A bacterial artificial chromosome library for 'Jefferson' hazelnut and identification of clones associated with eastern filbert blight resistance and pollen-stigma incompatibility.

Abstract:

:European hazelnut (Corylus avellana L.) is the only economically important nut crop in the family Betulaceae. Because of its small genome size (~385 Mb / 1C), relatively short life cycle, availability of a dense linkage map, and amenability to transformation by Agrobacterium, the European hazelnut could serve as a model plant for the Betulaceae. Here we report the construction of a bacterial artificial chromosome (BAC) library for 'Jefferson' hazelnut using the cloning enzyme MboI and the vector pECBAC1 (BamHI site). The library consists of 39,936 clones arrayed in 104,384-well microtitre plates with a mean insert size of 117 kb. The genomic coverage of the library is estimated to be about 12 genome equivalents. This library provides a valuable resource for the map-based cloning of two important genes, the resistance gene from 'Gasaway' that confers resistance to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Müller and the S locus that controls pollen-stigma incompatibility. Fine-resolution mapping near the two loci was carried out using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers. Fine mapping at the disease resistance locus showed that markers W07-375 and X01-825 flanked the resistance locus at distances of 0.06 and 0.05 cM, respectively. The S locus is flanked by markers 204-950 and KG819-200 at distances of 0.14 and 0.24 cM, respectively. Assuming that 1 cM corresponds to a physical distance of 430 kb, it will take approximately two to three chromosome walks to assemble BAC contigs that span both loci.

journal_name

Genome

journal_title

Genome

authors

Sathuvalli VR,Mehlenbacher SA

doi

10.1139/g11-048

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

862-7

issue

10

eissn

0831-2796

issn

1480-3321

journal_volume

54

pub_type

杂志文章

相关文献

GENOME文献大全
  • Assessment of genetic variation and differentiation of hop genotypes by microsatellite and AFLP markers.

    abstract::Microsatellites have many desirable marker properties and have been increasingly used in crop plants in genetic diversity studies. Here we report on the characterisation of microsatellite markers and on their use for the determination of genetic identities and the assessment of genetic variability among accessions fro...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-44-5-773

    authors: Jakse J,Kindlhofer K,Javornik B

    更新日期:2001-10-01 00:00:00

  • SNP discovery by amplicon sequencing and multiplex SNP genotyping in the allopolyploid species Brassica napus.

    abstract::Oilseed rape (Brassica napus) is an allotetraploid species consisting of two genomes, derived from B. rapa (A genome) and B. oleracea (C genome). The presence of these two genomes makes single nucleotide polymorphism (SNP) marker identification and SNP analysis more challenging than in diploid species, as for a given ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G10-079

    authors: Durstewitz G,Polley A,Plieske J,Luerssen H,Graner EM,Wieseke R,Ganal MW

    更新日期:2010-11-01 00:00:00

  • Genomic in situ hybridization (GISH) reveals high chromosome pairing affinity between Lolium perenne and Festuca mairei.

    abstract::Intergeneric hybridizations have been made between species of Lolium and Festuca. It has been demonstrated, largely through conventional cytogenetic analysis, that the genomes of the two genera are related, however, much information is lacking on exactly how closely related the genomes are between the two species. We ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Cao M,Sleper DA,Dong F,Jiang J

    更新日期:2000-04-01 00:00:00

  • Identification of plant genes regulated in resistant potato Solanum sparsipilum during the early stages of infection by Globodera pallida.

    abstract::Using a complementary (c)DNA-amplified fragment length polymorphism (AFLP) approach, we investigated differential gene expression linked to resistance mechanisms during the incompatible potato - Globodera pallida interaction. Expression was compared between a resistant and a susceptible potato clone, inoculated or not...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g07-015

    authors: Jolivet K,Grenier E,Bouchet JP,Esquibet M,Kerlan MC,Caromel B,Mugniéry D,Lefebvre V

    更新日期:2007-04-01 00:00:00

  • Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population.

