Abstract:
:Recurrent mutations in cohesin complex proteins have been identified in pre-leukemic hematopoietic stem cells and during the early development of acute myeloid leukemia and other myeloid malignancies. Although cohesins are involved in chromosome separation and DNA damage repair, cohesin complex functions during hematopoiesis and leukemic development are unclear. Here, we show that mutant cohesin proteins block differentiation of human hematopoietic stem and progenitor cells (HSPCs) in vitro and in vivo and enforce stem cell programs. These effects are restricted to immature HSPC populations, where cohesin mutants show increased chromatin accessibility and likelihood of transcription factor binding site occupancy by HSPC regulators including ERG, GATA2, and RUNX1, as measured by ATAC-seq and ChIP-seq. Epistasis experiments show that silencing these transcription factors rescues the differentiation block caused by cohesin mutants. Together, these results show that mutant cohesins impair HSPC differentiation by controlling chromatin accessibility and transcription factor activity, possibly contributing to leukemic disease.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Mazumdar C,Shen Y,Xavy S,Zhao F,Reinisch A,Li R,Corces MR,Flynn RA,Buenrostro JD,Chan SM,Thomas D,Koenig JL,Hong WJ,Chang HY,Majeti Rdoi
10.1016/j.stem.2015.09.017subject
Has Abstractpub_date
2015-12-03 00:00:00pages
675-688issue
6eissn
1934-5909issn
1875-9777pii
S1934-5909(15)00424-5journal_volume
17pub_type
杂志文章相关文献
Cell Stem Cell文献大全abstract::Similarities between basal cell carcinoma (BCC) tumor cells and hair follicle keratinocytes had previously suggested that BCC originates within the hair follicle bulge stem cell niche. However, in the current Nature Cell Biology, Youssef et al. (2010) show that BCC instead originates in the interfollicular epidermis. ...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2010.03.009
更新日期:2010-04-02 00:00:00
abstract::Ectopic expression of combinations of transcription factors (TFs) can drive direct lineage conversion, thereby reprogramming a somatic cell's identity. To determine the molecular mechanisms by which Gata4, Mef2c, and Tbx5 (GMT) induce conversion from a cardiac fibroblast toward an induced cardiomyocyte, we performed c...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2019.06.012
更新日期:2019-07-03 00:00:00
abstract::Two recent papers outline improvements in gene editing technology that may facilitate the analysis of signaling networks important for development. The systems developed by both Thyagarajan and coworkers (Thyagarajan et al., 2007) in Nature Biotechnology, and Lombardo and colleagues (Lombardo et al., 2007) in Stem Cel...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2007.11.004
更新日期:2007-12-13 00:00:00
abstract::As a response against serious infection, there is often a need to produce more leukocytes to defend the organism against the infectious agent. In this issue of Cell Stem Cell, Goodell and colleagues (Feng et al., 2008) find a link between regulators of host defense and hematopoietic stem cells. ...
