived neural p...

    journal_title:Cell stem cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.stem.2016.11.014

    authors: Ming GL,Tang H,Song H

    更新日期:2016-12-01 00:00:00

  • GFAP Mutations in Astrocytes Impair Oligodendrocyte Progenitor Proliferation and Myelination in an hiPSC Model of Alexander Disease.

    abstract::Alexander disease (AxD) is a leukodystrophy that primarily affects astrocytes and is caused by mutations in the astrocytic filament gene GFAP. While astrocytes are thought to have important roles in controlling myelination, AxD animal models do not recapitulate critical myelination phenotypes and it is therefore not c...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2018.07.009

    authors: Li L,Tian E,Chen X,Chao J,Klein J,Qu Q,Sun G,Sun G,Huang Y,Warden CD,Ye P,Feng L,Li X,Cui Q,Sultan A,Douvaras P,Fossati V,Sanjana NE,Riggs AD,Shi Y

    更新日期:2018-08-02 00:00:00

  • Self-Organizing 3D Human Trunk Neuromuscular Organoids.

    abstract::Neuromuscular networks assemble during early human embryonic development and are essential for the control of body movement. Previous neuromuscular junction modeling efforts using human pluripotent stem cells (hPSCs) generated either spinal cord neurons or skeletal muscles in monolayer culture. Here, we use hPSC-deriv...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2019.12.007

    authors: Faustino Martins JM,Fischer C,Urzi A,Vidal R,Kunz S,Ruffault PL,Kabuss L,Hube I,Gazzerro E,Birchmeier C,Spuler S,Sauer S,Gouti M

    更新日期:2020-02-06 00:00:00

  • Integration of genome-wide approaches identifies lncRNAs of adult neural stem cells and their progeny in vivo.

    abstract::Long noncoding RNAs (lncRNAs) have been described in cell lines and various whole tissues, but lncRNA analysis of development in vivo is limited. Here, we comprehensively analyze lncRNA expression for the adult mouse subventricular zone neural stem cell lineage. We utilize complementary genome-wide techniques includin...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2013.03.003

    authors: Ramos AD,Diaz A,Nellore A,Delgado RN,Park KY,Gonzales-Roybal G,Oldham MC,Song JS,Lim DA

    更新日期:2013-05-02 00:00:00

  • Capturing Totipotent Stem Cells.

    abstract::Minority subpopulations within embryonic stem cell cultures display an expanded developmental potential similar to that of early embryo blastomeres or the early inner cell mass. The ability to isolate and culture totipotent cells capable of giving rise to the entire conceptus would enhance our capacity to study early ...

    journal_title:Cell stem cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.stem.2017.12.011

    authors: Baker CL,Pera MF

    更新日期:2018-01-04 00:00:00

  • Pluripotent stem cells from cloned human embryos: success at long last.

    abstract::Recently in Cell, Mitalipov and colleagues report an advance that has eluded scientists for over a decade-the successful derivation of embryonic stem cell lines using somatic cell nuclear transfer, or SCNT (Tachibana et al., 2013). ...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2013.05.022

    authors: Trounson A,DeWitt ND

    更新日期:2013-06-06 00:00:00

  • Distinguishing mast cell and granulocyte differentiation at the single-cell level.

    abstract::The lineage restriction of prospectively isolated hematopoietic progenitors has been traditionally assessed by bulk in vitro culture and transplantation of large number of cells in vivo. These methods, however, cannot distinguish between homogenous multipotent or heterogeneous lineage-restricted populations. Using clo...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2010.02.013

    authors: Franco CB,Chen CC,Drukker M,Weissman IL,Galli SJ

    更新日期:2010-04-02 00:00:00

  • Chronic Liver Injury Induces Conversion of Biliary Epithelial Cells into Hepatocytes.

    abstract::Chronic liver injury can cause cirrhosis and impaired liver regeneration, impairing organ function. Adult livers can regenerate in response to parenchymal insults, and multiple cellular sources have been reported to contribute to this response. In this study, we modeled human chronic liver injuries, in which such resp...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2018.05.022

    authors: Deng X,Zhang X,Li W,Feng RX,Li L,Yi GR,Zhang XN,Yin C,Yu HY,Zhang JP,Lu B,Hui L,Xie WF

