Abstract:
:Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of soybean (Glycine max (L.) Merr.) in North America, since it was first found in the Midwest of the United States in 2000. Biotypes of the soybean aphid capable of colonizing newly developed aphid-resistant soybean cultivars have been recently discovered. Genetic resources, including molecular markers, to study soybean aphids are severely lacking. Recently developed next generation sequencing platforms offer opportunities for high-throughput and inexpensive genome sequencing and rapid marker development. The objectives of this study were (i) to develop and characterize genomic SSR markers from soybean aphid genomic sequences generated by next generation sequencing technology and (ii) to evaluate the utility of the SSRs for genetic diversity or relationship analyses. In total 128 SSR primer pairs were designed from sequences generated by Illumina GAII from a reduced representation library of A. glycines. Nearly 94% (120) of the primer pairs amplified SSR alleles of expected size and 24 SSR loci were polymorphic among three aphid samples from three populations. The polymorphic SSRs were successfully used to differentiate among 24 soybean aphids from Ohio and South Dakota. Sequencing of PCR products of two SSR markers from four aphid samples revealed that the allelic polymorphism was due to variation in the SSR repeats among the aphids. These markers should be particularly useful for genetic differentiation among aphids collected from soybean fields at different localities and regions. These SSR markers provide the soybean aphid research community with the first set of PCR-based codominant markers developed from the genomic sequences of A. glycines.
journal_name
Genomejournal_title
Genomeauthors
Jun TH,Michel AP,Mian MAdoi
10.1139/g11-002subject
Has Abstractpub_date
2011-05-01 00:00:00pages
360-7issue
5eissn
0831-2796issn
1480-3321journal_volume
54pub_type
杂志文章相关文献
GENOME文献大全abstract::Yams are species of the genus Dioscorea (family Dioscoreaceae), which consists of approximately 630 species. The majority of the world production of yams occurs in Africa with 58.8 million t annually, but they are also produced in the Americas and Asia. The saponins in yams have been reported to possess various proper...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0105
更新日期:2017-03-01 00:00:00
abstract::Quantitative trait loci (QTL) controlling traits associated with winterhardiness in barley (field survival, LT50, growth habit, and crown fructan content) were mapped to chromosome 7 in a population of 100 F1-derived doubled haploid lines. The largest QTL effects for all traits were detected in a 21% recombination int...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-009
更新日期:1993-02-01 00:00:00
abstract::The nearly complete mitochondrial (mt) genome of an egg parasitoid, Trissolcus basalis (Wollaston), was sequenced using both 454 and Illumina next-generation sequencing technologies. A portion of the noncoding region remained unsequenced, possibly owing to the presence of repeats. The sequenced portion of the genome i...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-005
更新日期:2012-03-01 00:00:00
abstract::Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We hav...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-015
更新日期:2010-05-01 00:00:00
abstract::In order to map the genes conditioning the induction of embryos during our anther culture process, we evaluated F2 plants from three different crosses for their anther culture ability and also performed RFLP analysis on these plants. The results showed that six chromosomal regions appear to be associated with the abil...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-127
更新日期:1995-10-01 00:00:00
abstract::Segregation patterns of polymorphic simple sequence repeat (SSR) primer pairs were investigated in monoploid potato families derived from anther culture. A total of 14 primers developed from the sequences in the database, as well as from a genomic library of potato, was used. Distorted segregation was observed for sev...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-140
更新日期:2002-04-01 00:00:00
abstract::Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, is one of the most important foliar diseases of barley (Hordeum vulgare L.) in North America. The primary problem caused by this disease is substantial yield loss. The objective of this study was to determine the chromosomal location of SSLB resistan...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-032
更新日期:2010-08-01 00:00:00
abstract::MicroRNAs (miRNAs) repress target genes at the post-transcriptional level and play important roles in development and cell lineage decisions. In vertebrates, however, both the targets of miRNAs and their expression profiles during development are poorly understood. Thus far, 326 human miRNAs, 249 mouse miRNAs, and 195...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-120
更新日期:2006-10-01 00:00:00
abstract::The combination of two divergent genomes during hybridization can result in "genome shock". Although genome shock has been reported in the hybrids of some herbaceous plants, the pattern and the principle it follows are far from understood, especially in woody plants. Here, the gene expression patterns were remodeled i...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0105
更新日期:2018-07-01 00:00:00
abstract::Fusarium head blight (FHB) is one of the most important fungal wheat diseases worldwide. Understanding the genetics of FHB resistance is the key to facilitating the introgression of different FHB resistance genes into adapted wheat. The objectives of the present study were to detect and map quantitative trait loci (QT...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-104
更新日期:2005-04-01 00:00:00
abstract::Allopolyploids undergo 'genomic shock' leading to large genetic and epigenetic modifications. Previous studies mainly focused on nuclear changes, while little is known about the inheritance and changes of organelle genome in allopolyploidization. The synthetic allotetraploid Cucumis ×hytivus, which is generated via hy...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0134
更新日期:2021-01-18 00:00:00
abstract::We have used immunocytochemistry and molecular cloning methods to identify and characterize structural polypeptides of the centromere. These studies permit us to resolve two distinct regions: the inner and outer centromere. (i) Components of the outer centromere: autoantibodies from certain patients with rheumatic dis...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/g89-103
更新日期:1989-01-01 00:00:00
abstract::Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obta...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-077
