Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs.

abstract：:Most disease variants lie within noncoding genomic regions, making their functional interpretation challenging. Because chromatin openness strongly influences transcriptional activity, we hypothesized that cell-type-specific open chromatin regions (OCRs) might highlight disease-relevant noncoding sequences. To investi...

journal_title：Cell stem cell

authors： Forrest MP,Zhang H,Moy W,McGowan H,Leites C,Dionisio LE,Xu Z,Shi J,Sanders AR,Greenleaf WJ,Cowan CA,Pang ZP,Gejman PV,Penzes P,Duan J

更新日期：2017-09-07 00:00:00

abstract：:How DNA methylation inhibitors exert their anticancer effects in patients is not well understood. In the latest issue of Cancer Cell, Tsai et al. (2012) use low-dose drug treatment to induce persistent attenuation of tumorigenicity by targeting tumor-initiating cells. ...

journal_title：Cell stem cell

authors： Shen H,Laird PW

更新日期：2012-04-06 00:00:00

abstract：:Mouse embryonic stem cells (mESCs) cultured under serum/LIF conditions exhibit heterogeneous expression of pluripotency-associated factors that can be overcome by two inhibitors (2i) of the MEK and GSK3 pathways. Several studies have shown that the "ground state" induced by 2i is characterized by global hypomethylatio...

journal_title：Cell stem cell

authors： Galonska C,Ziller MJ,Karnik R,Meissner A

更新日期：2015-10-01 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT) enables cloning of differentiated cells by reprogramming their nuclei to a totipotent state. However, successful full-term development of SCNT embryos is a low-efficiency process and arrested embryos frequently exhibit epigenetic abnormalities. Here, we generated genome-wide DNA me...

journal_title：Cell stem cell

authors： Gao R,Wang C,Gao Y,Xiu W,Chen J,Kou X,Zhao Y,Liao Y,Bai D,Qiao Z,Yang L,Wang M,Zang R,Liu X,Jia Y,Li Y,Zhang Y,Yin J,Wang H,Wan X,Liu W,Zhang Y,Gao S

更新日期：2018-09-06 00:00:00

abstract：:Recently in Cell, Mitalipov and colleagues report an advance that has eluded scientists for over a decade-the successful derivation of embryonic stem cell lines using somatic cell nuclear transfer, or SCNT (Tachibana et al., 2013). ...

journal_title：Cell stem cell

authors： Trounson A,DeWitt ND

更新日期：2013-06-06 00:00:00

abstract：:The California Institute for Regenerative Medicine (CIRM) Alpha Stem Cell Clinic (ASCC) Network was launched in 2015 to address a compelling unmet medical need for rigorous, FDA-regulated, stem cell-related clinical trials for patients with challenging, incurable diseases. Here, we describe our multi-center experience...

journal_title：Cell stem cell

authors： Jamieson CHM,Millan MT,Creasey AA,Lomax G,Donohoe ME,Walters MC,Abedi M,Bota DA,Zaia JA,Adams JS

更新日期：2018-06-01 00:00:00

abstract：:Internal tandem duplication (ITD) mutations within the FMS-like tyrosine kinase-3 (FLT3) render the receptor constitutively active driving proliferation and survival in leukemic blasts. Expression of FLT3-ITD from the endogenous promoter in a murine knockin model results in progenitor expansion and a myeloproliferativ...

journal_title：Cell stem cell

authors： Chu SH,Heiser D,Li L,Kaplan I,Collector M,Huso D,Sharkis SJ,Civin C,Small D

更新日期：2012-09-07 00:00:00

abstract：:Human induced pluripotent stem cells (hiPSCs) hold promise for myocardial repair following injury, but preclinical studies in large animal models are required to determine optimal cell preparation and delivery strategies to maximize functional benefits and to evaluate safety. Here, we utilized a porcine model of acute...

journal_title：Cell stem cell

authors： Ye L,Chang YH,Xiong Q,Zhang P,Zhang L,Somasundaram P,Lepley M,Swingen C,Su L,Wendel JS,Guo J,Jang A,Rosenbush D,Greder L,Dutton JR,Zhang J,Kamp TJ,Kaufman DS,Ge Y,Zhang J

更新日期：2014-12-04 00:00:00

abstract：:Trophoblast stem cells (TSCs) arise from the first cell fate decision in the developing embryo and generate extra-embryonic lineages, giving rise to the fetal portion of the placenta. Mouse embryonic and extra-embryonic lineages are strictly separated by a distinct epigenetic barrier, which is not fully overcome follo...

journal_title：Cell stem cell

authors： Kubaczka C,Senner CE,Cierlitza M,Araúzo-Bravo MJ,Kuckenberg P,Peitz M,Hemberger M,Schorle H

更新日期：2015-11-05 00:00:00

abstract：:In the mouse embryo, the aorta-gonad-mesonephros (AGM) region is considered to be the sole location for intraembryonic emergence of hematopoietic stem cells (HSCs). Here we report that, in parallel to the AGM region, the E10.5-E11.5 mouse head harbors bona fide HSCs, as defined by long-term, high-level, multilineage r...

