Identification of the entire chromosome complement of bread wheat by two-colour FISH.

abstract：:The timing and rate of genomic variation induced by allopolyploidization in the intergeneric wheat-rye (Triticum spp. - Secale cereale L.) hybrid triticale (x Triticosecale Wittmack) was studied using amplified fragment length polymorphism (AFLP) analyses with 2 sets of primers, EcoRI-MseI (E-M) and PstI-MseI (P-M), w...

journal_title：Genome

authors： Ma XF,Gustafson JP

更新日期：2006-08-01 00:00:00

abstract：:Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of...

journal_title：Genome

authors： Jun TH,Michel AP,Mian MA

更新日期：2011-05-01 00:00:00

abstract：:Five wheat-barley translocations in a wheat background were characterized through the combination of cytogenetic and molecular genetic approaches. The wheat chromosome segments involved in the translocations were identified using sequential GISH and two-colour FISH with the probes pSc119.2 and pAs1. The barley chromat...

journal_title：Genome

authors： Nagy ED,Molnár-Láng M,Linc G,Láng L

更新日期：2002-12-01 00:00:00

abstract：:Changes in chromosome number have played an important role in the evolution of the genus Trifolium. Along with a few species of polyploid origin there are several cases of dysploidy as evidenced by the presence of four basic chromosome numbers (x = 8, 7, 6, 5). Trifolium subterraneum and Trifolium israeliticum are rel...

journal_title：Genome

authors： Falistocco E,Marconi G,Falcinelli M

更新日期：2013-06-01 00:00:00

abstract：:The SRY gene is a single-copy, male-specific gene, located on the Y chromosome in most mammals. However, recently we have described the presence of multiple polymorphic copies of this gene in both males and females of the vole species Microtus cabrerae. Here, we present the chromosomal localization of SRY gene copies ...

journal_title：Genome

authors： Fernández R,Barragán MJ,Bullejos M,Marchal JA,Martínez S,Díaz de la Guardia R,Sánchez A

更新日期：2002-06-01 00:00:00

abstract：:We have used a PCR and cloning strategy to identify Ty1-copia-like retrotransposons in tomato, Lycopersicon esculentum Mill. Using degenerate oligonucleotide primers corresponding to conserved domains of the Ty1-copia retrotransposon reverse transcriptase (RT), fragments of about 260 bp were obtained by PCR amplificat...

journal_title：Genome

authors： Rogers SA,Pauls KP

更新日期：2000-10-01 00:00:00

abstract：:The evolution of karyotypically stabilized short-lived (SL) and long-lived (LL) cytoraces in the laboratory have been established and validated through our previous lifespan studies. In the present investigation, we examined the possible reason(s) for the differential longevity among selected members of SL and LL cyto...

journal_title：Genome

authors： Ranjini MS,Hosamani R,Muralidhara,Ramachandra NB

更新日期：2011-10-01 00:00:00

abstract：:Mutations in High temperature requirement A2 (HtrA2), also designated PARK13, which lead to the loss of its protease activity, have been associated with Parkinson's disease (PD). HtrA2 is a mitochondrial protease that translocates to the cytosol upon the initiation of apoptosis where it participates in the abrogation ...

journal_title：Genome

authors： M'Angale PG,Staveley BE

更新日期：2017-01-01 00:00:00

abstract：:During 30 years of unprecedented urbanization, plant diversity in Shenzhen, a young megacity in southern China, has increased dramatically. Although strongly associated with plant diversity, butterfly diversity generally declines with urbanization, but this has not been investigated in Shenzhen. Considering the speed ...

journal_title：Genome

authors： Sing KW,Dong H,Wang WZ,Wilson JJ

更新日期：2016-09-01 00:00:00

abstract：:The 5S rRNA genes from 2 species of the Ostreidae family, Crassostrea angulata and Crassostrea gigas, were molecularly characterized. The genes were amplified, cloned, and sequenced. The results revealed a 5S rDNA tandem array with a nucleotide sequence in an inverted position within the nontranscribed spacer region t...