    abstract::Fusarium head blight (FHB) is one of the most important fungal wheat diseases worldwide. Understanding the genetics of FHB resistance is the key to facilitating the introgression of different FHB resistance genes into adapted wheat. The objectives of the present study were to detect and map quantitative trait loci (QT...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-104

    authors: Yang Z,Gilbert J,Fedak G,Somers DJ

    更新日期:2005-04-01 00:00:00

  • Haplotype characterization and markers at the barley Mlo powdery mildew resistance locus as tools for marker-assisted selection.

    abstract::Recessive mlo alleles of the barley Mlo gene confer resistance to almost all known isolates of the powdery mildew fungal pathogen targeting barley (Hordeum vulgare). To characterize haplotypes present in the Mlo chromosomal region of cultivated Mlo and mlo barley genotypes, we conducted a polymorphism search in 3 pred...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-044

    authors: Tacconi G,Baldassarre V,Collins NC,Bulgarelli D,Stanca AM,Valè G

    更新日期:2006-08-01 00:00:00

  • Application of resistance gene analog markers to analyses of genetic structure and diversity in rice.

    abstract::Plant disease resistance gene analog (RGA) markers were designed according to the conserved sequence of known RGAs and used to map resistance genes. We used genome-wide RGA markers for genetic analyses of structure and diversity in a global rice germplasm collection. Of the 472 RGA markers, 138 were polymorphic and th...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2012-0142

    authors: Ren J,Yu Y,Gao F,Zeng L,Lu X,Wu X,Yan W,Ren G

    更新日期:2013-07-01 00:00:00

  • Gene flow, recombination, and positive selection in Stenotrophomonas maltophilia: mechanisms underlying the diversity of the widespread opportunistic pathogen.

    abstract::Stenotrophomonas maltophilia is a global multidrug-resistant human opportunistic pathogen in clinical environments. Stenotrophomonas maltophilia is also ubiquitous in aqueous environments, soil, and plants. Various molecular typing methods have revealed that S. maltophilia exhibits high levels of phenotypic and genoty...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0073

    authors: Yu D,Yin Z,Li B,Jin Y,Ren H,Zhou J,Zhou W,Liang L,Yue J

    更新日期:2016-12-01 00:00:00

  • Analysis of genetic diversity in red clover (Trifolium pratense L.) breeding populations as revealed by RAPD genetic markers.

    abstract::Red clover is an important forage legume species for temperate regions and very little is known about the genetic organization of its breeding populations. We used random amplified polymorphic DNA (RAPD) genetic markers to address the genetic diversity and the distribution of variation in 20 breeding populations and c...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-030

    authors: Ulloa O,Ortega F,Campos H

    更新日期:2003-08-01 00:00:00

  • A physical map of the Thinopyrum-derived Lr19 translocation.

    abstract::Twenty-nine lines with deletions in the Lr19 ('Indis') translocated chromosome segment were used to physically map three Thinopyrum RFLP loci as well as the Sr25 and Sd1 loci. From the data, the relative locations of marker loci on the translocation were determined as: Sd1, Xpsr165, Xpsr105, Xps129, Lr19, Wsp-D1, Sr25...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-126

    authors: Prins R,Marais GF,Marais AS,Janse BJ,Pretorius ZA

    更新日期:1996-10-01 00:00:00

  • Identification of a new class of recombinant prolamin genes in wheat.

    abstract::A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshif...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-042

    authors: Nagy IJ,Takács I,Juhász A,Tamás L,Bedo Z

    更新日期:2005-10-01 00:00:00

  • Molecular characterization of a tandem repeat, Afa family, and its distribution among Triticeae.

    abstract::We have characterized a so-called D genome specific repetitive DNA sequence (pAs1) of Aegilops squarrosa L. (2n = 14, genome DD) with respect to its DNA sequence and its distribution among Triticeae species. The clone consisted of three units of a repetitive DNA sequence of 336 or 337 base pairs, and was AT rich (65.2...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-063

    authors: Nagaki K,Tsujimoto H,Isono K,Sasakuma T

    更新日期:1995-06-01 00:00:00

  • Analysis of codon usage patterns in Morus notabilis based on genome and transcriptome data.

