Abstract:
:A DNA sequence, pOs6.20, was cloned from the nuclear genome of cultivated rice (Oryza sativa L.) by hybridization with a human minisatellite sequence. At high hybridization stringency, a subfragment of the rice sequence, pOs6.20.3, detected low-copy restriction fragment length polymorphisms (RFLPs), which behaved as Mendelian genetic markers. This subfragment detected multicopy RFLPs between an indica and a javanica cultivar at medium hybridization stringency. The sequences detected by pOs6.20.3 at high hybridization stringency did not occur in rice species possessing the B, C, D, E, and F genomes. These results suggest that the RFLPs detected by this sequence can be placed on a genetic map of rice and that this sequence can be used as a species-specific probe for the A genome of rice.
journal_name
Genomejournal_title
Genomeauthors
Dallas JF,McIntyre CL,Gustafson JPdoi
10.1139/g93-061subject
Has Abstractpub_date
1993-06-01 00:00:00pages
445-8issue
3eissn
0831-2796issn
1480-3321pii
g93-061journal_volume
36pub_type
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