    更新日期:2018-07-05 00:00:00

  • Direct Reprogramming of RESTing Astrocytes.

    abstract::Understanding the mechanisms underlying neuronal fate determination will provide important insights into brain development and regenerative approaches to neurological diseases. Now in Cell Stem Cell, Masserdotti et al. (2015) use neuronal conversion of astrocytes to dissect transcriptional mechanisms of fate determina...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2015.06.011

    authors: Wang C,Fong H,Huang Y

    更新日期:2015-07-02 00:00:00

  • Growth signaling at the nexus of stem cell life and death.

    abstract::Stress can activate tumor-suppressive mechanisms, causing the loss of adult stem cell function with age. In this issue of Cell Stem Cell and in Nature, Castilho et al. (2009) and Harrison et al. (2009) highlight the importance of mTOR signaling in stem cell exhaustion and mammalian aging, respectively. ...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2009.08.008

    authors: Wood KC,Sabatini DM

    更新日期:2009-09-04 00:00:00

  • From Skin to Brain: A Parkinson's Disease Patient Transplanted with His Own Cells.

    abstract::In a pioneering study in New England Journal of Medicine, Schweitzer et al. (2020) report on a patient with Parkinson's disease who received a graft of dopamine neurons obtained from in vitro differentiated induced pluripotent stem cells, derived from the patient's own skin fibroblasts, showing the feasibility of auto...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2020.06.008

    authors: Parmar M,Björklund A

    更新日期:2020-07-02 00:00:00

  • Stem cells in 2009.

    abstract::There is intense contemporary interest in the identity, stability, and potential of stem cells, in the body or in the lab. The unusually comprehensive view provided by the 7th annual meeting of the ISSCR provides a framework to summarize recent progress. ...

    journal_title:Cell stem cell

    pub_type:

    doi:10.1016/j.stem.2009.10.012

    authors: McKay RD,van der Kooy D,Zwaka TP,Lin H

    更新日期:2009-11-06 00:00:00

  • RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions.

    abstract::RNA-binding proteins (RBPs), in addition to their functions in cellular homeostasis, play important roles in lineage specification and maintaining cellular identity. Despite their diverse and essential functions, which touch on nearly all aspects of RNA metabolism, the roles of RBPs in somatic cell reprogramming are p...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2016.12.002

    authors: Li H,Lai P,Jia J,Song Y,Xia Q,Huang K,He N,Ping W,Chen J,Yang Z,Li J,Yao M,Dong X,Zhao J,Hou C,Esteban MA,Gao S,Pei D,Hutchins AP,Yao H

    更新日期:2017-04-06 00:00:00

  • Looking Back: Cancer Stem Cells.

    abstract::Over the past decade, our growing understanding of cancer stem cell characteristics and function continues to guide innovative new treatments to eradicate cancer. For our tenth anniversary, we asked authors who cited a selection of our most popular papers in this area about the broad influences these studies have had....

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2017.05.005

    authors:

    更新日期:2017-06-01 00:00:00

  • Mitochondrial Shapeshifting Impacts AML Stemness and Differentiation.

    abstract::In this issue of Cell Stem Cell, Pei et al. (2018) demonstrate that leukemic stem cells (LSCs) have enhanced mitochondrial fission, which is positively regulated by FIS1. FIS1 is necessary to maintain LSC function; thus, targeting its expression and mitochondrial fission is a novel approach to decrease stemness and in...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2018.05.026

    authors: Schimmer AD

    更新日期:2018-07-05 00:00:00

  • Regenerative Rehabilitation: Applied Biophysics Meets Stem Cell Therapeutics.

    abstract::The emerging field of regenerative rehabilitation integrates biological and bioengineering advances in regenerative medicine with rehabilitative sciences. Here we highlight recent stem cell-based examples of the regenerative rehabilitation paradigm to promote tissue repair and regeneration, and we discuss remaining ch...

