An RFLP species-specific DNA sequence for the A genome of rice.

Abstract:

:A DNA sequence, pOs6.20, was cloned from the nuclear genome of cultivated rice (Oryza sativa L.) by hybridization with a human minisatellite sequence. At high hybridization stringency, a subfragment of the rice sequence, pOs6.20.3, detected low-copy restriction fragment length polymorphisms (RFLPs), which behaved as Mendelian genetic markers. This subfragment detected multicopy RFLPs between an indica and a javanica cultivar at medium hybridization stringency. The sequences detected by pOs6.20.3 at high hybridization stringency did not occur in rice species possessing the B, C, D, E, and F genomes. These results suggest that the RFLPs detected by this sequence can be placed on a genetic map of rice and that this sequence can be used as a species-specific probe for the A genome of rice.

journal_name

Genome

journal_title

Genome

authors

Dallas JF,McIntyre CL,Gustafson JP

doi

10.1139/g93-061

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

445-8

issue

3

eissn

0831-2796

issn

1480-3321

pii

g93-061

journal_volume

36

pub_type

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