Fluorescent in situ hybridization and C-banding analyses of highly repetitive DNA sequences in the heterochromatin of rye (Secale montanum Guss.) and wheat incorporating S. montanum chromosome segments.

Abstract:

:The molecular characterization of C-banded regions of Secale montanum Guss. by means of in situ hybridization was performed in order to provide new information about their chromosome structure relative to cultivated rye, Secale cereale L. Accurate identification of individual chromosomes was achieved using simultaneous and (or) successive fluorescent in situ hybridization (FISH) and C-banding. FISH identification was performed using total rye DNA, three highly repetitive rye DNA sequences (pSc119.2, pSc74, and pSc34), and the ribosomal RNA probes pTa71 (18S, 5.8S, and 26S rDNA) and pTa794 (5S rDNA). FISH was also used to identify the chromosome segment involved in two spontaneous translocation lines recovered from a 'Chinese Spring'--S. montanum wheat-rye addition line. FISH analysis revealed the exact translocation breakpoints and allowed the identification of the transferred rye segments. The value of this type of analysis is discussed.

journal_name

Genome

journal_title

Genome

authors

Cuadrado A,Jouve N

doi

10.1139/g95-101

subject

Has Abstract

pub_date

1995-08-01 00:00:00

pages

795-802

issue

4

eissn

0831-2796

issn

1480-3321

journal_volume

38

pub_type

杂志文章

相关文献

GENOME文献大全
  • Identification of a major locus conferring resistance to powdery mildew (Erysiphe polygoni DC) in mungbean (Vigna radiata L. Wilczek) by QTL analysis.

    abstract::A major locus conferring resistance to the causal organism of powdery mildew, Erysiphe polygoni DC, in mungbean (Vigna radiata L. Wilczek) was identified using QTL analysis with a population of 147 recombinant inbred individuals. The population was derived from a cross between 'Berken', a highly susceptible variety, a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-057

    authors: Humphry ME,Magner T,McIntyre CL,Aitken EA,Liu CJ

    更新日期:2003-10-01 00:00:00

  • Isolation of a new species-specific repetitive sequence from Thinopyrum elongatum and its use in the studies of alien translocations.

    abstract::A new repetitive sequence that is extremely abundant and well dispersed in the Thinopyrum elongatum genome but present in low-copy number in wheat (Triticum aestivum L.) has been isolated. This repeat and a Th. elongatum repeat isolated in another laboratory were used to identify cosmid genomic clones containing the r...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g94-012

    authors: Bournival B,Obanni M,Abad A,Ohm H,Mackenzie S

    更新日期:1994-02-01 00:00:00

  • Rapid detection of 1B, 1BL/1RS heterozygotes in the development of homozygous 1BL/1RS translocation stocks of Triticum turgidum (2n = 4x = 28).

    abstract::A biochemical marker was utilized to facilitate detection of chromosome 1B, 1BL/1RS translocation heterozygote plants in segregating backcross progenies during the development of 1BL/1RS homozygous lines in several Triticum turgidum L. cultivars (2n = 4x = 28; AABB). Isoelectric focussing of glucose phosphate isomeras...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-144

    authors: William MD,Mujeeb-Kazi A

    更新日期:1993-12-01 00:00:00

  • First isolation and characterization of Brucella suis from yak.

    abstract::Brucella spp., facultative intracellular pathogens that can persistently colonize animal host cells and cause zoonosis, affect public health and safety. A Brucella strain was isolated from yak in Qinghai Province. To detect whether this isolate could cause an outbreak of brucellosis and to reveal its genetic character...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2019-0101

    authors: Yang X,Wang N,Cao X,Bie P,Xing Z,Yin S,Jiang H,Wu Q

    更新日期:2020-08-01 00:00:00

  • Haplotype characterization and markers at the barley Mlo powdery mildew resistance locus as tools for marker-assisted selection.

    abstract::Recessive mlo alleles of the barley Mlo gene confer resistance to almost all known isolates of the powdery mildew fungal pathogen targeting barley (Hordeum vulgare). To characterize haplotypes present in the Mlo chromosomal region of cultivated Mlo and mlo barley genotypes, we conducted a polymorphism search in 3 pred...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-044

    authors: Tacconi G,Baldassarre V,Collins NC,Bulgarelli D,Stanca AM,Valè G

    更新日期:2006-08-01 00:00:00

  • An AFLP marker tightly linked to apomixis reveals hemizygosity in a portion of the apomixis-controlling locus in Paspalum simplex.

