Development of microsatellite markers in Fragaria, their use in genetic diversity analysis, and their potential for genetic linkage mapping.

Abstract:

:We have developed 21 new microsatellites in the model diploid perennial species Fragaria vesca from an enriched genomic library developed using F. vesca 'Ruegen'. The transferability of the primer pairs to other Fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawberry Fragaria x ananassa, whereas 24 (77%) amplified a product in 7 other diploid Fragaria species. We analysed the allelic variation among 15 F. vesca accessions using the 21 microsatellites reported here and 10 F. vesca microsatellites described previously. The level of polymorphism detected at these microsatellite loci was high; five loci were monomorphic. Only two microsatellites were required to unambiguously discriminate among the 15 F. vesca accessions. A preliminary survey of segregation in an F2 progeny indicates that 20 of the 26 polymorphic loci (77%) could be mapped.

journal_name

Genome

journal_title

Genome

authors

Hadonou AM,Sargent DJ,Wilson F,James CM,Simpson DW

doi

10.1139/g03-142

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

429-38

issue

3

eissn

0831-2796

issn

1480-3321

pii

g03-142

journal_volume

47

pub_type

杂志文章

相关文献

GENOME文献大全
  • The mitochondrial genome of Anopheles quadrimaculatus species A: complete nucleotide sequence and gene organization.

    abstract::The complete sequence (15,455 bp) of the mitochondrial DNA of the mosquito Anopheles quadrimaculatus species A is reported. This genome is compact and very A+T rich (77.4% A+T). It contains genes for 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and 13 subunits of the mitochondrial inner membrane respiratory com...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-141

    authors: Mitchell SE,Cockburn AF,Seawright JA

    更新日期:1993-12-01 00:00:00

  • Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment.

    abstract::This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-144

    authors: Nenno M,Schumann K,Nagl W

    更新日期:1994-12-01 00:00:00

  • Primate-specific histone variants.

    abstract::Canonical histones (H2A, H2B, H3, and H4) are present in all eukaryotes where they package genomic DNA and participate in numerous cellular processes, such as transcription regulation and DNA repair. In addition to the canonical histones, there are many histone variants, which have different amino acid sequences, poss...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0094

    authors: Ding D,Nguyen TT,Pang MYH,Ishibashi T

    更新日期:2020-11-27 00:00:00

  • Directional dominance and a developmental model for the expression of the Tda testis-determining autosomal trait of the mouse.

    abstract::The POSCH-2 Y chromosome from the poschiavinus variety of Mus musculus domesticus causes incomplete testis development in the recessive autosomal background of the C57BL/6J laboratory mouse strain. Testis development is normal with the POSCH-2 Y in its native strain background as well as in some strains of the laborat...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-066

    authors: Eales BA,Nahas M,Biddle FG

    更新日期:1996-06-01 00:00:00

  • Analysis of genetic diversity in red clover (Trifolium pratense L.) breeding populations as revealed by RAPD genetic markers.

    abstract::Red clover is an important forage legume species for temperate regions and very little is known about the genetic organization of its breeding populations. We used random amplified polymorphic DNA (RAPD) genetic markers to address the genetic diversity and the distribution of variation in 20 breeding populations and c...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-030

    authors: Ulloa O,Ortega F,Campos H

    更新日期:2003-08-01 00:00:00

  • Repair of base-base mismatches in simian and human cells.

    abstract::Mismatched heteroduplexes arise as intermediates of several dissimilar genetic processes. The outcome of these genetic events will therefore be influenced by the efficiency and specificity of mismatch repair. We have studied the correction of base-base mispairs in simian and human fibroblasts by transfecting the cells...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g89-107

    authors: Brown TC,Jiricny J

    更新日期:1989-01-01 00:00:00

  • Multiple flowering time QTLs within several Brassica species could be the result of duplicated copies of one ancestral gene.

