Abstract：
AIMS:Maternal type 2 diabetes (T2D) can result in adverse pathological outcomes to both the mother and fetus. The present study aimed to investigate the pathological effects of maternal T2D on the gene expression patterns and functions of fetal human umbilical vein endothelial cells (HUVECs), a representative of fetal vascular cells. METHODS:Cell proliferation, apoptosis, mitochondrial ROS production and cell cycle were measured using flowcytometry. Genome-wide expression was measured using Affymetrix microarray. Gene expression of CCND2, STAT1, ITGB8, ALDH2, and ADAMTS5 was measured using real-time PCR. RESULTS:HUVECs derived from T2D mothers (T2D-HUVECs) showed elevated levels of mitochondrial superoxide anions, reduced cell proliferation, and increased apoptosis rates relative to HUVECs derived from healthy control mothers (C.HUVECs). In addition , T2D-HUVECs showed a decreased proportion of cells in G0/G1 and cell cycle arrest at the S phases relative to controls. Interestingly, microarray experiments revealed significant differences in genome-wide expression profiles between T2D-HUVECs and C.HUVECs. In particular, the analysis identified 90 upregulated genes and 42 downregulated genes. The upregulated genes CCND2, STAT1, ITGB8, ALDH2, and ADAMTS5 were validated as potential biomarkers for fetal endothelial dysfunction. Functional network analysis revealed that these genes are the important players that participate in the pathogenesis of endothelial dysfunction, which in turn influences the inflammatory response, cellular movement, and cardiovascular system development and function. CONCLUSION:Sustained alterations in the overall function of T2D-HUVEC and gene expression profiles provided insights into the role of maternal T2D on the pathophysiology of the fetal endothelial dysfunction.
journal_name
Acta Diabetoljournal_title
Acta diabetologicaauthors
Sultan Sdoi
10.1007/s00592-018-1207-ysubject
Has Abstractpub_date
2019-01-01 00:00:00pages
73-85issue
1eissn
0940-5429issn
1432-5233pii
10.1007/s00592-018-1207-yjournal_volume
56pub_type
杂志文章abstract：:In the present study, we investigated whether the ameliorating effect of aminoguanidine on diabetes-related proteinuria and nephropathy is associated with glomerular basement membrane heparan sulphate contents. STZ-induced diabetic rats developed proteinuria (at the tenth week: diabetic rats, 713 +/- 418 mg protein pe...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s005920070022
更新日期：2000-01-01 00:00:00
abstract：:The original report on the microencapsulation of islets of Langerhans used sodium alginate and poly-L-lysine (PLL) to form the capsules. Although several alternative materials have subsequently been used with vary-mg degrees of success, it is those studies using islets encapsulated in alginate-PLL-alginate which are r...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/BF00569928
更新日期：1993-01-01 00:00:00
abstract：:Serum gamma-glutamyl transferase (gamma-GT), a marker of oxidative stress, predicts morbidity and mortality from cardiovascular disease. Plasma total homocysteine (tHcy), a pro-oxidant and also an independent risk factor for cardiovascular disease, correlates with gamma-GT among some populations. It is not known wheth...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0001-z
更新日期：2007-12-01 00:00:00
abstract：:The possible occurrence of increased non-enzymatic glycosylation of serum insulin-like growth factor binding protein-3 (IGFBP-3) in vivo and the changes that would simultaneously occur in serum levels of IGFBP-3 and insulin-like growth factor-1 (IGF-I) were investigated. We measured levels of IGF-I and IGFBP-3 and the...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s005920050109
更新日期：1998-07-01 00:00:00
abstract：:Diabetes cardiomyopathy is a specific form of cardiac disease characteristic for diabetic patients. Development of echocardiography enabled diagnosis of diabetic cardiomyopathy significantly before the occurrence of heart failure. Previously was believed that left ventricular (LV) diastolic dysfunction represents the ...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-020-01528-2
更新日期：2020-09-01 00:00:00
abstract：AIMS:This study aimed to assess islet insulin secretion and insulin resistance in Chinese patients with latent autoimmune diabetes in adults (LADA). METHODS:Twelve patients with LADA, 10 with type 1 diabetes mellitus (T1DM), 10 with type 2 diabetes mellitus (T2DM), and 10 nondiabetic healthy controls (HCs) were includ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-019-01387-6
更新日期：2019-11-01 00:00:00
abstract：:Although the effects of sex, ageing, height, race, and current and past health on pulmonary function tests have been described, only non-significant associations have been observed between body weight and lung function among healthy persons after having accounted for the effects of age and height. However, few studies...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-003-0023-0