    abstract::A mapping population of Paspalum simplex segregating for apomixis (asexual reproduction through seeds) was screened with AFLPs to find apomixis-linked markers. Four AFLPs linked to apomixis in coupling phase were found. Three of them did not show recombinants among the 87 individuals of the mapping population, whereas...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-014

    authors: Labombarda P,Busti A,Caceres ME,Pupilli F,Arcioni S

    更新日期:2002-06-01 00:00:00

  • A study of homologous chromosomes using a morphometric approach.

    abstract::A previous study of 100 karyotyped metaphase cells has demonstrated the utility of a graphic arts tool in deriving chromosome measurements for relative length determination. In the present study we utilize this same approach to address the question: "What are the average differences in relative lengths between apparen...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-132

    authors: Mark HF,Wyandt HE,Pan T,Mark R Jr,Paramenter M,Campbell W,Mark Y

    更新日期:1993-10-01 00:00:00

  • Full-length transcriptome analysis and identification of genes involved in asarinin and aristolochic acid biosynthesis in medicinal plant Asarum sieboldii.

    abstract::Asarum sieboldii a well-known traditional Chinese medicinal herb, is used for curing inflammation and ache. It contains both bioactive ingredient asarinin and the toxic compound aristolochic acid. To address further breeding demand, genes involved in the biosynthetic pathways of asarinin and aristolochic acid should b...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0095

    authors: Chen C,Shi X,Zhou T,Li W,Li S,Bai G

    更新日期:2020-12-15 00:00:00

  • Chloroplast microsatellite polymorphisms in Vitis species.

    abstract::The use of consensus chloroplast microsatellites primers for dicotyledonous chloroplast genomes revealed the existence of intra and interspecific length variation within the genus Vitis. Three chloroplast microsatellite loci were found to be polymorphic in samples of Vitis vinifera, Vitis berlandieri, Vitis riparia, a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g02-087

    authors: Arroyo-García R,Lefort F,de Andrés MT,Ibáñaez J,Borrego J,Jouve N,Cabello F,Martínez-Zapater JM

    更新日期:2002-12-01 00:00:00

  • Identification of enhancers that drive the spatially restricted expression of Vsx1 and Rx in the outer proliferation center of the developing Drosophila optic lobe.

    abstract::Combinatorial spatial and temporal patterning of stem cells is a powerful mechanism for the generation of neural diversity in insect and vertebrate nervous systems. In the developing Drosophila medulla, the neural stem cells of the outer proliferation center (OPC) are spatially patterned by the mutually exclusive expr...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0034

    authors: Islam IM,Ng J,Valentino P,Erclik T

    更新日期:2021-02-01 00:00:00

  • Cytogenetics and characterization of microsatellite loci for a South American pioneer tree species, Croton floribundus.

    abstract::Despite the recent advances in plant population genetic studies, the lack of information regarding pedigree, ploidy level, or mode of inheritance for many polyploids can compromise the analysis of the molecular data produced. The aim of this study was to examine both microsatellite and cytogenetic characteristics of t...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2013-0159

    authors: Silvestrini M,Pinto-Maglio CA,Zucchi MI,dos Santos FA

    更新日期:2013-12-01 00:00:00

  • Relationship between pachytene synapsis, metaphase I associations, and transmission of 2B and 4B chromosomes in rye.

    abstract::2B rye plants selected for high (H) or low (L) B transmission rate were studied at pachytene and metaphase I of meiosis to determine the relationship between synapsis, bivalents at metaphase I, and B transmission rate. The results show that the 2 B chromosomes (Bs) form bivalents at pachytene in both the H and L lines...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Jiménez G,Manzanero S,Puertas MJ

    更新日期:2000-04-01 00:00:00

  • Somatic chimerism, genetic inheritance, and mapping of the fleshless berry (flb) mutation in grapevine (Vitis vinifera L.).