journal_title：Genome

authors： Cross I,Rebordinos L

更新日期：2005-12-01 00:00:00

abstract：:Genomics is both data and compute intensive discipline. The success of genomics depends on adequate informatics infrastructure that can address growing data demands and enable a diverse range of resource-intensive computational activities. Designing a suitable Infrastructure is a challenging task, and its success larg...

journal_title：Genome

authors： Krishna R,Elisseev V

更新日期：2020-11-20 00:00:00

abstract：:Orchidaceae, one of the largest families of angiosperms, is represented in India by 1600 species distributed in diverse habitats. Orchids are in high demand owing to their beautiful flowers and therapeutic properties. Overexploitation and habitat destruction have made many orchid species endangered. In the absence of ...

journal_title：Genome

authors： Parveen I,Singh HK,Malik S,Raghuvanshi S,Babbar SB

更新日期：2017-08-01 00:00:00

abstract：:Eighteen low-copy and genomic microsatellite markers were tested for Mendelian inheritance and then assayed in 41 Pinus taeda L. samples drawn from five regions in the southern United States. The PCR products had multiple alleles, high levels of polymorphism, and little non-specific priming. Fifteen of the 18 markers ...

journal_title：Genome

authors： Elsik CG,Minihan VT,Hall SE,Scarpa AM,Williams CG

更新日期：2000-06-01 00:00:00

abstract：:Sequence-based markers have added a new dimension in the efficiency of identifying alien introgressions in wheat. Expressed sequence tag-sequence tagged sites (EST-STS) markers have proved useful in tracing alien chromatin. In this study, we report the development of Thinopyrum bessarabicum- and Secale anatolicum-spec...

journal_title：Genome

authors： Singh AK,Lo K,Dong C,Zhang P,Trethowan RM,Sharp PJ

更新日期：2020-11-01 00:00:00

abstract：:The use of consensus chloroplast microsatellites primers for dicotyledonous chloroplast genomes revealed the existence of intra and interspecific length variation within the genus Vitis. Three chloroplast microsatellite loci were found to be polymorphic in samples of Vitis vinifera, Vitis berlandieri, Vitis riparia, a...

journal_title：Genome

authors： Arroyo-García R,Lefort F,de Andrés MT,Ibáñaez J,Borrego J,Jouve N,Cabello F,Martínez-Zapater JM

更新日期：2002-12-01 00:00:00

abstract：:The establishment of a C-banded karyotype of hexaploid oat (Avena spp., 2n = 6x = 42) has facilitated the cytological characterization of a monosomic series in 'Kanota', an A. byzantina (C. Koch) cultivar. The 'Kanota' series of monosomics analyzed in this study consists of only 12 of the 21 different chromosome-defic...

journal_title：Genome

authors： Jellen EN,Rooney WL,Phillips RL,Rines HW

更新日期：1993-10-01 00:00:00

abstract：:Larvae of the black fly morphospecies Simulium vittatum from Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with 33P, and run on polyacrylamide gels. The entire da...

journal_title：Genome

authors： Duncan GA,Adler PH,Pruess KP,Powers TO

更新日期：2004-04-01 00:00:00

abstract：:Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis of these fragments uncovered eight unique classes of disease-resistance related sequenc...

journal_title：Genome

authors： Rivkin MI,Vallejos CE,McClean PE

更新日期：1999-02-01 00:00:00

abstract：:The aplocheiloid killifish Rivulus marmoratus is the only known self-fertilizing hermaphroditic vertebrate. Most natural populations consist almost entirely of hermaphrodites and comprise arrays of homozygous clones. However, in almost all populations thus far studied, clonal variation, as detected with molecular tech...

journal_title：Genome

authors： Sola L,Marzovillo M,Rossi AR,Gornung E,Bressanello S,Turner BJ

更新日期：1997-12-01 00:00:00

abstract：:Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...

journal_title：Genome

authors： Brooks SA,Huang L,Gill BS,Fellers JP

更新日期：2002-10-01 00:00:00

abstract：:Insulin-like growth factor 1 (IGF1) regulates growth and metabolic functions in vertebrates. A dinucleotide repeat sequence located at the promoter region of the IGF1 gene has been reported in several vertebrate species and may affect the control of the transcriptional activity of this gene. The genotypes of animals f...