    journal_title:Cell stem cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.stem.2018.02.003

    authors: Rando TA,Ambrosio F

    更新日期:2018-03-01 00:00:00

  • Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro.

    abstract::Despite widespread interest in using human induced pluripotent stem cells (hiPSCs) in neurological disease modeling, a suitable model system to study human neuronal connectivity is lacking. Here, we report a comprehensive and efficient differentiation paradigm for hiPSCs that generate multiple CA3 pyramidal neuron sub...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2018.04.009

    authors: Sarkar A,Mei A,Paquola ACM,Stern S,Bardy C,Klug JR,Kim S,Neshat N,Kim HJ,Ku M,Shokhirev MN,Adamowicz DH,Marchetto MC,Jappelli R,Erwin JA,Padmanabhan K,Shtrahman M,Jin X,Gage FH

    更新日期:2018-05-03 00:00:00

  • TGF-β Pathway Inhibition Signals New Hope for Fanconi Anemia.

    abstract::An improved understanding of the bone marrow failure (BMF) mechanisms in Fanconi anemia (FA) may improve current therapeutic strategies. Zhang et al. identify hyperactive TGF-β signaling as an underlying cause of BMF in FA mice and patient cells, whose inhibition promotes DNA repair and hematopoietic stem and progenit...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2016.04.008

    authors: Tummala H,Dokal I

    更新日期:2016-05-05 00:00:00

  • Out of Many, One: Computational Reconstruction of Mouse Skin using Single-Cell Transcriptomics.

    abstract::Mammalian skin is a complex and heterogeneous tissue with several distinct compartments and stem cell populations. Joost et al. (2016) now use single-cell RNaseq to comprehensively reconstruct this complexity, revealing spatial and pseudotemporal differences between transcriptional programs in distinct compartments an...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2016.09.009

    authors: Chen JC,Christiano AM

    更新日期:2016-10-06 00:00:00

  • The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

    abstract::Cardiac stem/progenitor cells hold great potential for regenerative therapies; however, the mechanisms regulating their expansion and differentiation remain insufficiently defined. Here we show that Ldb1 is a central regulator of genome organization in cardiac progenitor cells, which is crucial for cardiac lineage dif...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2015.08.007

    authors: Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

    更新日期:2015-09-03 00:00:00

  • Quo vadis brain repair? A long axonal journey in the adult CNS.

    abstract::Recently in Nature Neuroscience, Gaillard et al. (2007) study axonal projections from embryonic cortical explants grafted into acutely damaged adult motor cortex. After attempting to rule out fusion of donor tissue with pre-existing host circuitry, the authors report robust long-distance donor axonal projections and s...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2007.09.012

    authors: Singec I,Snyder EY

    更新日期:2007-10-11 00:00:00

  • Venetolax with Azacitidine Drains Fuel from AML Stem Cells.

    abstract::In Nature Medicine, Pollyea et al. (2018) recently reported a remarkable overall response of newly diagnosed elderly acute myeloid leukemia patients to a venetoclax and azacitidine combination in a new clinical trial. This treatment reduced succinate dehydrogenase glutathionylation, impaired the tricarboxylic acid cyc...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2018.12.005

    authors: Nakada D

    更新日期:2019-01-03 00:00:00

  • Primed for pluripotency.

    abstract::To date, pluripotent epiblast stem cells (EpiSCs) had only been derived from postimplantation mouse embryos. In this issue of Cell Stem Cell, Najm et al. (2011) demonstrate that EpiSCs can be routinely derived from preimplantation embryos, showing that both human and mouse blastocysts can produce the same class of pri...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2011.02.009

    authors: Kunath T

    更新日期:2011-03-04 00:00:00

  • Directed differentiation of human embryonic stem cells into functional retinal pigment epithelium cells.