    abstract::The fleshless berry (flb) mutation of grapevine (Vitis vinifera L. 'Ugni Blanc') impairs the differentiation and division of inner mesocarp cells responsible for flesh in grapevine berries. In order to study the inheritance of the mutation and to map the flb locus, 5 segregating populations were created. Progeny plant...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-034

    authors: Fernandez L,Doligez A,Lopez G,Thomas MR,Bouquet A,Torregrosa L

    更新日期:2006-07-01 00:00:00

  • Efficiency of RFLP, RAPD, and AFLP markers for the construction of an intraspecific map of the tomato genome.

    abstract::We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment le...

    journal_title:Genome

    pub_type: 杂志文章

    doi:

    authors: Saliba-Colombani V,Causse M,Gervais L,Philouze J

    更新日期:2000-02-01 00:00:00

  • Standard karyotype of Triticum longissimum and its cytogenetic relationship with T. aestivum.

    abstract::C-banding polymorphism was analyzed in 17 accessions of Triticum longissimum from Israel and Jordan, and a generalized idiogram of this species was established. C-banding analysis was further used to identify two sets of disomic T. aestivum - T. longissimum chromosome addition lines and 13 ditelosomic addition lines a...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g93-098

    authors: Friebe B,Tuleen N,Jiang J,Gill BS

    更新日期:1993-08-01 00:00:00

  • Identifying a single-copy DNA sequence associated with the expression of a heterochromatic gene, the light locus of Drosophila melanogaster.

    abstract::Although little is known about the molecular organization of most genes within heterochromatin, the unusual properties of these chromosomal regions suggest that genes therein may be organized and expressed very differently from those in euchromatin. We report here the cloning, by P transposon tagging, of sequences ass...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g90-062

    authors: Devlin RH,Holm DG,Morin KR,Honda BM

    更新日期:1990-06-01 00:00:00

  • Genetic control and identification of QTLs associated with visual quality traits of field pea (Pisum sativum L.).

    abstract::Visual quality of field pea (Pisum sativum L.) is one of the most important determinants of the market value of the harvested crop. Seed coat color, seed shape, and seed dimpling are the major components of visual seed quality of field pea and are considered as important breeding objectives. The objectives of this res...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g10-117

    authors: Ubayasena L,Bett K,Tar'an B,Warkentin T

    更新日期:2011-04-01 00:00:00

  • Varying signals of the effects of natural selection during teleost growth hormone gene evolution.

    abstract::The growth hormone (GH) gene of teleost fish exhibits a higher degree of variability compared with other vertebrate groups. However, the different selective constraints at the sequence level are not well understood. In this study, maximum-likelihood (ML) models of codon substitutions were used to investigate Darwinian...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-079

    authors: Ryynänen HJ,Primmer CR

    更新日期:2006-01-01 00:00:00

  • The orphan nuclear receptor DHR38 influences transcription of the DOPA decarboxylase gene in epidermal and neural tissues of Drosophila melanogaster.

    abstract::The DOPA decarboxylase gene (Ddc) belongs to the "early-late" class of ecdysone-inducible genes in Drosophila melanogaster. Its expression is up-regulated in epidermal tissues by the ecdysone receptor acting through a response element, EcRE. In this paper, we show that another member of the nuclear receptor superfamil...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g07-084

    authors: Davis MM,Yang P,Chen L,O'Keefe SL,Hodgetts RB

    更新日期:2007-11-01 00:00:00

  • Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique.

    abstract::For molecular and cytogenetic studies, two partial bacterial artificial chromosome (BAC) libraries of the garlic cultivar Allium sativum L. 'Danyang' were constructed using high molecular weight (HMW) garlic DNA, the pBAC1-SACB1 vector, and the pIndigoBAC536 vector. The average insert size of the BAC library was about...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-012

    authors: Lee HR,Eom EM,Lim YP,Bang JW,Lee DH

    更新日期:2003-06-01 00:00:00

  • Directional dominance and a developmental model for the expression of the Tda testis-determining autosomal trait of the mouse.

    abstract::The POSCH-2 Y chromosome from the poschiavinus variety of Mus musculus domesticus causes incomplete testis development in the recessive autosomal background of the C57BL/6J laboratory mouse strain. Testis development is normal with the POSCH-2 Y in its native strain background as well as in some strains of the laborat...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g96-066

    authors: Eales BA,Nahas M,Biddle FG

    更新日期:1996-06-01 00:00:00

  • FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor.

