Abstract：
:Developing technologies for efficient and scalable disruption of gene expression will provide powerful tools for studying gene function, developmental pathways, and disease mechanisms. Here, we develop clustered regularly interspaced short palindromic repeat interference (CRISPRi) to repress gene expression in human induced pluripotent stem cells (iPSCs). CRISPRi, in which a doxycycline-inducible deactivated Cas9 is fused to a KRAB repression domain, can specifically and reversibly inhibit gene expression in iPSCs and iPSC-derived cardiac progenitors, cardiomyocytes, and T lymphocytes. This gene repression system is tunable and has the potential to silence single alleles. Compared with CRISPR nuclease (CRISPRn), CRISPRi gene repression is more efficient and homogenous across cell populations. The CRISPRi system in iPSCs provides a powerful platform to perform genome-scale screens in a wide range of iPSC-derived cell types, dissect developmental pathways, and model disease.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Mandegar MA,Huebsch N,Frolov EB,Shin E,Truong A,Olvera MP,Chan AH,Miyaoka Y,Holmes K,Spencer CI,Judge LM,Gordon DE,Eskildsen TV,Villalta JE,Horlbeck MA,Gilbert LA,Krogan NJ,Sheikh SP,Weissman JS,Qi LS,So PL,Conkdoi
10.1016/j.stem.2016.01.022subject
Has Abstractpub_date
2016-04-07 00:00:00pages
541-53issue
4eissn
1934-5909issn
1875-9777pii
S1934-5909(16)00023-0journal_volume
18pub_type
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