Detection of cytosine methylation and mapping of a gene influencing cytosine methylation in the genome of Citrus.

Abstract:

:A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1 hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification the DNA templates were digested with 2 pairs of restriction endonucleases (HpaII-MspI and (or) Sau3AI-NdeII) with different sensitivities to cytosine methylation and after PCR amplification their amplified products were further digested with the same enzymes. Using this method, it was possible to detect 28 methylation events involving 23 amplified bands with the 7 random primers and 2 pairs of enzymes. A methylation polymorphism was found at a Sau3AI site in a 1.2-kb band amplified with one primer. One locus influencing cytosine methylation at this restriction site was identified through genetic analysis of a BC1 population between C. grandis and P. trifoliata and was mapped to linkage group IV using an already developed core map. This technique for detecting methylation and methylation polymorphisms is simple and should be applicable to any eukaryotic species and to many situations where it is desirable to determine whether a sequence is methylated.

journal_name

Genome

journal_title

Genome

authors

Cai Q,Guy CL,Moore GA

doi

10.1139/g96-032

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

235-42

issue

2

eissn

0831-2796

issn

1480-3321

journal_volume

39

pub_type

杂志文章

相关文献

GENOME文献大全
  • Fluorescent in situ hybridization of a bacterial artificial chromosome.

    abstract::Fluorescent in situ hybridization (FISH) of a 130 kilobase cotton (Gossypium hirsuitum L.) bacterial artificial chromosome (BAC) clone containing a high proportion of single-copy DNA produced a large pair of FISH signals on the distal end of the long arm of a pair of chromosomes of the D-genome species G. raimondii Ul...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-082

    authors: Hanson RE,Zwick MS,Choi S,Islam-Faridi MN,McKnight TD,Wing RA,Price HJ,Stelly DM

    更新日期:1995-08-01 00:00:00

  • Three new satellite sequences and a mobile element found inside HSP70 introns of the Mediterranean mussel (Mytilus galloprovincialis).

    abstract::We report the characterization of 3 new repetitive sequences from the bivalve mollusc Mytilus galloprovincialis, designated Mg1, Mg2, and Mg3, with monomer lengths of 169, 260, and 70 bp, respectively. The 3 repeats together constitute approximately 7.8% of the M. galloprovincialis genome and were found, together with...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-111

    authors: Kourtidis A,Drosopoulou E,Pantzartzi CN,Chintiroglou CC,Scouras ZG

    更新日期:2006-11-01 00:00:00

  • Genome flux in tomato cell clones cultured in vitro in different physiological equilibria. II. A RAPD analysis of variability.

    abstract::An analysis of the effect of changing physiological conditions on genome evolution in tomato cell populations has been carried out on long-term in vitro cultured clones grown on different auxin-cytokinin equilibria or selected for low-high competence for active defense against Fusarium oxysporum f.sp. lycopersici. RAP...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-107

    authors: Bogani P,Simoni A,Lio' P,Scialpi A,Buiatti M

    更新日期:1996-10-01 00:00:00

  • Robustness of QTLs across germplasm pools using a model quantitative trait.

    abstract::Knowledge of the inheritance of C-glycosyl flavone synthesis in maize (Zea mays L.) silk tissues has been acquired through detailed genetic studies involving primarily germplasm from the Corn Belt Dent race. To test the robustness of this genetic knowledge, we examined C-glycosyl flavone synthesis in a genetically dis...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/G08-100

    authors: Lee EA,Staebler JM,Grainger C,Snook ME

    更新日期:2009-01-01 00:00:00

  • Phylogeny and expression of paralogous and orthologous sulphate transporter genes in diploid and hexaploid wheats.

    abstract::Twelve genes encoding two closely related subtypes (ST1.1a and ST1.1b) of a sulphate transporter have been identified in the diploid wheats Aegilops tauschii, Triticum urartu, and Aegilops speltoides, as well as the hexaploid Triticum aestivum. Based on phylogenetic comparisons with other plant sulphate transporters, ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-011

    authors: Buchner P,Prosser IM,Hawkesford MJ

    更新日期:2004-06-01 00:00:00

  • Mitotic and polytene chromosome analyses in the Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae).

