Identification and characterization of the Oct4 extended transcriptional regulatory region in Guanzhong dairy goat.

Abstract:

:The octamer-binding transcription factor 4 gene (Oct4) plays a critical role in maintaining pluripotency during early mammalian embryonic development and self-renewal of embryonic stem (ES) cells. In this study, we cloned the Oct4 cDNA and 2.8-kb regulatory region upstream of the start codon in Guanzhong dairy goat ( Capra hircus ). The comparative sequence analysis of Oct4 cDNA showed that it was highly conserved among six mammalian species. The goat Oct4 5' regulatory regions were homologous to the corresponding regions of Oct4 in other species and were functional in directing the expression of luciferase in mouse P19 embryonic carcinoma cells and mouse J1 ES cells. Furthermore, the methylation levels in the goat Oct4 minimal promoter and proximal enhancer in the fetal genital ridge were lower than those in the heart. Additionally, two processed pseudogenes that shared high homology with goat Oct4 cDNA were identified and characterized.

journal_name

Genome

journal_title

Genome

authors

Cheng X,Meng S,Deng J,Lai W,Wang H

doi

10.1139/g11-047

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

812-8

issue

10

eissn

0831-2796

issn

1480-3321

journal_volume

54

pub_type

杂志文章

相关文献

GENOME文献大全
  • A bacterial artificial chromosome library for 'Jefferson' hazelnut and identification of clones associated with eastern filbert blight resistance and pollen-stigma incompatibility.

    abstract::European hazelnut (Corylus avellana L.) is the only economically important nut crop in the family Betulaceae. Because of its small genome size (~385 Mb / 1C), relatively short life cycle, availability of a dense linkage map, and amenability to transformation by Agrobacterium, the European hazelnut could serve as a mod...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g11-048

    authors: Sathuvalli VR,Mehlenbacher SA

    更新日期:2011-10-01 00:00:00

  • Molecular characterization and chromosomal distribution of species-specific repetitive DNA sequences from Beta corolliflora, a wild relative of sugar beet.

    abstract::Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridizati...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g00-084

    authors: Gao D,Schmidt T,Jung C

    更新日期:2000-12-01 00:00:00

  • Can butterflies cope with city life? Butterfly diversity in a young megacity in southern China.

    abstract::During 30 years of unprecedented urbanization, plant diversity in Shenzhen, a young megacity in southern China, has increased dramatically. Although strongly associated with plant diversity, butterfly diversity generally declines with urbanization, but this has not been investigated in Shenzhen. Considering the speed ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0192

    authors: Sing KW,Dong H,Wang WZ,Wilson JJ

    更新日期:2016-09-01 00:00:00

  • An RFLP species-specific DNA sequence for the A genome of rice.

    abstract::A DNA sequence, pOs6.20, was cloned from the nuclear genome of cultivated rice (Oryza sativa L.) by hybridization with a human minisatellite sequence. At high hybridization stringency, a subfragment of the rice sequence, pOs6.20.3, detected low-copy restriction fragment length polymorphisms (RFLPs), which behaved as M...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-061

    authors: Dallas JF,McIntyre CL,Gustafson JP

    更新日期:1993-06-01 00:00:00

  • Gene expression patterns indicate that a high-fat-high-carbohydrate diet causes mitochondrial dysfunction in fish.

    abstract::Expensive and unsustainable fishmeal is increasingly being replaced with cheaper lipids and carbohydrates as sources of energy in aquaculture. Although it is known that the excess of lipids and carbohydrates has negative effects on nutrient utilization, growth, metabolic homeostasis, and health of fish, our current un...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0159

    authors: Prisingkorn W,Jakovlić I,Yi SK,Deng FY,Zhao YH,Wang WM

    更新日期:2019-02-01 00:00:00

  • Isolation and preliminary characterization of histone H1.b allelic variants from quail erythrocytes.

    abstract::Our goal was to purify and characterize the allelic variants H1b1 and H1b2 of histone H1.b, one of the seven subtypes of this linker histone extracted from Japanese quail erythrocyte nuclei. These variants are revealed phenotypically as band H1.3 or part of band H1.4 by polyacrylamide gel electrophoresis (PAGE) in sod...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Pałyga J,Neelin JM

    更新日期:1998-10-01 00:00:00

  • Disease-resistance related sequences in common bean.

    abstract::Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis of these fragments uncovered eight unique classes of disease-resistance related sequenc...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Rivkin MI,Vallejos CE,McClean PE

    更新日期:1999-02-01 00:00:00

  • Inheritance and expression patterns of BN28, a low temperature induced gene in Brassica napus, throughout the Brassicaceae.

    abstract::Molecular genetics is becoming an important tool in the breeding and selection of agronomically important traits. BN28 is a low temperature induced gene in Brassicaceae species. PCR and Southern blot analysis indicate that BN28 is polymorphic in the three diploid genomes: Brassica rapa (AA), Brassica nigra (BB), and B...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-089

    authors: Hawkins GP,Nykiforuk CL,Johnson-Flanagan AM,Boothe JG

    更新日期:1996-08-01 00:00:00

  • Insect mitochondrial genomics 2: The complete mitochondrial genome sequence of a giant stonefly, Pteronarcys princeps, asymmetric directional mutation bias, and conserved plecopteran A+T-region elements.