    abstract::Codons play important roles in regulating gene expression levels and mRNA half-lives. However, codon usage and related studies in multicellular organisms still lag far behind those in unicellular organisms. In this study, we describe for the first time genome-wide patterns of codon bias in Morus notabilis (mulberry tr...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0129

    authors: Wen Y,Zou Z,Li H,Xiang Z,He N

    更新日期:2017-06-01 00:00:00

  • Quantitative trait locus analysis of multiple agronomic traits in the model legume Lotus japonicus.

    abstract::The first quantitative trait locus (QTL) analysis of multiple agronomic traits in the model legume Lotus japonicus was performed with a population of recombinant inbred lines derived from Miyakojima MG-20 x Gifu B-129. Thirteen agronomic traits were evaluated in 2004 and 2005: traits of vegetative parts (plant height,...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g07-040

    authors: Gondo T,Sato S,Okumura K,Tabata S,Akashi R,Isobe S

    更新日期:2007-07-01 00:00:00

  • Mapping of quantitative trait loci for fire blight resistance in the apple cultivars 'Florina' and 'Nova Easygro'.

    abstract::Fire blight is a devastating bacterial disease of rosaceous plants. Its damage to apple production is a major concern, since no existing control option has proven to be completely effective. Some commercial apple varieties, such as 'Florina' and 'Nova Easygro', exhibit a consistent level of resistance to fire blight. ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g10-047

    authors: Le Roux PM,Khan MA,Broggini GA,Duffy B,Gessler C,Patocchi A

    更新日期:2010-09-01 00:00:00

  • A comparative study of the structure of the rDNA intergenic spacer of Lens culinaris Medik., and other legume species.

    abstract::As part of a project on lentil molecular genetics, the sequence of the 18S-25S ribosomal RNA gene intergenic spacer (IGS) of Lens culinaris Medik. was determined. DNA was cloned after polymerase chain reaction (PCR) amplification. The spacer of 2939 bp was composed of nonrepetitive sequences and four tandem arrays of ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g00-022

    authors: Fernández M,Polanco C,Ruiz ML,Pérez de la Vega M

    更新日期:2000-08-01 00:00:00

  • 5S rDNA genome regions of Lens species.

    abstract::The length variability of the nontranscribed spacer (NTS) of the 5S rDNA repeats was analyzed in species of the genus Lens by means of PCR amplification. The NTS ranged from approximately 227 to approximately 952 bp. The polymorphism detected was higher than previous NTS polymorphisms described in this genus. Three NT...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-052

    authors: Fernández M,Ruiz ML,Linares C,Fominaya A,Pérez de la Vega M

    更新日期:2005-10-01 00:00:00

  • Gametocidal genes of Aegilops: segregation distorters in wheat-Aegilops wide hybridization.

    abstract::Aegilops is a genus belonging to the family Poaceace, which have played an indispensible role in the evolution of bread wheat and continues to do so by transferring genes by wide hybridization. Being the secondary gene pool of wheat, gene transfer from Aegilops poses difficulties and segregation distortion is common. ...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/gen-2017-0023

    authors: Niranjana M

    更新日期:2017-08-01 00:00:00

  • Standard karyotype of Triticum longissimum and its cytogenetic relationship with T. aestivum.

    abstract::C-banding polymorphism was analyzed in 17 accessions of Triticum longissimum from Israel and Jordan, and a generalized idiogram of this species was established. C-banding analysis was further used to identify two sets of disomic T. aestivum - T. longissimum chromosome addition lines and 13 ditelosomic addition lines a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-098

    authors: Friebe B,Tuleen N,Jiang J,Gill BS

    更新日期:1993-08-01 00:00:00

  • Identification of ISSR markers associated with productivity traits in silkworm, Bombyx moni L.

    abstract::Bombyx mori L., commonly recognised around the world as the mulberry silkworm, is characterized by a wide variability in yield and developmental traits, which have been proven through conventional genetic analysis to be of polygenic nature. A large number of morpho-biochemical traits and RFLP and RAPD markers are mapp...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-024

    authors: Chatterjee SN,Mohandas TP

    更新日期:2003-06-01 00:00:00

  • Evolutionary distribution of light-harvesting complex-like proteins in photosynthetic eukaryotes.