    abstract::The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capit...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Zhao JT,Frommer M,Sved JA,Zacharopoulou A

    更新日期:1998-08-01 00:00:00

  • A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum x Gossypium barbadense backcross population.

    abstract::An interspecific Gossypium hirsutum x Gossypium barbadense backcross population of 75 BC1 plants was evaluated for 1014 markers. The map consists of 888 loci, including 465 AFLPs, 229 SSRs, 192 RFLPs, and 2 morphological markers, ordered in 37 linkage groups that represent most if not all of the 26 chromosomes, altoge...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-050

    authors: Lacape JM,Nguyen TB,Thibivilliers S,Bojinov B,Courtois B,Cantrell RG,Burr B,Hau B

    更新日期:2003-08-01 00:00:00

  • Chromosome banding patterns in the annual species of Cicer.

    abstract::Somatic karyotypes of the nine annual species of Cicer (2n = 16) were analyzed using C-banding. Highly significant differences in haploid genome length and C-band positive heterochromatin content were observed. The haploid genome length ranged from 20.0 μm in the wild species C. judaicum to 28.7 μm in the cultiv...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-093

    authors: Tayyar RI,Lukaszewski AJ,Waines JG

    更新日期:1994-08-01 00:00:00

  • RAPD analysis of seed purity in a commercial hybrid cabbage (Brassica oleracea var. capitata) cultivar.

    abstract::The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines wa...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Crockett PA,Bhalla PL,Lee CK,Singh MB

    更新日期:2000-04-01 00:00:00

  • Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris.

    abstract::The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus 'Surpass 400' are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified pr...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g07-103

    authors: Yu F,Lydiate DJ,Rimmer SR

    更新日期:2008-01-01 00:00:00

  • Bacterial diversity in the waterholes of the Kruger National Park: an eDNA metabarcoding approach 1.

    abstract::Bacteria are essential components of natural environments. They contribute to ecosystem functioning through roles as mutualists and pathogens for larger species, and as key components of food webs and nutrient cycles. Bacterial communities respond to environmental disturbances, and the tracking of these communities ac...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0064

    authors: Farrell MJ,Govender D,Hajibabaei M,van der Bank M,Davies TJ

    更新日期:2019-03-01 00:00:00

  • A physical map of the Thinopyrum-derived Lr19 translocation.

    abstract::Twenty-nine lines with deletions in the Lr19 ('Indis') translocated chromosome segment were used to physically map three Thinopyrum RFLP loci as well as the Sr25 and Sd1 loci. From the data, the relative locations of marker loci on the translocation were determined as: Sd1, Xpsr165, Xpsr105, Xps129, Lr19, Wsp-D1, Sr25...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-126

    authors: Prins R,Marais GF,Marais AS,Janse BJ,Pretorius ZA

    更新日期:1996-10-01 00:00:00

  • Evaluating five different loci (rbcL, rpoB, rpoC1, matK, and ITS) for DNA barcoding of Indian orchids.

    abstract::Orchidaceae, one of the largest families of angiosperms, is represented in India by 1600 species distributed in diverse habitats. Orchids are in high demand owing to their beautiful flowers and therapeutic properties. Overexploitation and habitat destruction have made many orchid species endangered. In the absence of ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0215

    authors: Parveen I,Singh HK,Malik S,Raghuvanshi S,Babbar SB

    更新日期:2017-08-01 00:00:00

  • Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population.

    abstract::Fusarium head blight (FHB) is one of the most important fungal wheat diseases worldwide. Understanding the genetics of FHB resistance is the key to facilitating the introgression of different FHB resistance genes into adapted wheat. The objectives of the present study were to detect and map quantitative trait loci (QT...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-104

    authors: Yang Z,Gilbert J,Fedak G,Somers DJ

    更新日期:2005-04-01 00:00:00

  • Construction of a genetic linkage map and QTL analysis in bambara groundnut.

