Abstract:
:Combinatorial spatial and temporal patterning of stem cells is a powerful mechanism for the generation of neural diversity in insect and vertebrate nervous systems. In the developing Drosophila medulla, the neural stem cells of the outer proliferation center (OPC) are spatially patterned by the mutually exclusive expression of three homeobox transcription factors: Vsx1 in the center of the OPC crescent (cOPC), Optix in the main arms (mOPC), and Rx in the posterior tips (pOPC). These spatial factors act together with a temporal cascade of transcription factors in OPC neuroblasts to specify the greater than 80 medulla cell types. Here, we identify the enhancers that are sufficient to drive the spatially restricted expression of the Vsx1 and Rx genes in the OPC. We show that removal of the cOPC enhancer in the Muddled inversion mutant leads to the loss of Vsx1 expression in the cOPC. Analysis of the evolutionarily conserved sequences within these enhancers suggests that direct repression by Optix may restrict the expression of Vsx1 and Rx to the cOPC and pOPC, respectively.
journal_name
Genomejournal_title
Genomeauthors
Islam IM,Ng J,Valentino P,Erclik Tdoi
10.1139/gen-2020-0034subject
Has Abstractpub_date
2021-02-01 00:00:00pages
109-117issue
2eissn
0831-2796issn
1480-3321journal_volume
64pub_type
杂志文章相关文献
GENOME文献大全abstract::The complete 16 844 bp mitochondrial genome of Neofelis nebulosa has been sequenced and compared with the complete mitochondrial genomes of Felis catus and the Acinonyx jubatus. The base composition of the mitochondrial genome of N. nebulosa is as follows: A, 5343 bp (31.7%); C, 4441 bp (26.4%); G, 2491 bp (14.8%); T,...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-143
更新日期:2007-02-01 00:00:00
abstract::Globally, biodiversity is declining as a result of anthropogenic pressures, and this could lead to extinction of some species before they are discovered. The loss of insect taxa is of prime concern, given recent reports of significant declines in the populations of many taxa across the globe. Efforts to document biodi...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2019-0193
更新日期:2020-11-10 00:00:00
abstract::The genomic origin of alien chromosomes present in a wheat-Thinopyrum intermedium partial amphiploid TAF46 (2n = 8x = 56) and six derived chromosome addition lines were analyzed by genomic in situ hybridization (GISH) using S genomic DNA from Pseudoroegneria strigosa (2n = 2x = 14, SS) as a probe. The GISH analysis cl...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g99-090
更新日期:1999-12-01 00:00:00
abstract::We have characterized a so-called D genome specific repetitive DNA sequence (pAs1) of Aegilops squarrosa L. (2n = 14, genome DD) with respect to its DNA sequence and its distribution among Triticeae species. The clone consisted of three units of a repetitive DNA sequence of 336 or 337 base pairs, and was AT rich (65.2...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-063
更新日期:1995-06-01 00:00:00
abstract::Despite being a controversial crop, Cannabis sativa L. has a long history of cultivation throughout the world. Following recent legalisation in Canada, it is emerging as an important plant for both medicinal and recreational purposes. Recent progress in genome sequencing of both cannabis and hemp varieties allows for ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0087
更新日期:2020-11-13 00:00:00
abstract::The RFLP locus Xglk605 identified by the probe Tag605 maps to a proximal position on the long arm of wheat chromosome 2B about 7 cM away from a gene conditioning resistance to cereal cyst nematode in the wheat line AUS10894. The clone Tag605 was partially sequenced and the PCR primer set AWP1 was designed. The 292-bp ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-100
更新日期:1996-08-01 00:00:00
abstract::A key breeding objective in oat (Avena sativa L.) is cultivars with high and low beta-glucan content. In a targeted strategy to develop PCR-based markers linked to published beta-glucan content quantitative trait loci (QTLs) regions, 15 random amplified polymorphic DNA (RAPD) fragments were cloned and their sequences ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-026
更新日期:2008-06-01 00:00:00
abstract::We have used immunocytochemistry and molecular cloning methods to identify and characterize structural polypeptides of the centromere. These studies permit us to resolve two distinct regions: the inner and outer centromere. (i) Components of the outer centromere: autoantibodies from certain patients with rheumatic dis...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/g89-103
更新日期:1989-01-01 00:00:00
abstract::Thermosensitive genic male-sterile (TGMS) rice is widely used in hybrid rice production. Because of its specific temperature requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered TGMS line, G20S, has an opposite phenotype compared with normal TGMS lines. G20S is completely sterile ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-092
更新日期:2010-02-01 00:00:00
abstract::Phyllomys (Echimyidae, Rodentia) is a genus of Neotropical rodents with available cytogenetic data restricted to six out of 13 species, mainly based on simple staining methods, without detailed analyses. In this work, we present new karyotypes for Phyllomys lamarum (diploid number 2n = 56, fundamental number or number...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2013-0168
更新日期:2014-01-01 00:00:00
abstract::European hazelnut (Corylus avellana L.) is the only economically important nut crop in the family Betulaceae. Because of its small genome size (~385 Mb / 1C), relatively short life cycle, availability of a dense linkage map, and amenability to transformation by Agrobacterium, the European hazelnut could serve as a mod...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g11-048
更新日期:2011-10-01 00:00:00
abstract::The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reac...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-034
更新日期:1996-04-01 00:00:00
