Full-length transcriptome analysis and identification of genes involved in asarinin and aristolochic acid biosynthesis in medicinal plant Asarum sieboldii.

Abstract:

:Asarum sieboldii a well-known traditional Chinese medicinal herb, is used for curing inflammation and ache. It contains both bioactive ingredient asarinin and the toxic compound aristolochic acid. To address further breeding demand, genes involved in the biosynthetic pathways of asarinin and aristolochic acid should be explored. Therefore, we sequenced the full-length transcriptome of A. sieboldii using PacBio Iso-Seq to determine the candidate transcripts that encode the biosynthetic enzymes of asarinin and aristolochic acid. In our study, in total of 63,023 full-length transcripts were generated with an average length of 1,371 bp from roots, stems and leaves tissues, of which 49,593 transcripts (78.69%) were annotated against public databases. Furthermore, 555 alternative splicing (AS), 10,869 lncRNAs as well as their 11,291 target genes and 17,909 SSRs were identified. Our data also revealed that 97 candidate transcripts related to asarinin metabolism, of which 6 novel genes that encoded enzymes involved in asarinin biosynthesis were initially reported. 56 transcripts related to aristolochic acid biosynthesis were also identified, especially CYP81B. In summary, our transcriptome data provide a useful resource to study gene function and genetic engineering in A. sieboldii.

journal_name

Genome

journal_title

Genome

authors

Chen C,Shi X,Zhou T,Li W,Li S,Bai G

doi

10.1139/gen-2020-0095

subject

Has Abstract

pub_date

2020-12-15 00:00:00

eissn

0831-2796

issn

1480-3321

pub_type

杂志文章

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