    abstract::Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construc...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0153

    authors: Ahmad NS,Redjeki ES,Ho WK,Aliyu S,Mayes K,Massawe F,Kilian A,Mayes S

    更新日期:2016-07-01 00:00:00

  • Interactions between the scs and Vi genes in alloplasmic durum wheat.

    abstract::Two nuclear genes, vitality (Vi) on an A- or B-genome chromosome and species cytoplasm specific (scs) on a 1DL telosome from Triticum aestivum L. or a telosome from Aegilops uniaristata Vis. (un telosome), improved compatibility between the nucleus of Triticum turgidum L. var. durum and the cytoplasm of Ae. longissima...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-029

    authors: Maan SS

    更新日期:1994-04-01 00:00:00

  • Mitochondrial DNA variation within and among populations of the mosquito Aedes albopictus.

    abstract::A survey of restriction fragment polymorphism in mitochondrial DNA (mtDNA) of 17 populations of the mosquito Aedes albopictus was undertaken. The mtDNA size was estimated to be about 17.5 kbp. The level of polymorphism was low, with over 99% of the fragments being shared in common among the 17 populations. Three popul...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g91-046

    authors: Kambhampati S,Rai KS

    更新日期:1991-04-01 00:00:00

  • Genetic mapping of resistance factors to Phytophthora palmivora in cocoa.

    abstract::Phytophthora palmivora causes pod rot, a serious disease on cocoa widespread throughout the producing regions. In order to ascertain the genetic determination of cocoa resistance to P. palmivora, a study was carried out on two progenies derived from crosses between a heterozygous, moderately resistant Forastero clone,...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-44-1-79

    authors: Flament MH,Kebe I,Clément D,Pieretti I,Risterucci AM,N'Goran JA,Cilas C,Despréaux D,Lanaud C

    更新日期:2001-02-01 00:00:00

  • The first mitochondrial genome for the wasp superfamily Platygastroidea: the egg parasitoid Trissolcus basalis.

    abstract::The nearly complete mitochondrial (mt) genome of an egg parasitoid, Trissolcus basalis (Wollaston), was sequenced using both 454 and Illumina next-generation sequencing technologies. A portion of the noncoding region remained unsequenced, possibly owing to the presence of repeats. The sequenced portion of the genome i...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g2012-005

    authors: Mao M,Valerio A,Austin AD,Dowton M,Johnson NF

    更新日期:2012-03-01 00:00:00

  • New karyotypes of Atlantic tree rats, genus Phyllomys (Rodentia: Echimyidae).

    abstract::Phyllomys (Echimyidae, Rodentia) is a genus of Neotropical rodents with available cytogenetic data restricted to six out of 13 species, mainly based on simple staining methods, without detailed analyses. In this work, we present new karyotypes for Phyllomys lamarum (diploid number 2n = 56, fundamental number or number...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2013-0168

    authors: Araújo NP,Loss AC,Cordeiro-Junior DA,da Silva KR,Leite YL,Svartman M

    更新日期:2014-01-01 00:00:00

  • Recombination nodule mapping and chiasma distribution in spermatocytes of the pigeon, Columba livia.

    abstract::Pigeon spermatocytes were processed with a drying-down technique and their synaptonemal complex (SC) complements were analyzed by electron microscopy. The synaptonemal complex karyotype of the macrobivalents shows an excellent correspondence with the mitotic karyotype. The number and distribution of recombination nodu...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g98-138

    authors: Pigozzi MI,Solari AJ

    更新日期:1999-04-01 00:00:00

  • Varying signals of the effects of natural selection during teleost growth hormone gene evolution.