journal_title:Cell stem cell
pub_type: 评论,社论
doi:10.1016/j.stem.2007.12.002
更新日期:2008-01-10 00:00:00
abstract::DNA methylation is an epigenetic mark that plays pivotal roles in gene regulation, but its functions in neural fate decisions are poorly understood. In this issue of Cell Stem Cell, Ziller et al. (2018) show that the de novo methyltransferase Dnmt3a ensures efficient generation of motor neurons from stem cells. ...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2018.03.006
更新日期:2018-04-05 00:00:00
abstract::Large cutaneous and non-healing ulcers exhibit impaired epithelialization, which represents a major therapeutic challenge. Recently in Nature, Kurita et al. (2018) reported a surprising potential source of epithelial cells. The authors demonstrate that wound-resident mesenchymal cells can be reprogrammed to generate e...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2018.10.014
更新日期:2018-11-01 00:00:00
abstract::A survey of U.S. stem cell scientists shows that uncertainty following the legal challenge to the Obama Administration's hESC research policy has negative scientific and economic impacts and affects a range of stem cell scientists, not just those working with hESCs. The international implications of these results are ...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2011.01.002
更新日期:2011-02-04 00:00:00
abstract::Accessibility of human oocytes for research poses a serious ethical challenge to society. This fact categorically holds true when pursuing some of the most promising areas of research, such as somatic cell nuclear transfer and embryonic stem cell studies. One approach to overcoming this limitation is to use an oocyte ...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2007.10.009
更新日期:2007-11-01 00:00:00
abstract::Stem cell-based therapies for Parkinson's disease are moving into a new and exciting era, with several groups pursuing clinical trials with pluripotent stem cell (PSC)-derived dopamine neurons. As many groups have ongoing or completed GMP-level cell manufacturing, we highlight key clinical translation considerations f...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.09.014
更新日期:2017-11-02 00:00:00
abstract::Two independent studies by Li et al. (2010) and Samavarchi-Tehrani et al. (2010) in this issue of Cell Stem Cell suggest that a mesenchymal-to-epithelial transition is a critical initiating event during the derivation of induced pluripotent stem cells (iPSCs) from fibroblasts, indicating remarkable similarities betwee...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2010.05.018
更新日期:2010-07-02 00:00:00
abstract::In injured tissues, regeneration is often associated with cell fate plasticity, in that cells deviate from their normal lineage paths. It is becoming increasingly clear that this plasticity often creates alternative strategies to restore damaged or lost cells. Alternatively, cell fate plasticity is also part and parce...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2018.05.009
更新日期:2018-09-06 00:00:00
abstract::The identification of tumor-initiating cells represents a significant challenge for studies of prostate cancer. In a recent issue of Cell Reports, Agarwal and colleagues use organoid culture to ascertain two distinct luminal progenitors in a mouse model of prostate cancer, shedding new light on lineage relationships i...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2015.11.018
更新日期:2015-12-03 00:00:00
abstract::The re-emergence of Zika virus (ZIKV) and its suspected link with various disorders in newborns and adults led the World Health Organization to declare a global health emergency. In response, the stem cell field quickly established platforms for modeling ZIKV exposure using human pluripotent stem cell-derived neural p...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2016.11.014
更新日期:2016-12-01 00:00:00
abstract::Aging is characterized by a decrease in genome integrity, impaired organ maintenance, and an increased risk of cancer, which coincide with clonal dominance of expanded mutant stem and progenitor cell populations in aging tissues, such as the intestinal epithelium, the hematopoietic system, and the male germline. Here ...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2015.05.002
更新日期:2015-06-04 00:00:00
abstract::Achieving immune tolerance through cell transplantation is a promising approach for treating autoimmune disease. In this issue of Cell Stem Cell, Akiyama et al. (2012) demonstrate that human and mouse mesenchymal stem cells can induce immune suppression by attracting and killing autoreactive T cells, which stimulates ...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2012.04.013
更新日期:2012-05-04 00:00:00
abstract::Human induced pluripotent stem cells (HiPSCs) appear to be highly similar to human embryonic stem cells (HESCs). Using two genetic lineage-tracing systems, we demonstrate the generation of iPSC lines from human pancreatic islet beta cells. These reprogrammed cells acquired markers of pluripotent cells and differentiat...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2011.06.007
更新日期:2011-07-08 00:00:00
abstract::Animal cloning can be achieved through somatic cell nuclear transfer (SCNT), although the live birth rate is relatively low. Recent studies have identified H3K9me3 in donor cells and abnormal Xist activation as epigenetic barriers that impede SCNT. Here we overcome these barriers using a combination of Xist knockout d...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.06.008
