Abstract:
:Asarum sieboldii a well-known traditional Chinese medicinal herb, is used for curing inflammation and ache. It contains both bioactive ingredient asarinin and the toxic compound aristolochic acid. To address further breeding demand, genes involved in the biosynthetic pathways of asarinin and aristolochic acid should be explored. Therefore, we sequenced the full-length transcriptome of A. sieboldii using PacBio Iso-Seq to determine the candidate transcripts that encode the biosynthetic enzymes of asarinin and aristolochic acid. In our study, in total of 63,023 full-length transcripts were generated with an average length of 1,371 bp from roots, stems and leaves tissues, of which 49,593 transcripts (78.69%) were annotated against public databases. Furthermore, 555 alternative splicing (AS), 10,869 lncRNAs as well as their 11,291 target genes and 17,909 SSRs were identified. Our data also revealed that 97 candidate transcripts related to asarinin metabolism, of which 6 novel genes that encoded enzymes involved in asarinin biosynthesis were initially reported. 56 transcripts related to aristolochic acid biosynthesis were also identified, especially CYP81B. In summary, our transcriptome data provide a useful resource to study gene function and genetic engineering in A. sieboldii.
journal_name
Genomejournal_title
Genomeauthors
Chen C,Shi X,Zhou T,Li W,Li S,Bai Gdoi
10.1139/gen-2020-0095subject
Has Abstractpub_date
2020-12-15 00:00:00eissn
0831-2796issn
1480-3321pub_type
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