    abstract::Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g97-063

    authors: Gómez MI,Islam-Faridi MN,Woo SS,Czeschin D Jr,Zwick MS,Stelly DM,Price HJ,Schertz KF,Wing RA

    更新日期:1997-08-01 00:00:00

  • AFLP targeting of the 1-cM region conferring the vrs1 gene for six-rowed spike in barley, Hordeum vulgare L.

    abstract::Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g04-073

    authors: He C,Sayed-Tabatabaei BE,Komatsuda T

    更新日期:2004-12-01 00:00:00

  • Polytene chromosome relationships of five species of the Anopheles dirus complex in Thailand.

    abstract::Photographic maps and rearrangements of each salivary gland polytene chromosome arm of Anopheles nemophilous (species F) and of An. dirus species A, B, C, and D of the Dirus group from natural populations in Thailand are presented. Structural conformation of heterokaryotypes and comparison of chromosome banding sequen...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-056

    authors: Poopittayasataporn A,Baimai V

    更新日期:1995-06-01 00:00:00

  • Isolation and characterization of 70 novel microsatellite markers from ostrich (Struthio camelus) genome.

    abstract::Microsatellite markers are widely used in linkage mapping, parentage testing, population genetic studies, and molecular evolution studies in many agricultural species, while only a limited number of ostrich (Struthio camelus) microsatellites have been isolated. Thus, we constructed a random small-insert genomic librar...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g03-059

    authors: Tang B,Huang YH,Lin L,Hu XX,Feng JD,Yao P,Zhang L,Li N

    更新日期:2003-10-01 00:00:00

  • Analysis of QTLs for yield components, agronomic traits, and disease resistance in an advanced backcross population of spring barley.

    abstract::Hordeum vulgare subsp. spontaneum, the wild progenitor of barley, is a potential source of useful genetic variation for barley breeding programs. The objective of this study was to map quantitative trait loci (QTLs) in an advanced backcross population of barley. A total of 207 BC3 lines were developed using the 2-rowe...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g05-128

    authors: Li JZ,Huang XQ,Heinrichs F,Ganal MW,Röder MS

    更新日期:2006-05-01 00:00:00

  • Development of RNA-seq-based molecular markers for characterizing Thinopyrum bessarabicum and Secale introgressions in wheat.

    abstract::Sequence-based markers have added a new dimension in the efficiency of identifying alien introgressions in wheat. Expressed sequence tag-sequence tagged sites (EST-STS) markers have proved useful in tracing alien chromatin. In this study, we report the development of Thinopyrum bessarabicum- and Secale anatolicum-spec...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/gen-2020-0057

    authors: Singh AK,Lo K,Dong C,Zhang P,Trethowan RM,Sharp PJ

    更新日期:2020-11-01 00:00:00

  • Phenetic relationships among different races of Striga gesnerioides (Willd.) Vatke from West Africa.

    abstract::Striga gesnerioides is a root hemiparasite that primarily parasitizes dicotyledonous species, including cowpea (Vigna unguiculata L.) and other legumes. Based on the differential resistance response of various cultivars, landraces, and breeding lines, it has been proposed that several distinct races of cowpea-parasiti...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g06-086

    authors: Botanga CJ,Timko MP

    更新日期:2006-11-01 00:00:00

  • Development of genetic research in the USSR.

    abstract::Two periods of the development of genetic research in the USSR with reference to its current trends of plant and animal genetics, cytogenetics, and molecular genetics are reviewed. A short list of priority areas is established: the maintenance and use of unique gene pools of plants and animals; the domestication of an...

    journal_title:Genome

    pub_type: 历史文章,杂志文章

    doi:10.1139/g89-160

    authors: Shumny VK

    更新日期:1989-01-01 00:00:00

  • Construction of a genetic linkage map in celery using DNA-based markers.

    abstract::A F2 population of two celery cultivated types (Apium graveolens L. var. rapaceum and A. graveolens L. var. secalinum) was used to construct a linkage map consisting of 29 RFLP (restriction fragment length polymorphism), 100 RAPD (random amplified polymorphic DNA), four isozyme, one disease resistance, and one growth ...

    journal_title:Genome

    pub_type: 杂志文章

    doi:10.1139/g95-005

    authors: Yang X,Quiros CF

    更新日期:1995-02-01 00:00:00