Visualizing DNA domains and sequences by microscopy: a fifty-year history of molecular cytogenetics.

abstract：:In this paper we analyze genetically and cytologically a Y-chromosome 2 translocation with several markers, some of which are potentially useful for large scale sex separation. The breakpoint of this Y-2 translocation is located at region 6B on the trichogene polytene chromosome map. It is found that, in strains carry...

journal_title：Genome

authors： Cladera JL,Delprat MA

更新日期：1995-12-01 00:00:00

abstract：:Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obta...

journal_title：Genome

authors： Pedersen C,Langridge P

更新日期：1997-10-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross b...

journal_title：Genome

authors： Ouédraogo JT,Tignegre JB,Timko MP,Beizile FJ

更新日期：2002-10-01 00:00:00

abstract：:5S rDNA loci have been mapped on barley chromosomes by in situ hybridization using five reciprocal translocation lines. Two kinds of DNA probes covering either the 5S rDNA coding region or the 5S rDNA coding and flanking noncoding regions were used. They were prepared by direct cloning from interphase nuclei and simul...

journal_title：Genome

authors： Fukui K,Kamisugi Y,Sakai F

更新日期：1994-02-01 00:00:00

abstract：:We have used nuclear and chloroplast molecular markers to genotype cultivated and wild accessions of Vitis vinifera L. from Tunisia and assess their genetic relationships. Fifty-five distinct genotypes were identified among 80 cultivated accessions, including 18 genotypic groups containing between 2 and 5 accessions p...

journal_title：Genome

authors： Snoussi H,Slimane MH,Ruiz-García L,Martínez-Zapater JM,Arroyo-García R

更新日期：2004-12-01 00:00:00

abstract：:The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capit...

journal_title：Genome

authors： Zhao JT,Frommer M,Sved JA,Zacharopoulou A

更新日期：1998-08-01 00:00:00

abstract：:The first doubled haploid oat linkage map constructed at MTT Agrifood Research Finland was supplemented with additional microsatellites and Diversity Array Technology (DArT) markers to produce a map containing 1058 DNA markers and 34 linkage groups. The map was used to locate quantitative trait loci (QTLs) for 11 impo...

journal_title：Genome

authors： Tanhuanpää P,Manninen O,Beattie A,Eckstein P,Scoles G,Rossnagel B,Kiviharju E

更新日期：2012-04-01 00:00:00

abstract：:Twenty-nine lines with deletions in the Lr19 ('Indis') translocated chromosome segment were used to physically map three Thinopyrum RFLP loci as well as the Sr25 and Sd1 loci. From the data, the relative locations of marker loci on the translocation were determined as: Sd1, Xpsr165, Xpsr105, Xps129, Lr19, Wsp-D1, Sr25...

journal_title：Genome

authors： Prins R,Marais GF,Marais AS,Janse BJ,Pretorius ZA

更新日期：1996-10-01 00:00:00

abstract：:Southern blots of restriction fragments of genomic DNAs from Hordeum vulgare (L.), H. bulbosum (L.), and interspecific hybrids and their derivatives were hybridized with rDNA probe to identify locus-specific modifications at Rrn loci. H. bulbosum rDNA revealed a single EcoRV site per repeat compared with two sites in ...

journal_title：Genome

authors： Kranthi Kumar P,Subrahmanyam NC

更新日期：1999-12-01 00:00:00

abstract：:High soil boron (B) constitutes a major soil problem in many parts of the world, particularly in low-rainfall areas and land under irrigation. Low accumulation of B in the shoot or grain of cereal crops is correlated with the maintenance of biomass production and grain yield under high B conditions, suggesting that th...

journal_title：Genome

authors： Schnurbusch T,Langridge P,Sutton T

更新日期：2008-12-01 00:00:00

abstract：:Using a complementary (c)DNA-amplified fragment length polymorphism (AFLP) approach, we investigated differential gene expression linked to resistance mechanisms during the incompatible potato - Globodera pallida interaction. Expression was compared between a resistant and a susceptible potato clone, inoculated or not...

