Abstract:
:We have developed 21 new microsatellites in the model diploid perennial species Fragaria vesca from an enriched genomic library developed using F. vesca 'Ruegen'. The transferability of the primer pairs to other Fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawberry Fragaria x ananassa, whereas 24 (77%) amplified a product in 7 other diploid Fragaria species. We analysed the allelic variation among 15 F. vesca accessions using the 21 microsatellites reported here and 10 F. vesca microsatellites described previously. The level of polymorphism detected at these microsatellite loci was high; five loci were monomorphic. Only two microsatellites were required to unambiguously discriminate among the 15 F. vesca accessions. A preliminary survey of segregation in an F2 progeny indicates that 20 of the 26 polymorphic loci (77%) could be mapped.
journal_name
Genomejournal_title
Genomeauthors
Hadonou AM,Sargent DJ,Wilson F,James CM,Simpson DWdoi
10.1139/g03-142subject
Has Abstractpub_date
2004-06-01 00:00:00pages
429-38issue
3eissn
0831-2796issn
1480-3321pii
g03-142journal_volume
47pub_type
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