更新日期:2018-09-06 00:00:00
abstract::The cellular identity of niche cells that regulate hematopoietic stem cell (HSC) self-renewal and differentiation has been debated for several years. Two recent studies in Nature (Ding and Morrison, 2013; Greenbaum et al., 2013) have shed light into the bone marrow stromal subsets making CXCL12, a chemokine critical f...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2013.03.012
更新日期:2013-04-04 00:00:00
abstract::Emerging evidence strongly suggests that stem cells and their differentiated progeny display distinct metabolic profiles, but how metabolic changes are coupled with organogenesis has remained unclear. Homem et al. (2014) now reveal a hormone-dependent pathway that couples metabolic changes with stem cell differentiati...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2014.08.006
更新日期:2014-09-04 00:00:00
abstract::Muscle stem cell regenerative capacity is rapidly lost during ex vivo culture. In this issue of Cell Stem Cell, Judson et al. (2018) show that inhibition of cytoplasmic SETD7, a lysine methyltransferase, potently expands naive, undifferentiated mouse and human muscle stem cells by restricting their progression through...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2018.01.007
更新日期:2018-02-01 00:00:00
abstract::Adult neurogenesis regulates plasticity and function in the hippocampus, which is critical for memory and vulnerable to Alzheimer's disease (AD). Promoting neurogenesis may improve hippocampal function in AD brains. However, how amyloid beta (Abeta), the key AD pathogen, affects the development and function of adult-b...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2009.10.003
更新日期:2009-12-04 00:00:00
abstract::Individuals with neurofibromatosis type 1 (NF1) develop abnormalities of both neuronal and glial cell lineages, suggesting that the NF1 protein neurofibromin is an essential regulator of neuroglial progenitor function. In this regard, Nf1-deficient embryonic telencephalic neurospheres exhibit increased self-renewal an...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2007.07.008
更新日期:2007-10-11 00:00:00
abstract::Cellular reprogramming converts differentiated cells into induced pluripotent stem cells (iPSCs). However, this process is typically very inefficient, complicating mechanistic studies. We identified and molecularly characterized rare, early intermediates poised to reprogram with up to 95% efficiency, without perturbin...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.06.013
更新日期:2018-08-02 00:00:00
abstract::Mammalian skin is a complex and heterogeneous tissue with several distinct compartments and stem cell populations. Joost et al. (2016) now use single-cell RNaseq to comprehensively reconstruct this complexity, revealing spatial and pseudotemporal differences between transcriptional programs in distinct compartments an...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2016.09.009
更新日期:2016-10-06 00:00:00
abstract::Hematopoietic stem cells (HSCs) sustain hematopoiesis throughout life. HSCs exit dormancy to restore hemostasis in response to stressful events, such as acute blood loss, and must return to a quiescent state to prevent their exhaustion and resulting bone marrow failure. HSC activation is driven in part through the pho...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.05.003
更新日期:2018-06-01 00:00:00
abstract::Myeloid-biased hematopoietic stem cells (MB-HSCs) play critical roles in recovery from injury, but little is known about how they are regulated within the bone marrow niche. Here we describe an auto-/paracrine physiologic circuit that controls quiescence of MB-HSCs and hematopoietic progenitors marked by histidine dec...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.11.003
更新日期:2017-12-07 00:00:00
abstract::Polycomb group (PcG) proteins are essential regulators of stem cells. PcG and trithorax group proteins mark developmental regulator gene promoters with bivalent domains consisting of overlapping repressive and activating histone modifications to keep them poised for activation in embryonic stem cells. Bmi1, a componen...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2010.01.005
更新日期:2010-03-05 00:00:00
abstract::Most disease variants lie within noncoding genomic regions, making their functional interpretation challenging. Because chromatin openness strongly influences transcriptional activity, we hypothesized that cell-type-specific open chromatin regions (OCRs) might highlight disease-relevant noncoding sequences. To investi...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.07.008
更新日期:2017-09-07 00:00:00
abstract::Prognostic markers that capture leukemia stem cell (LSC) activity can be useful for the risk stratification of acute myeloid leukemia (AML) patients. In a recent issue of Nature, Ng et al. (2016) develop a prognostic score based on a 17-gene expression signature of LSCs to predict outcome in AML patients. ...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.02.010
更新日期:2017-03-02 00:00:00
abstract::Recent studies have shown that tissue-specific stem cells (SCs) found throughout the body respond differentially to DNA damage. In this review, we will discuss how different SC populations sense and functionally respond to DNA damage, identify various common and distinct mechanisms utilized by tissue-specific SCs to a...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2010.12.012
更新日期:2011-01-07 00:00:00