    abstract::Mitochondrial (mt) genome sequences of insects are receiving renewed attention in molecular phylogentic studies, studies of mt-genome rearrangement, and other unusual molecular phenomena, such as translational frameshifting. At present, the basal neopteran lineages are poorly represented by mt-genome sequences. Comple...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-037

    authors: Stewart JB,Beckenbach AT

    更新日期:2006-07-01 00:00:00

  • Mitotic and polytene chromosome analyses in the Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae).

    abstract::The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capit...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Zhao JT,Frommer M,Sved JA,Zacharopoulou A

    更新日期:1998-08-01 00:00:00

  • Genetic and cytological mapping of a "Y-2" translocation in the Mediterranean fruit fly Ceratitis capitata.

    abstract::In this paper we analyze genetically and cytologically a Y-chromosome 2 translocation with several markers, some of which are potentially useful for large scale sex separation. The breakpoint of this Y-2 translocation is located at region 6B on the trichogene polytene chromosome map. It is found that, in strains carry...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-145

    authors: Cladera JL,Delprat MA

    更新日期:1995-12-01 00:00:00

  • Intraspecific sequence variation in the mitochondrial genome of rainbow trout (Oncorhynchus mykiss).

    abstract::We examined the sequence of a 2214 base pair HindIII fragment from the mitochondrial genome of six rainbow trout. The fragment encodes four proteins and two tRNAs. Sequences for two fish from a single locality were identical. Those from separate localities differed by from 1 to 7 nucleotide substitutions. Of 13 variab...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g90-003

    authors: Beckenbach AT,Thomas WK,Sohrabi H

    更新日期:1990-02-01 00:00:00

  • Multiple flowering time QTLs within several Brassica species could be the result of duplicated copies of one ancestral gene.

    abstract::Quantitative trait locus (QTL) analysis was used to study the evolution of genes controlling the timing of flowering in four Brassica genomes that are all extensively replicated. Comparative mapping showed that a chromosomal region from the top of Arabidopsis thaliana chromosome 5 corresponded to three homoeologous co...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Axeisson T,Shavorskaya O,Lagercrantz U

    更新日期:2001-10-01 00:00:00

  • Localization of the 68,000-Da human neurofilament gene (NF68) using a murine cDNA probe.

    abstract::A recent investigation, using a human genomic probe, has indicated that the 68,000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p less than 0.0001). In addition, we have fou...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g88-083

    authors: Somerville MJ,McLachlan DR,Percy ME

    更新日期:1988-08-01 00:00:00

  • Full-length transcriptome analysis and identification of genes involved in asarinin and aristolochic acid biosynthesis in medicinal plant Asarum sieboldii.

    abstract::Asarum sieboldii a well-known traditional Chinese medicinal herb, is used for curing inflammation and ache. It contains both bioactive ingredient asarinin and the toxic compound aristolochic acid. To address further breeding demand, genes involved in the biosynthetic pathways of asarinin and aristolochic acid should b...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0095

    authors: Chen C,Shi X,Zhou T,Li W,Li S,Bai G

    更新日期:2020-12-15 00:00:00

  • Segmental chromosomal duplications harbouring group IV CONSTANS-like genes in cereals.

    abstract::Comparative mapping is an important component of map-based cloning in large-genome cereal species. We describe evidence of a segmental chromosomal duplication harbouring CONSTANS-like genes in barley that predates the divergence of the Oryzoideae (rice) and Pooideae (brachypodium, barley, wheat) clades, and discuss th...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-101

    authors: Cockram J,Howells RM,O'Sullivan DM

    更新日期:2010-03-01 00:00:00

  • The use of RAPD for identifying and classifying Musa germplasm.

    abstract::Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pattern of variation o...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-045

    authors: Howell EC,Newbury HJ,Swennen RL,Withers LA,Ford-Lloyd BV

    更新日期:1994-04-01 00:00:00

  • Microsatellite marker analysis of an anther-derived potato family: skewed segregation and gene-centromere mapping.

    abstract::Segregation patterns of polymorphic simple sequence repeat (SSR) primer pairs were investigated in monoploid potato families derived from anther culture. A total of 14 primers developed from the sequences in the database, as well as from a genomic library of potato, was used. Distorted segregation was observed for sev...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g01-140

    authors: Chani E,Ashkenazi V,Hillel J,Veilleux RE

    更新日期:2002-04-01 00:00:00

  • Colocalization of antisense RNAs and ribozymes with their target mRNAs.

    abstract::The use of complementary RNA sequences such as antisense RNAs and ribozymes to regulate the expression of specific genes in eukaryotic cells has been well-documented, particularly with their application to both human gene therapy and plant biotechnology. Despite the simplicity of this approach, this technique usually ...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/g97-802

    authors: Arndt GM,Rank GH

    更新日期:1997-12-01 00:00:00

  • Characterization and mapping of a male-sterility mutant, tapetum desquamation (t), in rice.

