Abstract：
:To assess the genetic consequences of induced pluripotent stem cell (iPSC) reprogramming, we sequenced the genomes of ten murine iPSC clones derived from three independent reprogramming experiments, and compared them to their parental cell genomes. We detected hundreds of single nucleotide variants (SNVs) in every clone, with an average of 11 in coding regions. In two experiments, all SNVs were unique for each clone and did not cluster in pathways, but in the third, all four iPSC clones contained 157 shared genetic variants, which could also be detected in rare cells (<1 in 500) within the parental MEF pool. These data suggest that most of the genetic variation in iPSC clones is not caused by reprogramming per se, but is rather a consequence of cloning individual cells, which "captures" their mutational history. These findings have implications for the development and therapeutic use of cells that are reprogrammed by any method.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Young MA,Larson DE,Sun CW,George DR,Ding L,Miller CA,Lin L,Pawlik KM,Chen K,Fan X,Schmidt H,Kalicki-Veizer J,Cook LL,Swift GW,Demeter RT,Wendl MC,Sands MS,Mardis ER,Wilson RK,Townes TM,Ley TJdoi
10.1016/j.stem.2012.03.002subject
Has Abstractpub_date
2012-05-04 00:00:00pages
570-82issue
5eissn
1934-5909issn
1875-9777pii
S1934-5909(12)00119-1journal_volume
10pub_type
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