Repair of base-base mismatches in simian and human cells.

Abstract:

:Mismatched heteroduplexes arise as intermediates of several dissimilar genetic processes. The outcome of these genetic events will therefore be influenced by the efficiency and specificity of mismatch repair. We have studied the correction of base-base mispairs in simian and human fibroblasts by transfecting the cells with derivatives of SV40 DNA, each harboring a single mispair in a defined orientation. Analysis of plaques revealed that correction efficiencies for homomispairs followed the pattern G.G greater than C.C greater than or equal to A.A greater than T.T. Repair bias was influenced by flanking sequences. Correction efficiences for heteromispairs followed the pattern of G.T greater than A.C greater than C.T greater than A.G and repair favored the retention of G + C by a substantial margin. This repair specificity could lead to a gene conversion bias favoring the accumulation of G + C in sequences subject to high levels of recombination or unequal exchange.

journal_name

Genome

journal_title

Genome

authors

Brown TC,Jiricny J

doi

10.1139/g89-107

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

578-83

issue

2

eissn

0831-2796

issn

1480-3321

journal_volume

31

pub_type

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