    abstract::Quantitative trait locus (QTL) analysis was used to study the evolution of genes controlling the timing of flowering in four Brassica genomes that are all extensively replicated. Comparative mapping showed that a chromosomal region from the top of Arabidopsis thaliana chromosome 5 corresponded to three homoeologous co...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Axeisson T,Shavorskaya O,Lagercrantz U

    更新日期:2001-10-01 00:00:00

  • A cytogenetic analysis of the Simulium ochraceum species complex (Diptera: Simuliidae) in Central America.

    abstract::A cytobiotaxonomic study of the medically important insect vector Simulium ochraceum s.l. revealed two sibling species and one cytotype from various endemic and nonendemic zones of human onchocerciasis in Guatemala and Mexico. Polytene chromosome maps and idiograms as well as notes on the biology of the three taxa des...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-006

    authors: Hirai H,Procunier WS,Ochoa JO,Uemoto K

    更新日期:1994-02-01 00:00:00

  • First isolation and characterization of Brucella suis from yak.

    abstract::Brucella spp., facultative intracellular pathogens that can persistently colonize animal host cells and cause zoonosis, affect public health and safety. A Brucella strain was isolated from yak in Qinghai Province. To detect whether this isolate could cause an outbreak of brucellosis and to reveal its genetic character...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2019-0101

    authors: Yang X,Wang N,Cao X,Bie P,Xing Z,Yin S,Jiang H,Wu Q

    更新日期:2020-08-01 00:00:00

  • The HtrA2 Drosophila model of Parkinson's disease is suppressed by the pro-survival Bcl-2 Buffy.

    abstract::Mutations in High temperature requirement A2 (HtrA2), also designated PARK13, which lead to the loss of its protease activity, have been associated with Parkinson's disease (PD). HtrA2 is a mitochondrial protease that translocates to the cytosol upon the initiation of apoptosis where it participates in the abrogation ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2016-0069

    authors: M'Angale PG,Staveley BE

    更新日期:2017-01-01 00:00:00

  • Assessment of the genetic diversity and phylogenetic relationships of a temperate bamboo collection by using transferred EST-SSR markers.

    abstract::Polymorphic expressed sequence tag - simple sequence repeat (EST-SSR) markers derived from major cereal crops were used to assess the genetic diversity of the USDA temperate bamboo collection consisting of 92 accessions classified in 11 separate genera and 44 species. A total of 211 bands were detected with a mean num...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-022

    authors: Barkley NA,Newman ML,Wang ML,Hotchkiss MW,Pederson GA

    更新日期:2005-08-01 00:00:00

  • Karyotype evolution in the Pinaceae: implication with molecular phylogeny.

    abstract::The family Pinaceae is made up mostly of diploid species (2n = 24). Systematization of karyotype analysis was developed to make comparison of intra- and interspecific karyotypes among the Pinaceae more accurate and reliable. Considering all parameters, the genera Pseudotsuga and Pseudolarix have the "most derived" (or...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/g2012-061

    authors: Nkongolo KK,Mehes-Smith M

    更新日期:2012-11-01 00:00:00

  • Development and molecular cytogenetic identification of new winter wheat--winter barley ('Martonvásári 9 kr1' - 'Igri') disomic addition lines.

    abstract::This paper describes a series of winter wheat - winter barley disomic addition lines developed from hybrids between winter wheat line Triticum aestivum L. 'Martonvásári 9 kr1' and the German 2-rowed winter barley cultivar Hordeum vulgare L. 'Igri'. The barley chromosomes in a wheat background were identified from the ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-134

    authors: Szakács E,Molnár-Láng M

    更新日期:2007-01-01 00:00:00

  • Molecular differentiation of two sibling species of the black fly Simulium vittatum (Diptera: Simuliidae) based on random amplified polymorphic DNA.

    abstract::Larvae of the black fly morphospecies Simulium vittatum from Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with 33P, and run on polyacrylamide gels. The entire da...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-144

    authors: Duncan GA,Adler PH,Pruess KP,Powers TO

    更新日期:2004-04-01 00:00:00

  • Karyotyping of Neurospora crassa using synaptonemal complex spreads of translocation quadrivalents.