    abstract::Dysfunction and loss of retinal pigment epithelium (RPE) leads to degeneration of photoreceptors in age-related macular degeneration and subtypes of retinitis pigmentosa. Human embryonic stem cells (hESCs) may serve as an unlimited source of RPE cells for transplantation in these blinding conditions. Here we show the ...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2009.07.002

    authors: Idelson M,Alper R,Obolensky A,Ben-Shushan E,Hemo I,Yachimovich-Cohen N,Khaner H,Smith Y,Wiser O,Gropp M,Cohen MA,Even-Ram S,Berman-Zaken Y,Matzrafi L,Rechavi G,Banin E,Reubinoff B

    更新日期:2009-10-02 00:00:00

  • FLT3-ITD knockin impairs hematopoietic stem cell quiescence/homeostasis, leading to myeloproliferative neoplasm.

    abstract::Internal tandem duplication (ITD) mutations within the FMS-like tyrosine kinase-3 (FLT3) render the receptor constitutively active driving proliferation and survival in leukemic blasts. Expression of FLT3-ITD from the endogenous promoter in a murine knockin model results in progenitor expansion and a myeloproliferativ...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2012.05.027

    authors: Chu SH,Heiser D,Li L,Kaplan I,Collector M,Huso D,Sharkis SJ,Civin C,Small D

    更新日期:2012-09-07 00:00:00

  • Dramatic Expansion of HSCs: New Possibilities for HSC Transplants?

    abstract::The identification of mechanisms that regulate stem cell renewal has led to the development of innovative approaches to expand hematopoietic stem cells ex vivo. One approach reported by Wagner et al. (2016) in this issue of Cell Stem Cell has demonstrated dramatic expansion of umbilical cord blood stem cells that prom...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2015.12.011

    authors: Nikiforow S,Ritz J

    更新日期:2016-01-07 00:00:00

  • Inhibition of Aberrant DNA Re-methylation Improves Post-implantation Development of Somatic Cell Nuclear Transfer Embryos.

    abstract::Somatic cell nuclear transfer (SCNT) enables cloning of differentiated cells by reprogramming their nuclei to a totipotent state. However, successful full-term development of SCNT embryos is a low-efficiency process and arrested embryos frequently exhibit epigenetic abnormalities. Here, we generated genome-wide DNA me...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2018.07.017

    authors: Gao R,Wang C,Gao Y,Xiu W,Chen J,Kou X,Zhao Y,Liao Y,Bai D,Qiao Z,Yang L,Wang M,Zang R,Liu X,Jia Y,Li Y,Zhang Y,Yin J,Wang H,Wan X,Liu W,Zhang Y,Gao S

    更新日期:2018-09-06 00:00:00

  • Gut with the Program: Direct Reprogramming toward Intestinal Epithelium Realized.

    abstract::Intestinal organoids offer great promise for modeling intestinal diseases; however, harvesting intestinal tissue is invasive and directed hPSC differentiation protocols are laborious and costly. In this issue of Cell Stem Cell, Miura and Suzuki (2017) describe the direct conversion of somatic cells from both mice and ...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2017.09.011

    authors: Nakauka-Ddamba A,Lengner CJ

    更新日期:2017-10-05 00:00:00

  • Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

    abstract::Innate immune factors may restrict hematopoietic stem cell (HSC) genetic engineering and contribute to broad individual variability in gene therapy outcomes. Here, we show that HSCs harbor an early, constitutively active innate immune block to lentiviral transduction that can be efficiently overcome by cyclosporine H ...

    journal_title:Cell stem cell

    pub_type: 杂志文章

    doi:10.1016/j.stem.2018.10.008

    authors: Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

    更新日期:2018-12-06 00:00:00

  • Collisions on the Busy DNA Highway Set Up Barriers for Reprogramming.

    abstract::Why most cells remain refractory to transcription factor (TF)-induced fate conversion remains largely mysterious, with the answers holding important instructions on how to effectively direct cell identities. In this issue of Cell Stem Cell, Babos et al. (2019) show that conflicts caused by simultaneous high transcript...

    journal_title:Cell stem cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.stem.2019.09.007

    authors: Hu X,Guo S

    更新日期:2019-10-03 00:00:00