更新日期:1997-10-01 00:00:00
abstract::Canada wild rye (CWR, Elymus canadensis L., 2n = 4x = 28) is a potential source of genes for disease resistance and environmental tolerance in barley (Hordeum vulgare L., 2n = 2x = 14). Tissue cultures were initiated from immature inflorescences of CWR x 'Betzes' barley hybrids to promote CWR introgression into barley...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-047
更新日期:1996-04-01 00:00:00
abstract::Meiosis was studied in two diploid (2n = 2x = 24) siblings of Solanum phureja Juz. et Buk. and in 11 disomic and 2 trisomic descendants. The diploid siblings carry the same heterozygous interchange and either one or two inversions. The frequency of quadrivalents at diakinesis/metaphase I in these clones was 0.56 and 0...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-017
更新日期:1995-02-01 00:00:00
abstract::Twelve genes encoding two closely related subtypes (ST1.1a and ST1.1b) of a sulphate transporter have been identified in the diploid wheats Aegilops tauschii, Triticum urartu, and Aegilops speltoides, as well as the hexaploid Triticum aestivum. Based on phylogenetic comparisons with other plant sulphate transporters, ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-011
更新日期:2004-06-01 00:00:00
abstract::GATA-GACA repetitive sequences first isolated from a female snake (termed BKm sequences) and associated with sex chromosomes in some species were hybridized to DNA from rainbow trout (Salmo gairdneri). Genomic DNA was studied from three groups of rainbow trout: (i) randomly selected males and females from an outbred g...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g89-523
更新日期:1989-10-01 00:00:00
abstract::Disease lesion mimics provide an excellent biological system to study the genetic basis of cell death in plants. Many lesion mimics show variation in phenotype expression in different genetic backgrounds. Our goal was to identify quantitative trait loci (QTL) modifying lesion mimic expression thereby identifying genet...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-046
更新日期:2004-10-01 00:00:00
abstract::A F2 population of two celery cultivated types (Apium graveolens L. var. rapaceum and A. graveolens L. var. secalinum) was used to construct a linkage map consisting of 29 RFLP (restriction fragment length polymorphism), 100 RAPD (random amplified polymorphic DNA), four isozyme, one disease resistance, and one growth ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-005
更新日期:1995-02-01 00:00:00
abstract::Being an essential mineral nutrient, potassium (K+) plays numerous important roles in plant growth and development and determines the yield and quality of crop products. The cellular level of K+ is controlled to a large extent by the K+ transporter, which belongs to the KT/HAK/KUP (HAK) family. However, little is know...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0187
更新日期:2019-04-01 00:00:00
abstract::Anther development in angiosperms culminates in the programmed cell death of specific tissues to facilitate the release of pollen. Despite a wealth of morphological descriptions of this process, there have been few reports on the regulation of dehiscence or the coordination of events between tissues. We have cloned an...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Selection of wheat germplasm for a range of quality traits has been a challenging exercise because of the cost of testing, the variation within testing data, and a poor understanding of the underlying genetics. The objective of this study was to identify quantitative trait loci (QTLs) underlying quality traits in whea...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-045
更新日期:2009-08-01 00:00:00
abstract::Mismatched heteroduplexes arise as intermediates of several dissimilar genetic processes. The outcome of these genetic events will therefore be influenced by the efficiency and specificity of mismatch repair. We have studied the correction of base-base mispairs in simian and human fibroblasts by transfecting the cells...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g89-107
更新日期:1989-01-01 00:00:00
abstract::Changes in chromosome number have played an important role in the evolution of the genus Trifolium. Along with a few species of polyploid origin there are several cases of dysploidy as evidenced by the presence of four basic chromosome numbers (x = 8, 7, 6, 5). Trifolium subterraneum and Trifolium israeliticum are rel...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2013-0055
更新日期:2013-06-01 00:00:00
abstract::A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshif...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-042
更新日期:2005-10-01 00:00:00
abstract::The length variability of the nontranscribed spacer (NTS) of the 5S rDNA repeats was analyzed in species of the genus Lens by means of PCR amplification. The NTS ranged from approximately 227 to approximately 952 bp. The polymorphism detected was higher than previous NTS polymorphisms described in this genus. Three NT...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-052
更新日期:2005-10-01 00:00:00
abstract::Fluorescent in situ hybridization (FISH) of a 130 kilobase cotton (Gossypium hirsuitum L.) bacterial artificial chromosome (BAC) clone containing a high proportion of single-copy DNA produced a large pair of FISH signals on the distal end of the long arm of a pair of chromosomes of the D-genome species G. raimondii Ul...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-082
更新日期:1995-08-01 00:00:00
abstract::Unisexual Ambystoma are the oldest known unisexual vertebrates and comprise a lineage of eastern North American all female salamanders that reproduce by stealing sperm from as many as five normally bisexual congeneric species. The sperm may be used to only stimulate egg development by gynogenesis but can be incorporat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2019-0034
更新日期:2019-08-01 00:00:00
abstract::Five wheat-barley translocations in a wheat background were characterized through the combination of cytogenetic and molecular genetic approaches. The wheat chromosome segments involved in the translocations were identified using sequential GISH and two-colour FISH with the probes pSc119.2 and pAs1. The barley chromat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-068
更新日期:2002-12-01 00:00:00
abstract::A new repetitive sequence that is extremely abundant and well dispersed in the Thinopyrum elongatum genome but present in low-copy number in wheat (Triticum aestivum L.) has been isolated. This repeat and a Th. elongatum repeat isolated in another laboratory were used to identify cosmid genomic clones containing the r...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-012
更新日期:1994-02-01 00:00:00