abstract::RNA-Seq methodology has been used to generate a comprehensive transcriptome sequence resource for perennial ryegrass, an important temperate pasture grass species. A total of 931 547 255 reads were obtained from libraries corresponding to 19 distinct tissue samples, including both vegetative and reproductive stages of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0077
更新日期:2017-12-01 00:00:00
abstract::The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines wa...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Striga gesnerioides is a root hemiparasite that primarily parasitizes dicotyledonous species, including cowpea (Vigna unguiculata L.) and other legumes. Based on the differential resistance response of various cultivars, landraces, and breeding lines, it has been proposed that several distinct races of cowpea-parasiti...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-086
更新日期:2006-11-01 00:00:00
abstract::An F2 progeny of maritime pine (Pinus pinaster Aïton) was used to investigate the mode of inheritance of δ3-carene using a quantitative and a qualitative approach. A previously reported genetic map constructed with random amplified polymorphic DNA (RAPD) markers made it possible to locate one major quantitative ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-141
更新日期:1996-12-01 00:00:00
abstract::Multiple reaction monitoring mass spectrometry (MRM-MS) is an emerging technology for blood biomarker verification and validation; however, the results may be influenced by pre-analytical factors. This exploratory study was designed to determine if differences in phlebotomy techniques would significantly affect the ab...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0036
更新日期:2015-12-01 00:00:00
abstract::A recent investigation, using a human genomic probe, has indicated that the 68,000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p less than 0.0001). In addition, we have fou...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g88-083
更新日期:1988-08-01 00:00:00
abstract::Variation in recombinant frequencies at two adjacent intervals on chromosome 6 of tomato (Lycopersicon esculentum Mill.) has been studied in seven lines that differ in the amount and origin of introgressed segments from wild species. These lines were all crossed to a genotype homozygous recessive for the markers tl, y...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-062
更新日期:1996-06-01 00:00:00
abstract::Paenibacillus terrae NK3-4 is a plant growth-promoting rhizobacterium that may be useful for controlling plant diseases. We conducted a genomic analysis and identified the genes mediating antimicrobial functions. Additionally, an extracellular antifungal protein component was isolated and identified. The draft genome ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0113
更新日期:2018-10-01 00:00:00
abstract::Genetic parameters were estimated on growth and development traits using analyses of variance and covariance of 42 full-sib families from a select line, 39 full-sib families from a control line of the same strain, and 37 full-sib families from another unselected strain. The traits included percent 1+ smolts, percent s...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g92-046
更新日期:1992-04-01 00:00:00
abstract::C-banding polymorphism was analyzed in 17 accessions of Triticum longissimum from Israel and Jordan, and a generalized idiogram of this species was established. C-banding analysis was further used to identify two sets of disomic T. aestivum - T. longissimum chromosome addition lines and 13 ditelosomic addition lines a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-098
更新日期:1993-08-01 00:00:00
abstract::A previous study of 100 karyotyped metaphase cells has demonstrated the utility of a graphic arts tool in deriving chromosome measurements for relative length determination. In the present study we utilize this same approach to address the question: "What are the average differences in relative lengths between apparen...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-132
更新日期:1993-10-01 00:00:00
abstract::Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was car...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-023
更新日期:2005-08-01 00:00:00
abstract::Unisexual Ambystoma are the oldest known unisexual vertebrates and comprise a lineage of eastern North American all female salamanders that reproduce by stealing sperm from as many as five normally bisexual congeneric species. The sperm may be used to only stimulate egg development by gynogenesis but can be incorporat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2019-0034
更新日期:2019-08-01 00:00:00
abstract::A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshif...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-042
更新日期:2005-10-01 00:00:00
abstract::In the silkworm Bombyx mori, non-susceptibility to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is located on chromosome 15. Owing to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progeny were used for...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-125
更新日期:2006-04-01 00:00:00
abstract::Fluorescent in situ hybridization (FISH) of a 130 kilobase cotton (Gossypium hirsuitum L.) bacterial artificial chromosome (BAC) clone containing a high proportion of single-copy DNA produced a large pair of FISH signals on the distal end of the long arm of a pair of chromosomes of the D-genome species G. raimondii Ul...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-082
更新日期:1995-08-01 00:00:00
abstract::Bacteria are essential components of natural environments. They contribute to ecosystem functioning through roles as mutualists and pathogens for larger species, and as key components of food webs and nutrient cycles. Bacterial communities respond to environmental disturbances, and the tracking of these communities ac...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0064
更新日期:2019-03-01 00:00:00
abstract::The aplocheiloid killifish Rivulus marmoratus is the only known self-fertilizing hermaphroditic vertebrate. Most natural populations consist almost entirely of hermaphrodites and comprise arrays of homozygous clones. However, in almost all populations thus far studied, clonal variation, as detected with molecular tech...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-121
更新日期:1997-12-01 00:00:00