journal_title：Genome

authors： Jolivet K,Grenier E,Bouchet JP,Esquibet M,Kerlan MC,Caromel B,Mugniéry D,Lefebvre V

更新日期：2007-04-01 00:00:00

abstract：:Microsatellites have many desirable marker properties and have been increasingly used in crop plants in genetic diversity studies. Here we report on the characterisation of microsatellite markers and on their use for the determination of genetic identities and the assessment of genetic variability among accessions fro...

journal_title：Genome

authors： Jakse J,Kindlhofer K,Javornik B

更新日期：2001-10-01 00:00:00

abstract：:The emergence of next generation sequencing has increased by several orders of magnitude the amount of data available for phylogenetics. Reduced representation approaches, such as restriction-sited associated DNA sequencing (RADseq), have proven useful for phylogenetic studies of non-model species at a wide range of p...

journal_title：Genome

authors： Fitz-Gibbon S,Hipp AL,Pham KK,Manos PS,Sork VL

更新日期：2017-09-01 00:00:00

abstract：:Mitochondrial (mt) genome sequences of insects are receiving renewed attention in molecular phylogentic studies, studies of mt-genome rearrangement, and other unusual molecular phenomena, such as translational frameshifting. At present, the basal neopteran lineages are poorly represented by mt-genome sequences. Comple...

journal_title：Genome

authors： Stewart JB,Beckenbach AT

更新日期：2006-07-01 00:00:00

abstract：:Polycelis nigra is a free-living simultaneous hermaphroditic flatworm that has amphimictic and pseudogamous parthenogenetic biotypes. Sexual individuals are always diploid (2n = 16) and pseudogamous parthenogens are polyploid (usually triploid). Two types of supernumerary chromosomes are found in parthenogens, those r...

journal_title：Genome

authors： Sharbel TF,Beukeboom LW,Pijnacker LP

更新日期：1997-12-01 00:00:00

abstract：:Sequence-based markers have added a new dimension in the efficiency of identifying alien introgressions in wheat. Expressed sequence tag-sequence tagged sites (EST-STS) markers have proved useful in tracing alien chromatin. In this study, we report the development of Thinopyrum bessarabicum- and Secale anatolicum-spec...

journal_title：Genome

authors： Singh AK,Lo K,Dong C,Zhang P,Trethowan RM,Sharp PJ

更新日期：2020-11-01 00:00:00

abstract：:Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well...

journal_title：Genome

authors： He C,Sayed-Tabatabaei BE,Komatsuda T

更新日期：2004-12-01 00:00:00

abstract：:Disease resistance gene candidates (RGCs) belonging to the nucleotide-binding site (NBS) superfamily have been cloned from numerous crop plants using highly conserved DNA sequence motifs. The aims of this research were to (i) isolate genomic DNA clones for RGCs in cultivated sunflower (Helianthus annuus L.) and (ii) m...

journal_title：Genome

authors： Gedil MA,Slabaugh MB,Berry S,Johnson R,Michelmore R,Miller J,Gulya T,Knapp SJ

更新日期：2001-04-01 00:00:00

abstract：:Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridizati...

journal_title：Genome

authors： Gao D,Schmidt T,Jung C

更新日期：2000-12-01 00:00:00

abstract：:A recent investigation, using a human genomic probe, has indicated that the 68,000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p less than 0.0001). In addition, we have fou...

journal_title：Genome

authors： Somerville MJ,McLachlan DR,Percy ME

更新日期：1988-08-01 00:00:00

abstract：:Multiple reaction monitoring mass spectrometry (MRM-MS) is an emerging technology for blood biomarker verification and validation; however, the results may be influenced by pre-analytical factors. This exploratory study was designed to determine if differences in phlebotomy techniques would significantly affect the ab...

journal_title：Genome

authors： Penn AM,Lu L,Chambers AG,Balshaw RF,Morrison JL,Votova K,Wood E,Smith DS,Lesperance M,del Zoppo GJ,Borchers CH,SpecTRA Study Group.