journal_title：Genome

authors： Caetano AR,Bowling AT

更新日期：1998-02-01 00:00:00

abstract：:The purposes of the present research are (i) to establish the karyotype of Neursopora crassa using visualization of kinetochores in the synaptonemal complex (SC) spreads, (ii) to assign each chromosome to a linkage group, and (iii) to examine chromosome pairing and recombination nodules in quadrivalents. Two strains c...

journal_title：Genome

authors： Lu BC

更新日期：2006-06-01 00:00:00

abstract：:Ribosomal intergenic spacers (IGSs) of Odontophrynus americanus 2n and 4n were cloned, restriction mapped, and partially sequenced. Three distinct regions, namely alpha, beta, and delta, were identified in the IGSs. The alpha and beta regions flanked the 28S and 18S rRNA genes, respectively, conserving an identical re...

journal_title：Genome

authors： Alvares LE,Polanco C,Brison O,Coutinho LL,Ruiz IR

更新日期：2002-02-01 00:00:00

abstract：:Carrot (Daucus carota L.) chromosomes are small and poorly differentiated in size and morphology. Here we demonstrate that fluorescent in situ hybridization (FISH) signals derived from arbitrary PCR probes can be used for chromosome identification in carrot. To prepare probes, we searched for nonpolymorphic products a...

journal_title：Genome

authors： Nowicka A,Grzebelus E,Grzebelus D

更新日期：2012-03-01 00:00:00

abstract：:Multiple reaction monitoring mass spectrometry (MRM-MS) is an emerging technology for blood biomarker verification and validation; however, the results may be influenced by pre-analytical factors. This exploratory study was designed to determine if differences in phlebotomy techniques would significantly affect the ab...

journal_title：Genome

authors： Penn AM,Lu L,Chambers AG,Balshaw RF,Morrison JL,Votova K,Wood E,Smith DS,Lesperance M,del Zoppo GJ,Borchers CH,SpecTRA Study Group.

更新日期：2015-12-01 00:00:00

abstract：:Variation in recombinant frequencies at two adjacent intervals on chromosome 6 of tomato (Lycopersicon esculentum Mill.) has been studied in seven lines that differ in the amount and origin of introgressed segments from wild species. These lines were all crossed to a genotype homozygous recessive for the markers tl, y...

journal_title：Genome

authors： Liharska T,Wordragen M,Kammen A,Zabel P,Koornneef M

更新日期：1996-06-01 00:00:00

abstract：:A mapping population of Paspalum simplex segregating for apomixis (asexual reproduction through seeds) was screened with AFLPs to find apomixis-linked markers. Four AFLPs linked to apomixis in coupling phase were found. Three of them did not show recombinants among the 87 individuals of the mapping population, whereas...

journal_title：Genome

authors： Labombarda P,Busti A,Caceres ME,Pupilli F,Arcioni S

更新日期：2002-06-01 00:00:00

abstract：:Unisexual Ambystoma are the oldest known unisexual vertebrates and comprise a lineage of eastern North American all female salamanders that reproduce by stealing sperm from as many as five normally bisexual congeneric species. The sperm may be used to only stimulate egg development by gynogenesis but can be incorporat...

journal_title：Genome

authors： Bogart JP

更新日期：2019-08-01 00:00:00

abstract：:The "rings" belonging to the genus Ypthima are amongst the most common butterflies in Peninsular Malaysia. However, the species can be difficult to tell apart, with keys relying on minor and often non-discrete ring characters found on the hindwing. Seven species have been reported from Peninsular Malaysia, but this is...

journal_title：Genome

authors： Jisming-See SW,Sing KW,Wilson JJ

更新日期：2016-10-01 00:00:00

abstract：:The use of interspecific crosses in breeding is an important strategy in improving the genetic base of the modern cultivated potato, Solanum tuberosum L. Until now, it has normally been interspecific Solanum hybrids that have been morphologically and cytologically characterized. However, little is known about the geno...

journal_title：Genome

authors： Marfil CF,Masuelli RW,Davison J,Comai L

更新日期：2006-02-01 00:00:00