    abstract::The purposes of the present research are (i) to establish the karyotype of Neursopora crassa using visualization of kinetochores in the synaptonemal complex (SC) spreads, (ii) to assign each chromosome to a linkage group, and (iii) to examine chromosome pairing and recombination nodules in quadrivalents. Two strains c...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-008

    authors: Lu BC

    更新日期:2006-06-01 00:00:00

  • Three new satellite sequences and a mobile element found inside HSP70 introns of the Mediterranean mussel (Mytilus galloprovincialis).

    abstract::We report the characterization of 3 new repetitive sequences from the bivalve mollusc Mytilus galloprovincialis, designated Mg1, Mg2, and Mg3, with monomer lengths of 169, 260, and 70 bp, respectively. The 3 repeats together constitute approximately 7.8% of the M. galloprovincialis genome and were found, together with...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-111

    authors: Kourtidis A,Drosopoulou E,Pantzartzi CN,Chintiroglou CC,Scouras ZG

    更新日期:2006-11-01 00:00:00

  • Presence of a centromeric filament during meiosis.

    abstract::Spermatocytes at meiotic metaphase I and anaphase I have a characteristic centromeric filament in a variety of vertebrate organisms. This centromeric filament was first demonstrated on mouse spermatocytes and its presence is now extended to spermatocytes from the human, rat, golden hamster, bull, and chicken. The visu...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g91-136

    authors: Solari AJ,Tandler CJ

    更新日期:1991-12-01 00:00:00

  • Simple sequence repeats in Cucumis mapping and map merging.

    abstract::Thirty-four polymorphic simple-sequence repeats (SSRs) were evaluated for length polymorphism in melon (Cucumis melo L.) and cucumber (Cucumis sativus L.). SSR markers were located on three melon maps (18 on the map of 'Vedrantais' and PI 161375, 23 on the map of 'Piel de Sapo' and PI 161375, and 16 on the map of PI 4...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Danin-Poleg Y,Reis N,Baudracco-Arnas S,Pitrat M,Staub JE,Oliver M,Arus P,deVicente CM,Katzir N

    更新日期:2000-12-01 00:00:00

  • Multiple supernumerary chromosomes in the pseudogamous parthenogenetic flatworm Polycelis nigra: lineage markers or remnants of genetic leakage?

    abstract::Polycelis nigra is a free-living simultaneous hermaphroditic flatworm that has amphimictic and pseudogamous parthenogenetic biotypes. Sexual individuals are always diploid (2n = 16) and pseudogamous parthenogens are polyploid (usually triploid). Two types of supernumerary chromosomes are found in parthenogens, those r...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-810

    authors: Sharbel TF,Beukeboom LW,Pijnacker LP

    更新日期:1997-12-01 00:00:00

  • Molecular changes at Rrn loci in barley (Hordeum vulgare L.) hybrids with H. bulbosum (L.)

    abstract::Southern blots of restriction fragments of genomic DNAs from Hordeum vulgare (L.), H. bulbosum (L.), and interspecific hybrids and their derivatives were hybridized with rDNA probe to identify locus-specific modifications at Rrn loci. H. bulbosum rDNA revealed a single EcoRV site per repeat compared with two sites in ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g99-053

    authors: Kranthi Kumar P,Subrahmanyam NC

    更新日期:1999-12-01 00:00:00

  • Development of a PCR-based allele-specific assay from an RFLP probe linked to resistance to cereal cyst nematode in wheat.

    abstract::The RFLP locus Xglk605 identified by the probe Tag605 maps to a proximal position on the long arm of wheat chromosome 2B about 7 cM away from a gene conditioning resistance to cereal cyst nematode in the wheat line AUS10894. The clone Tag605 was partially sequenced and the PCR primer set AWP1 was designed. The 292-bp ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-100

    authors: Williams KJ,Fisher JM,Langridge P

    更新日期:1996-08-01 00:00:00

  • Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n = 28).