    abstract::The growth hormone (GH) gene of teleost fish exhibits a higher degree of variability compared with other vertebrate groups. However, the different selective constraints at the sequence level are not well understood. In this study, maximum-likelihood (ML) models of codon substitutions were used to investigate Darwinian...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-079

    authors: Ryynänen HJ,Primmer CR

    更新日期:2006-01-01 00:00:00

  • Transcriptome analysis reveals hybridization-induced genome shock in an interspecific F1 hybrid from Camellia.

    abstract::The combination of two divergent genomes during hybridization can result in "genome shock". Although genome shock has been reported in the hybrids of some herbaceous plants, the pattern and the principle it follows are far from understood, especially in woody plants. Here, the gene expression patterns were remodeled i...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2017-0105

    authors: Zhang M,Liu XK,Fan W,Yan DF,Zhong NS,Gao JY,Zhang WJ

    更新日期:2018-07-01 00:00:00

  • Karyotyping of Neurospora crassa using synaptonemal complex spreads of translocation quadrivalents.

    abstract::The purposes of the present research are (i) to establish the karyotype of Neursopora crassa using visualization of kinetochores in the synaptonemal complex (SC) spreads, (ii) to assign each chromosome to a linkage group, and (iii) to examine chromosome pairing and recombination nodules in quadrivalents. Two strains c...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-008

    authors: Lu BC

    更新日期:2006-06-01 00:00:00

  • Spectral karyotyping (SKY) of mouse meiotic chromosomes.

    abstract::The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identifie...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g01-018

    authors: Heng HH,Liu G,Lu W,Bremer S,Ye CJ,Hughes M,Moens P

    更新日期:2001-04-01 00:00:00

  • AFLP markers linked to resistance against Striga gesnerioides race 1 in cowpea (Vigna unguiculata).

    abstract::Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross b...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-043

    authors: Ouédraogo JT,Tignegre JB,Timko MP,Beizile FJ

    更新日期:2002-10-01 00:00:00

  • Genetic and cytogenetic analyses of the A genome of Triticum monococcum. IX. Cytological behaviour, phenotypic characteristics, breeding behaviour, and fertility of primary, double, and triple trisomics.

    abstract::Cytogenetic studies in Triticum monococcum (2n = 2x = 14, AA) were initiated by generating a series of primary as well as double and triple trisomics from autotriploids derived from crosses between induced autotetraploids and a diploid progenitor. Analysis of meiotic chromosome behaviour revealed that, with the except...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-077

    authors: Kim NS,Kuspira J

    更新日期:1993-06-01 00:00:00

  • Assessment of genetic variation and differentiation of hop genotypes by microsatellite and AFLP markers.

    abstract::Microsatellites have many desirable marker properties and have been increasingly used in crop plants in genetic diversity studies. Here we report on the characterisation of microsatellite markers and on their use for the determination of genetic identities and the assessment of genetic variability among accessions fro...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-44-5-773

    authors: Jakse J,Kindlhofer K,Javornik B

    更新日期:2001-10-01 00:00:00

  • Development of microsatellite markers in Fragaria, their use in genetic diversity analysis, and their potential for genetic linkage mapping.

    abstract::We have developed 21 new microsatellites in the model diploid perennial species Fragaria vesca from an enriched genomic library developed using F. vesca 'Ruegen'. The transferability of the primer pairs to other Fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawber...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-142

    authors: Hadonou AM,Sargent DJ,Wilson F,James CM,Simpson DW

    更新日期:2004-06-01 00:00:00

  • Genome size variation in the genus Avena.

    abstract::Genome size is an indicator of evolutionary distance and a metric for genome characterization. Here, we report accurate estimates of genome size in 99 accessions from 26 species of Avena. We demonstrate that the average genome size of C genome diploid species (2C = 10.26 pg) is 15% larger than that of A genome species...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0132

    authors: Yan H,Martin SL,Bekele WA,Latta RG,Diederichsen A,Peng Y,Tinker NA

    更新日期:2016-03-01 00:00:00