更新日期：2015-12-01 00:00:00

abstract：:The 5S rDNA repeat unit of the cockle Cerastoderma glaucum from the Mediterranean and Baltic coasts was PCR amplified and sequenced. The length of the units was 539-568 bp, of which 120 bp were assigned to the 5S rRNA gene and 419-448 bp to the spacer region, and the G/C content was 46%-49%, 54%, and 44%-47%, respecti...

journal_title：Genome

authors： Freire R,Insua A,Méndez J

更新日期：2005-06-01 00:00:00

abstract：:The octamer-binding transcription factor 4 gene (Oct4) plays a critical role in maintaining pluripotency during early mammalian embryonic development and self-renewal of embryonic stem (ES) cells. In this study, we cloned the Oct4 cDNA and 2.8-kb regulatory region upstream of the start codon in Guanzhong dairy goat ( ...

journal_title：Genome

authors： Cheng X,Meng S,Deng J,Lai W,Wang H

更新日期：2011-10-01 00:00:00

abstract：:We previously demonstrated that allopolyploidization could induce phenotypic variations and genome changes in a newly synthesized allotetraploid in Cucumis. To explore the molecular involvement of epigenetic phenomena, we investigated cytosine methylation in Cucumis by using methylation-sensitive amplified polymorphis...

journal_title：Genome

authors： Chen L,Chen J

更新日期：2008-10-01 00:00:00

abstract：:Expensive and unsustainable fishmeal is increasingly being replaced with cheaper lipids and carbohydrates as sources of energy in aquaculture. Although it is known that the excess of lipids and carbohydrates has negative effects on nutrient utilization, growth, metabolic homeostasis, and health of fish, our current un...

journal_title：Genome

authors： Prisingkorn W,Jakovlić I,Yi SK,Deng FY,Zhao YH,Wang WM

更新日期：2019-02-01 00:00:00

abstract：:A survey of restriction fragment polymorphism in mitochondrial DNA (mtDNA) of 17 populations of the mosquito Aedes albopictus was undertaken. The mtDNA size was estimated to be about 17.5 kbp. The level of polymorphism was low, with over 99% of the fragments being shared in common among the 17 populations. Three popul...

journal_title：Genome

authors： Kambhampati S,Rai KS

更新日期：1991-04-01 00:00:00

abstract：:The SRY gene is a single-copy, male-specific gene, located on the Y chromosome in most mammals. However, recently we have described the presence of multiple polymorphic copies of this gene in both males and females of the vole species Microtus cabrerae. Here, we present the chromosomal localization of SRY gene copies ...

journal_title：Genome

authors： Fernández R,Barragán MJ,Bullejos M,Marchal JA,Martínez S,Díaz de la Guardia R,Sánchez A

更新日期：2002-06-01 00:00:00

abstract：:Bacteria are essential components of natural environments. They contribute to ecosystem functioning through roles as mutualists and pathogens for larger species, and as key components of food webs and nutrient cycles. Bacterial communities respond to environmental disturbances, and the tracking of these communities ac...

journal_title：Genome

authors： Farrell MJ,Govender D,Hajibabaei M,van der Bank M,Davies TJ

更新日期：2019-03-01 00:00:00

abstract：:Complex chromosome rearrangements (CCRs) are defined as structural abnormalities involving more than two chromosome breaks, coupled with exchanges of chromosomal segments. Information on CCRs in plants is limited. In the present study, a plant (26-4) harboring translocation chromosomes 1RS.1BL and 4RS.4DL was selected...

journal_title：Genome

authors： Ren T,Li Z,Yan B,Tan F,Tang Z,Fu S,Yang M,Ren Z

更新日期：2016-12-01 00:00:00

abstract：:The objectives of the present research were to determine the effects of water stress on seed-quality traits and to map QTLs controlling the studied traits under two different water treatments in a population of sunflower recombinant inbred lines (RILs). Two experiments were conducted in greenhouse and field conditions...

journal_title：Genome

authors： Ebrahimi A,Maury P,Berger M,Kiani SP,Nabipour A,Shariati F,Grieu P,Sarrafi A

更新日期：2008-08-01 00:00:00