    abstract::Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was car...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-023

    authors: Yasukochi Y,Banno Y,Yamamoto K,Goldsmith MR,Fujii H

    更新日期:2005-08-01 00:00:00

  • Genome flux in tomato cell clones cultured in vitro in different physiological equilibria. II. A RAPD analysis of variability.

    abstract::An analysis of the effect of changing physiological conditions on genome evolution in tomato cell populations has been carried out on long-term in vitro cultured clones grown on different auxin-cytokinin equilibria or selected for low-high competence for active defense against Fusarium oxysporum f.sp. lycopersici. RAP...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-107

    authors: Bogani P,Simoni A,Lio' P,Scialpi A,Buiatti M

    更新日期:1996-10-01 00:00:00

  • A minisatellite polymorphism in intron III of the barramundi (Lates calcarifer) growth hormone gene.

    abstract::This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-117

    authors: Yowe DL,Epping RJ

    更新日期:1996-10-01 00:00:00

  • Size matters in Triticeae polyploids: larger genomes have higher remodeling.

    abstract::Polyploidization is one of the major driving forces in plant evolution and is extremely relevant to speciation and diversity creation. Polyploidization leads to a myriad of genetic and epigenetic alterations that ultimately generate plants and species with increased genome plasticity. Polyploids are the result of the ...

    journal_title:Genome

    pub_type: 杂志文章,评审

    doi:10.1139/G10-107

    authors: Bento M,Gustafson JP,Viegas W,Silva M

    更新日期:2011-03-01 00:00:00

  • Fluorescent in situ hybridization and C-banding analyses of highly repetitive DNA sequences in the heterochromatin of rye (Secale montanum Guss.) and wheat incorporating S. montanum chromosome segments.

    abstract::The molecular characterization of C-banded regions of Secale montanum Guss. by means of in situ hybridization was performed in order to provide new information about their chromosome structure relative to cultivated rye, Secale cereale L. Accurate identification of individual chromosomes was achieved using simultaneou...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-101

    authors: Cuadrado A,Jouve N

    更新日期:1995-08-01 00:00:00

  • Bacterial diversity in the waterholes of the Kruger National Park: an eDNA metabarcoding approach 1.

    abstract::Bacteria are essential components of natural environments. They contribute to ecosystem functioning through roles as mutualists and pathogens for larger species, and as key components of food webs and nutrient cycles. Bacterial communities respond to environmental disturbances, and the tracking of these communities ac...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2018-0064

    authors: Farrell MJ,Govender D,Hajibabaei M,van der Bank M,Davies TJ

    更新日期:2019-03-01 00:00:00

  • Sequence analysis of single-copy genes in two wild olive subspecies: nucleotide diversity and potential use for testing admixture.

    abstract::The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspec...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2014-0001

    authors: Besnard G,El Bakkali A

    更新日期:2014-03-01 00:00:00

  • A single molecular marker to distinguish between species of Dioscorea.

    abstract::Yams are species of the genus Dioscorea (family Dioscoreaceae), which consists of approximately 630 species. The majority of the world production of yams occurs in Africa with 58.8 million t annually, but they are also produced in the Americas and Asia. The saponins in yams have been reported to possess various proper...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2015-0105

    authors: Techen N,Parveen I,Khan IA

    更新日期:2017-03-01 00:00:00

  • Isochromosome Y in an infertile heifer.

    abstract::The chromosome makeup of a 3-year-old crossbred heifer was examined in an attempt to explore the cytogenetic causes of her infertility. The heifer proved to be a mosaic carrying predominantly XY cells and with approximately 30% of cells lacking a normal Y. The minor population included cells carrying a dicentric isoch...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g90-104

    authors: Pinheiro LE,Mikich AB,Bechara GH,Almeida IL,Basrur PK

    更新日期:1990-10-01 00:00:00