journal_title：Cell stem cell

authors： Li Z,Lan Y,He W,Chen D,Wang J,Zhou F,Wang Y,Sun H,Chen X,Xu C,Li S,Pang Y,Zhang G,Yang L,Zhu L,Fan M,Shang A,Ju Z,Luo L,Ding Y,Guo W,Yuan W,Yang X,Liu B

更新日期：2012-11-02 00:00:00

abstract：:Combination of stem cell-based approaches with gene-editing technologies represents an attractive strategy for studying human disease and developing therapies. However, gene-editing methodologies described to date for human cells suffer from technical limitations including limited target gene size, low targeting effic...

journal_title：Cell stem cell

authors： Liu GH,Suzuki K,Qu J,Sancho-Martinez I,Yi F,Li M,Kumar S,Nivet E,Kim J,Soligalla RD,Dubova I,Goebl A,Plongthongkum N,Fung HL,Zhang K,Loring JF,Laurent LC,Izpisua Belmonte JC

更新日期：2011-06-03 00:00:00

abstract：:The kidney's complex spatial organization and poorly defined lineage specification programs have impeded derivation of kidney progenitors from pluripotent stem cells (PSCs). Now in Cell Stem Cell, Taguchi et al. (2014) redefine the identity of embryonic kidney progenitors in vivo to obtain PSC-derived kidney progenito...

journal_title：Cell stem cell

authors： Gouon-Evans V

更新日期：2014-01-02 00:00:00

abstract：:DNA hydroxylation catalyzed by Tet dioxygenases occurs abundantly in embryonic stem cells and neurons in mammals. However, its biological function in vivo is largely unknown. Here, we demonstrate that Tet1 plays an important role in regulating neural progenitor cell proliferation in adult mouse brain. Mice lacking Tet...

journal_title：Cell stem cell

authors： Zhang RR,Cui QY,Murai K,Lim YC,Smith ZD,Jin S,Ye P,Rosa L,Lee YK,Wu HP,Liu W,Xu ZM,Yang L,Ding YQ,Tang F,Meissner A,Ding C,Shi Y,Xu GL

更新日期：2013-08-01 00:00:00

abstract：:In this study, we explore endoderm cell fate regulation through the expression of lineage-determining transcription factors. We demonstrate that stable endoderm progenitors can be established from human ES cells by constitutive expression of SOX7 or SOX17, producing extraembryonic endoderm and definitive endoderm prog...

journal_title：Cell stem cell

authors： Séguin CA,Draper JS,Nagy A,Rossant J

更新日期：2008-08-07 00:00:00

abstract：:The study of MSC trafficking is clinically relevant for minimally invasive cell therapy to promote regeneration of damaged tissue, to treat inflammation, and to promote angiogenesis. However, these studies are complicated by the diverse methods used to culture, characterize, and deliver MSCs and by the variety of meth...

journal_title：Cell stem cell

authors： Karp JM,Leng Teo GS

更新日期：2009-03-06 00:00:00

abstract：:Somatic cells can be induced into pluripotent stem cells (iPSCs) with a combination of four transcription factors, Oct4/Sox2/Klf4/c-Myc or Oct4/Sox2/Nanog/LIN28. This provides an enabling platform to obtain patient-specific cells for various therapeutic and research applications. However, several problems remain for t...

journal_title：Cell stem cell

authors： Shi Y,Desponts C,Do JT,Hahm HS,Schöler HR,Ding S

更新日期：2008-11-06 00:00:00

abstract：:Leukemia stem cells (LSCs) are resistant to current therapies used to treat chronic myeloid leukemia (CML). Abraham et al. (2016) have identified a molecular network critical for CML LSC survival and propose that simultaneously targeting two of their major transcriptional regulators, p53 and c-Myc, may facilitate thei...

journal_title：Cell stem cell

authors： Carvajal LA,Steidl U

更新日期：2016-07-07 00:00:00

abstract：:Gastric cancer displays marked molecular heterogeneity with aggressive behavior and treatment resistance. Therefore, good in vitro models that encompass unique subtypes are urgently needed for precision medicine development. Here, we have established a primary gastric cancer organoid (GCO) biobank that comprises norma...

journal_title：Cell stem cell

authors： Yan HHN,Siu HC,Law S,Ho SL,Yue SSK,Tsui WY,Chan D,Chan AS,Ma S,Lam KO,Bartfeld S,Man AHY,Lee BCH,Chan ASY,Wong JWH,Cheng PSW,Chan AKW,Zhang J,Shi J,Fan X,Kwong DLW,Mak TW,Yuen ST,Clevers H,Leung SY

更新日期：2018-12-06 00:00:00

abstract：:Although the clinical outcomes of cell therapy trials have not met initial expectations, emerging evidence suggests that injury-mediated tissue damage might benefit from the delivery of cells or their secreted products. Pluripotent stem cells (PSCs) are promising cell sources primarily because of their capacity to gen...