    abstract::Light-harvesting-like (LIL) proteins are low-molecular-mass membrane proteins related to the light-harvesting complexes, which form the dominant antenna system in most photosynthetic eukaryotes. To analyze the LIL protein family, we mined a number of publicly available databases to identify members of this family in a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g09-081

    authors: Neilson JA,Durnford DG

    更新日期:2010-01-01 00:00:00

  • A minisatellite polymorphism in intron III of the barramundi (Lates calcarifer) growth hormone gene.

    abstract::This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-117

    authors: Yowe DL,Epping RJ

    更新日期:1996-10-01 00:00:00

  • DNA barcoding reveals CITES-listed species among Taiwanese government-seized chelonian specimens.

    abstract::Compared to traditional morphological identification, DNA barcoding-molecular identification based on sequencing of a segment of mitochondrial cytochrome c oxidase subunit I (COI)-provides a shortcut to authenticating chelonian identifications. Here, we selected 63 government-seized chelonian specimens deposited at Ta...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2017-0264

    authors: Chang CH,Dai WY,Chen TY,Lee AH,Hou HY,Liu SH,Jang-Liaw NH

    更新日期:2018-08-01 00:00:00

  • Centromeric heterochromatin and satellite DNA in the Chironomus plumosus species group.

    abstract::Species of the Chironomus plumosus group display significant differences in their amount of centromeric heterochromatin. A tandem-repetitive satellite-like DNA has been isolated from C. plumosus. This DNA accounts for a major part of the centromeric heterochromatin. The DNA element has a Sau3AI restriction site ("Sau ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-132

    authors: Hankeln T,Fillippova MA,Kiknadze II,Aimanova KG,Schmidt ER

    更新日期:1994-12-01 00:00:00

  • FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor.

    abstract::Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-063

    authors: Gómez MI,Islam-Faridi MN,Woo SS,Czeschin D Jr,Zwick MS,Stelly DM,Price HJ,Schertz KF,Wing RA

    更新日期:1997-08-01 00:00:00

  • QTL mapping of seed-quality traits in sunflower recombinant inbred lines under different water regimes.

    abstract::The objectives of the present research were to determine the effects of water stress on seed-quality traits and to map QTLs controlling the studied traits under two different water treatments in a population of sunflower recombinant inbred lines (RILs). Two experiments were conducted in greenhouse and field conditions...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-038

    authors: Ebrahimi A,Maury P,Berger M,Kiani SP,Nabipour A,Shariati F,Grieu P,Sarrafi A

    更新日期:2008-08-01 00:00:00

  • Quantitative trait loci on barley (Hordeum vulgare L.) chromosome 7 associated with components of winterhardiness.

    abstract::Quantitative trait loci (QTL) controlling traits associated with winterhardiness in barley (field survival, LT50, growth habit, and crown fructan content) were mapped to chromosome 7 in a population of 100 F1-derived doubled haploid lines. The largest QTL effects for all traits were detected in a 21% recombination int...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-009

    authors: Hayes PM,Blake T,Chen TH,Tragoonrung S,Chen F,Pan A,Liu B

    更新日期:1993-02-01 00:00:00

  • Disease-resistance related sequences in common bean.

    abstract::Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis of these fragments uncovered eight unique classes of disease-resistance related sequenc...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Rivkin MI,Vallejos CE,McClean PE

    更新日期:1999-02-01 00:00:00

  • Multiple origins of cultivated radishes as evidenced by a comparison of the structural variations in mitochondrial DNA of Raphanus.

    abstract::Configurations of mitochondrial coxI and orfB gene regions were analysed by polymerase chain reaction (PCR) in three wild and one cultivated species of Raphanus. A total of 207 individual plants from 60 accessions were used. PCR with five combinations of primers identified five different amplification patterns both in...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-110

    authors: Yamagishi H,Terachi T

    更新日期:2003-02-01 00:00:00

  • A single molecular marker to distinguish between species of Dioscorea.

    abstract::Yams are species of the genus Dioscorea (family Dioscoreaceae), which consists of approximately 630 species. The majority of the world production of yams occurs in Africa with 58.8 million t annually, but they are also produced in the Americas and Asia. The saponins in yams have been reported to possess various proper...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0105

    authors: Techen N,Parveen I,Khan IA

    更新日期:2017-03-01 00:00:00