更新日期：2003-10-01 00:00:00
abstract：:We investigated whether food supplementation within the first year life or age at introduction of gluten-containing foods influenced the risk of developing islet autoimmunity and type 1 diabetes. A total of 2,291 children with a family history of type 1 diabetes were prospectively followed from birth for 28,983 patien...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0628-5
更新日期：2015-06-01 00:00:00
abstract：:Patients with diabetes represent an increasing proportion of end-stage renal disease (ESRD) patients. Cardiovascular risk, already formidable among patients on dialysis, is significantly higher among those who also have diabetes. Diabetic ESRD patients are not only at higher risk of ischaemic events, but are also subj...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s005920200003
更新日期：2002-04-01 00:00:00
abstract：:We assessed the efficacy of noninsulin antidiabetic medications used in current clinical practice (metformin, sulfonylureas, α-glucosidase inhibitors, thiazolidinediones, glinides, dipeptidyl peptidase-4 inhibitors, glucagon-like peptide-1 agonists) to reach the HbA1c target <7% in people with type 2 diabetes. MEDLINE...
journal_title：Acta diabetologica
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00592-012-0370-9
更新日期：2014-04-01 00:00:00
abstract：:The aim of this study was to compare the efficacy of treatment with insulin alone, insulin plus acarbose, insulin plus metformin, or insulin plus rosiglitazone in type 2 diabetic subjects who were previously on insulin monotherapy, and to evaluate the effects of these treatments on cardiovascular risk factors includin...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-007-0004-9
更新日期：2007-12-01 00:00:00
abstract：:Endothelial nitric oxide synthase (eNOS) has been shown to play an essential role in retinal vascular function, and disequilibrium in its production can lead to diabetic retinopathy (DR). Genetic polymorphisms of eNOS gene have been suggested to play a role in nitric oxide (NO) abnormalities which may contribute to th...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-012-0437-7
更新日期：2012-12-01 00:00:00
abstract：:In the perfused pancreas of adult rats that were injected with streptozotocin (STZ) during the neonatal period, the release of insulin caused by glucose is more severely affected than that evoked by other secretagogues. We have now examined whether a comparable situation prevails in vivo. In anesthetised rats, the 0-1...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00570366
更新日期：1994-09-01 00:00:00
abstract：AIMS:Familial partial lipodystrophy (FPLD) is a rare autosomal dominant disorder, mostly due to mutations in lamin A (LMNA) or in peroxisome proliferator-activated receptor gamma (PPARG) genes. In the present study, we aimed to identify and functionally characterize the genetic defect underlying FPLD in an Italian fami...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-019-01462-y
更新日期：2020-05-01 00:00:00
abstract：AIMS:Type 2 diabetes (DM2) is associated to oxidative modifications of high-density lipoproteins (HDL), which can interfere with their function. Pioglitazone has proved effective in raising HDL cholesterol (HDL-C) and lowering small dense low-density lipoprotein (LDL), but no clinical studies have examined its effect o...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-018-01278-2
更新日期：2019-05-01 00:00:00
abstract：:We report the results of bone mineral density (BMD) measurements in type 2 diabetic patients, in comparison to healthy controls. In this prospective study, a total of 277 subjects (aged 30-60 years) with type 2 diabetes mellitus, outpatients at the Cukurova Medical School Hospital, were evaluated for BMD at L(1)-L(4) ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s005920300014
更新日期：2003-06-01 00:00:00
abstract：:The renin-angiotensin system (RAS) has an important role in the endocrine pancreas. Multiple researches have shown that even in the insulin resistance phase, there are many abnormalities in islets morphology and function. This study aimed at investigating the effects of RAS blockade on the islets function in rats with...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-010-0210-8
更新日期：2013-08-01 00:00:00
abstract：:Although reactive oxygen species (ROS) contribute to glucose intolerance induced by the renin-angiotensin system (RAS) is well documented, the role of the newly discovered pathway of RAS, angiotensin (Ang)-(1-7)/Mas axis, in this process remains unknown. Here, we examined the effect of Ang-(1-7) on oxidative stress an...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-011-0348-z
更新日期：2012-08-01 00:00:00