    abstract::A spontaneously mutated male-sterile material was found among the offspring of the indica restorer line Jinhuiyihao. To understand the status and function of the related gene and clone the gene, a near-isogenic line (NIL) of the male sterility was bred, and characterization of the mutant and gene mapping were performe...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-013

    authors: Zhang Y,Mao JX,Yang K,Li YF,Zhang J,Huang YX,Shen FC,Zhang CD

    更新日期:2008-05-01 00:00:00

  • Molecular analysis of evolutionary patterns in U genome wild wheats.

    abstract::The theory of pivotal-differential evolution states that one genome of polyploid wheats remains stable (i.e., pivotal) during evolution, while the other genome or genomes may become modified (i.e., differential). A proposed mechanism for apparent modification of the differential genome is that different polyploid spec...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-036

    authors: Chee PW,Lavin M,Talbert LE

    更新日期:1995-04-01 00:00:00

  • Efficiency of RFLP, RAPD, and AFLP markers for the construction of an intraspecific map of the tomato genome.

    abstract::We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment le...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Saliba-Colombani V,Causse M,Gervais L,Philouze J

    更新日期:2000-02-01 00:00:00

  • New porcine microRNA genes found by homology search.

    abstract::MicroRNAs (miRNAs) repress target genes at the post-transcriptional level and play important roles in development and cell lineage decisions. In vertebrates, however, both the targets of miRNAs and their expression profiles during development are poorly understood. Thus far, 326 human miRNAs, 249 mouse miRNAs, and 195...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-120

    authors: Kim HJ,Cui XS,Kim EJ,Kim WJ,Kim NH

    更新日期:2006-10-01 00:00:00

  • Chromosome segregation from cell hybrids. III. Segregation is independent of spindle constitution.

    abstract::Hamster beta-tubulin (detected as a mutant subunit that confers Colcemid resistance) is either not expressed or is underexpressed in Chinese hamster-mouse somatic cell hybrids. This selectivity of tubulin expression suggests that a uniparental mouse spindle might preferentially engage mouse chromosomes and lead to los...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g87-090

    authors: Zelesco PA,Graves JA

    更新日期:1987-08-01 00:00:00

  • Association of morphological and RFLP markers in rice (Oryza sativa L.).

    abstract::Seventeen morphological marker genes were associated with restriction fragment length polymorphism markers in rice by using four F2 populations, each segregating for a few observable traits, and 14 near isogenic lines (NILs), each containing one morphological mutant gene. The location of five genes was confirmed on th...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-073

    authors: Yu ZH,McCouch SR,Tanksley SD,Kinoshita T,Sato S

    更新日期:1995-06-01 00:00:00

  • Transposition of minisatellite-like DNA in Chironomus midges.

    abstract::Cla elements are a family of tandem repetitive DNA sequences present in the genome of several Chironomus species. Interspersed clusters of Cla elements are widely distributed all over the chromosomes in C. thummi thummi, while they seem to be limited to the centromeric regions in the closely related subspecies C. t. p...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-077

    authors: Hankeln T,Rohwedder A,Weich B,Schmidt ER

    更新日期:1994-08-01 00:00:00

  • Analysis of genetic diversity in red clover (Trifolium pratense L.) breeding populations as revealed by RAPD genetic markers.

    abstract::Red clover is an important forage legume species for temperate regions and very little is known about the genetic organization of its breeding populations. We used random amplified polymorphic DNA (RAPD) genetic markers to address the genetic diversity and the distribution of variation in 20 breeding populations and c...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-030

    authors: Ulloa O,Ortega F,Campos H

    更新日期:2003-08-01 00:00:00

  • A family study to examine clonal diversity in unisexual salamanders (genus Ambystoma).

    abstract::Unisexual Ambystoma are the oldest known unisexual vertebrates and comprise a lineage of eastern North American all female salamanders that reproduce by stealing sperm from as many as five normally bisexual congeneric species. The sperm may be used to only stimulate egg development by gynogenesis but can be incorporat...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2019-0034

    authors: Bogart JP

    更新日期:2019-08-01 00:00:00

  • De novo balanced complex chromosome rearrangements involving chromosomes 1B and 3B of wheat and 1R of rye.

    abstract::Complex chromosome rearrangements (CCRs) are defined as structural abnormalities involving more than two chromosome breaks, coupled with exchanges of chromosomal segments. Information on CCRs in plants is limited. In the present study, a plant (26-4) harboring translocation chromosomes 1RS.1BL and 4RS.4DL was selected...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0112

    authors: Ren T,Li Z,Yan B,Tan F,Tang Z,Fu S,Yang M,Ren Z

    更新日期:2016-12-01 00:00:00

  • Isochromosome Y in an infertile heifer.

    abstract::The chromosome makeup of a 3-year-old crossbred heifer was examined in an attempt to explore the cytogenetic causes of her infertility. The heifer proved to be a mosaic carrying predominantly XY cells and with approximately 30% of cells lacking a normal Y. The minor population included cells carrying a dicentric isoch...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g90-104

    authors: Pinheiro LE,Mikich AB,Bechara GH,Almeida IL,Basrur PK

    更新日期:1990-10-01 00:00:00