journal_title：Cell stem cell

authors： Desgres M,Menasché P

更新日期：2019-11-07 00:00:00

abstract：:Knowledge of both the global chromatin structure and the gene expression programs of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) should provide a robust means to assess whether the genomes of these cells have similar pluripotent states. Recent studies have suggested that ESCs and iPSCs...

journal_title：Cell stem cell

authors： Guenther MG,Frampton GM,Soldner F,Hockemeyer D,Mitalipova M,Jaenisch R,Young RA

更新日期：2010-08-06 00:00:00

abstract：:Sox family transcription factors have emerged as key regulators of multiple developmental processes, including fate determination and differentiation in multiple tissues. In this issue of Cell Stem Cell, Nowak et al. (2008) implicate Sox9 in the specification of early hair follicle stem cells and subsequent epithelial...

journal_title：Cell stem cell

authors： Christiano AM

更新日期：2008-07-03 00:00:00

abstract：:To prevent insertional mutagenesis arising from retroviral reactivation, cells of embryonic origin possess a unique capacity to silence retroviruses. Given the distinct modes of X chromosome inactivation between embryonic and extraembryonic lineages, we investigated paradigms of viral extinction. We show that trophect...

journal_title：Cell stem cell

authors： Golding MC,Zhang L,Mann MR

更新日期：2010-05-07 00:00:00

abstract：:Cellular reprogramming converts differentiated cells into induced pluripotent stem cells (iPSCs). However, this process is typically very inefficient, complicating mechanistic studies. We identified and molecularly characterized rare, early intermediates poised to reprogram with up to 95% efficiency, without perturbin...

journal_title：Cell stem cell

authors： Schwarz BA,Cetinbas M,Clement K,Walsh RM,Cheloufi S,Gu H,Langkabel J,Kamiya A,Schorle H,Meissner A,Sadreyev RI,Hochedlinger K

更新日期：2018-08-02 00:00:00

abstract：:In a recent issue of Nature, Tachibana et al. (2009) conducted chromosomal transfer into recipient primate oocytes to yield live offspring or monkey ESCs with no contribution of donor mitochondria. The success of this technique may increase demand for human oocyte donation for research. ...

journal_title：Cell stem cell

authors： Cibelli J

更新日期：2009-10-02 00:00:00

abstract：:Rapid progress has recently been made regarding how the niche controls stem cell function, but little is yet known about how stem cells in the same niche interact with one another. In this study, we show that differentiation-defective Drosophila ovarian germline stem cells (GSCs) can outcompete normal ones for niche o...

journal_title：Cell stem cell

authors： Jin Z,Kirilly D,Weng C,Kawase E,Song X,Smith S,Schwartz J,Xie T

更新日期：2008-01-10 00:00:00

abstract：:Cerebral organoids represent a new model in which to study human brain development outside the human body. Recently in Nature Biotechnology, Lancaster et al. (2017) tackled the lack of reproducibility, tissue homogeneity, and complexity of this system by bioengineering organoids to establish the next generation of hum...

journal_title：Cell stem cell

authors： Koch P,Ladewig J

更新日期：2017-08-03 00:00:00

abstract：:Modifications of mRNAs can have a profound effect on cellular function and differentiation. In this issue of Cell Stem Cell, Batista et al. (2014) describe fundamental parameters of N(6)-methyl-adenosine modification of mRNAs in embryonic stem cells and provide strong evidence that modification plays a role in exit fr...

journal_title：Cell stem cell

authors： Jalkanen AL,Wilusz J

更新日期：2014-12-04 00:00:00

abstract：:Acute Myelogenous Leukemia (AML) is an aggressive cancer that strikes both adults and children and is frequently resistant to therapy. Thus, identifying signals needed for AML propagation is a critical step toward developing new approaches for treating this disease. Here, we show that Tetraspanin 3 is a target of the ...

journal_title：Cell stem cell

authors： Kwon HY,Bajaj J,Ito T,Blevins A,Konuma T,Weeks J,Lytle NK,Koechlein CS,Rizzieri D,Chuah C,Oehler VG,Sasik R,Hardiman G,Reya T

更新日期：2015-08-06 00:00:00

abstract：:The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficientl...

journal_title：Cell stem cell

authors： Tang H,Hammack C,Ogden SC,Wen Z,Qian X,Li Y,Yao B,Shin J,Zhang F,Lee EM,Christian KM,Didier RA,Jin P,Song H,Ming GL

更新日期：2016-05-05 00:00:00

abstract：:Substantial expansion in the number of cerebral cortex neurons is thought to underlie cognitive differences between humans and other primates, although the mechanisms underlying this expansion are unclear. Otani et al. (2016) utilize PSC-derived brain organoids to study how species-specific differences in cortical pro...

journal_title：Cell stem cell

authors： Dyer MA

更新日期：2016-04-07 00:00:00