abstract：AIMS:To study the incidence of and the factors associated with renal dialysis and transplantation in type 1 (T1DM) and type 2 diabetes (T2DM). METHODS:Data on individuals who had received dialysis treatment or renal transplant between 1 January 2004 and 31 December 2013 were extracted from the regional administrative ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-018-1142-y
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:Circulating levels of TNF alpha receptor 1 (TNFR1) and 2 (TNFR2) are associated with increased long-term mortality and impaired kidney function. AIM:To study association between circulating levels of TNFR1 and TNFR2 and short-term mortality in patients with diabetes and dyspnea. POPULATION AND METHODS:Pati...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-020-01527-3
更新日期：2020-10-01 00:00:00
abstract：AIMS:Fat accumulation in the liver and in the muscle results in hepatic and muscle insulin resistance and has been associated with increased cardiovascular risk. It is unclear whether the individual role of hepatic and muscle insulin resistance in the onset of dyslipidaemia is observed in nonalcoholic fatty liver disea...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-015-0816-y
更新日期：2016-06-01 00:00:00
abstract：:Genetic linkage studies of families with early-onset type 2 diabetes have facilitated the identification of diabetes-susceptibility genes. In order to assess the feasibility of using linkage approaches to identify genes responsible for the development of type 2 diabetes in Japanese subjects, we examined our clinical r...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00581039
更新日期：1995-03-01 00:00:00
abstract：:This study examined whether or not changes in plasma concentrations of motilin and other gastrointestinal hormones known to affect gastric motility are associated with the accelerated gastric emptying seen during hypoglycaemia. While studying gastric emptying by scintigraphy in eight healthy subjects, the plasma conce...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s005920050073
更新日期：1997-10-01 00:00:00
abstract：:Autoantibodies to zinc transporter 8 (ZnT8A) are a powerful diagnostic or predictive marker in type 1 diabetes. However, the widely used current ZnT8A radioligand binding assay (RBA) has proved to be difficult for many laboratories to implement. The aim of this study was the development and characterization of the per...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-013-0532-4
更新日期：2014-01-01 00:00:00
abstract：:This study was aimed at the assessment of incidence of malignancies in type 2 diabetic patients treated with different sulphonylureas. A matched case-control study was performed. Cases were 195 diabetic patients aged 69.0 +/- 9.2 years who had an incident malignancy. Controls were 195 diabetic patients, unaffected by ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-008-0083-2
更新日期：2009-12-01 00:00:00
abstract：:Aim of this study was to formulate an index for glucose effectiveness (Sg), SgIo, based on 3-point (0, 30 and 120 min) 75 g oral glucose tolerance test (OGTT). The equation for SgI(O) was developed in the Chikuma cohort (n = 502). Firstly, post-loading plasma glucose without insulin action and Sg (PPG-without insulin ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-012-0417-y
更新日期：2012-12-01 00:00:00
abstract：AIMS:Although diabetes is rapidly increasing in India, there is no national consensus on best practices for screening, diagnosis, and management of gestational diabetes mellitus (GDM). The goal of this study was to systematically review the literature for studies reporting the prevalence and screening and diagnostic me...
journal_title：Acta diabetologica
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00592-018-1131-1
更新日期：2018-06-01 00:00:00
abstract：:The aim of this study was to evaluate the prevalence of thyroid autoantibodies and clinical significance of thyroid autoimmunity in children with the diagnosis of diabetes mellitus type 1. The study group comprised 222 children all with newly diagnosed diabetes mellitus type 1 (122 boys) with a mean age 9.92 +/- 4.5 y...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0017-4
更新日期：2008-03-01 00:00:00
abstract：:Insulin resistance is a major factor in the pathogenesis of type 2 diabetes mellitus (T2DM) and is related to the fatty acid profile of the plasma membranes. The purpose of the present study was to investigate fatty acid composition and cholesterol content of cell membranes in patients with type 2 diabetes and, thus, ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0224-4
更新日期：2006-12-01 00:00:00
abstract：:Insulin secretion is stimulated better by oral than by intravenous glucose (incretin effect). The contribution of the autonomic nervous system to the incretin effect after oral glucose in humans is unclear. We therefore examined nine type 1 diabetic (insulin-dependent) patients with end-stage nephropathy, studied afte...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00572873
更新日期：1993-01-01 00:00:00
