Abstract:
:We have characterized a so-called D genome specific repetitive DNA sequence (pAs1) of Aegilops squarrosa L. (2n = 14, genome DD) with respect to its DNA sequence and its distribution among Triticeae species. The clone consisted of three units of a repetitive DNA sequence of 336 or 337 base pairs, and was AT rich (65.2%). DNA analyses revealed the presence of the pAs1-like sequences in other genomes of Triticeae species, although the repetition was greatly (as much as 100-fold) variable among the genomes. The repetitive sequences from 10 diploid species were amplified using PCR with specific primers, and the sequential variability was analyzed by the digestion pattern obtained with five restriction enzymes. Since the AfaI site was the most conservatively present in the unit of the repetitive sequences, we named them "Afa family." The analysis clearly displayed the variation of the repetitive sequences regardless of the uniformity of the size of the amplified product. These results indicated that plural amplification events of these repetitive sequences happened independently in the genome evolution of Triticeae.
journal_name
Genomejournal_title
Genomeauthors
Nagaki K,Tsujimoto H,Isono K,Sasakuma Tdoi
10.1139/g95-063subject
Has Abstractpub_date
1995-06-01 00:00:00pages
479-86issue
3eissn
0831-2796issn
1480-3321journal_volume
38pub_type
杂志文章相关文献
GENOME文献大全abstract::The present study constitutes the first attempt to construct a photographic map of the polytene chromosomes of Dacus oleae, a pest of the olive tree that causes serious financial damage in all olive oil producing countries. The map was constructed by using the larval fat body cells, the chromosomes of which are repres...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g92-056
更新日期:1992-06-01 00:00:00
abstract::Canada wild rye (CWR, Elymus canadensis L., 2n = 4x = 28) is a potential source of genes for disease resistance and environmental tolerance in barley (Hordeum vulgare L., 2n = 2x = 14). Tissue cultures were initiated from immature inflorescences of CWR x 'Betzes' barley hybrids to promote CWR introgression into barley...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-047
更新日期:1996-04-01 00:00:00
abstract::MicroRNAs (miRNAs) repress target genes at the post-transcriptional level and play important roles in development and cell lineage decisions. In vertebrates, however, both the targets of miRNAs and their expression profiles during development are poorly understood. Thus far, 326 human miRNAs, 249 mouse miRNAs, and 195...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-120
更新日期:2006-10-01 00:00:00
abstract::Nematodes are frequently cited as underrepresented in faunistic surveys using DNA barcoding with COI. This underrepresentation is generally attributed to a limited presence of nematodes in DNA databases which, in turn, is often ascribed to structural variability and high evolutionary rates in nematode mitochondrial ge...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2019-0196
更新日期:2020-06-11 00:00:00
abstract::Twenty-nine lines with deletions in the Lr19 ('Indis') translocated chromosome segment were used to physically map three Thinopyrum RFLP loci as well as the Sr25 and Sd1 loci. From the data, the relative locations of marker loci on the translocation were determined as: Sd1, Xpsr165, Xpsr105, Xps129, Lr19, Wsp-D1, Sr25...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-126
更新日期:1996-10-01 00:00:00
abstract::The genomic origin of alien chromosomes present in a wheat-Thinopyrum intermedium partial amphiploid TAF46 (2n = 8x = 56) and six derived chromosome addition lines were analyzed by genomic in situ hybridization (GISH) using S genomic DNA from Pseudoroegneria strigosa (2n = 2x = 14, SS) as a probe. The GISH analysis cl...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g99-090
更新日期:1999-12-01 00:00:00
abstract::Whole-genome sequencing is fundamental to understanding the genetic composition of an organism. Given the size and complexity of the soybean genome, an alternative approach is targeted random-gene sequencing, which provides an immediate and productive method of gene discovery. In this study, more than 120000 soybean e...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-150
更新日期:2002-04-01 00:00:00
abstract::Among 15 wild perennial species of the genus Glycine Willd. subgenus Glycine, G. tomentella is exceptional. It is composed of four cytotypes (2n = 38, 40, 78, 80), is diverse in morphological features, and covers a wide geographical area. The objectives of this study were to uncover the genomic diversity in 78- and 80...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-063
更新日期:1994-06-01 00:00:00
abstract::A F2 population of two celery cultivated types (Apium graveolens L. var. rapaceum and A. graveolens L. var. secalinum) was used to construct a linkage map consisting of 29 RFLP (restriction fragment length polymorphism), 100 RAPD (random amplified polymorphic DNA), four isozyme, one disease resistance, and one growth ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-005
更新日期:1995-02-01 00:00:00
abstract::This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-117
更新日期:1996-10-01 00:00:00
abstract::Both meiosis-specific and general recombination functions, recruited from the mitotic cell cycle, are required for elevated levels of recombination and for chromosome synapsis (assembly of the synaptonemal complex) during yeast meiosis. The meiosis-specific SPO11 gene (previously shown to be required for meiotic recom...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g89-017
更新日期:1989-01-01 00:00:00
abstract::The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines wa...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::The fleshless berry (flb) mutation of grapevine (Vitis vinifera L. 'Ugni Blanc') impairs the differentiation and division of inner mesocarp cells responsible for flesh in grapevine berries. In order to study the inheritance of the mutation and to map the flb locus, 5 segregating populations were created. Progeny plant...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-034
更新日期:2006-07-01 00:00:00
abstract::The objectives of the present research were to determine the effects of water stress on seed-quality traits and to map QTLs controlling the studied traits under two different water treatments in a population of sunflower recombinant inbred lines (RILs). Two experiments were conducted in greenhouse and field conditions...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-038
更新日期:2008-08-01 00:00:00
abstract::As part of a project on lentil molecular genetics, the sequence of the 18S-25S ribosomal RNA gene intergenic spacer (IGS) of Lens culinaris Medik. was determined. DNA was cloned after polymerase chain reaction (PCR) amplification. The spacer of 2939 bp was composed of nonrepetitive sequences and four tandem arrays of ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-022
更新日期:2000-08-01 00:00:00
abstract::Doubled haploid (DH) lines together with a cotyledon bioassay were employed for the molecular analysis of resistance to the blackleg fungus Leptosphaeria maculans in the Australian Brassica napus cultivars Shiralee and Maluka. We used bulked segregant analysis to identify 13 RAPD and two RFLP markers linked to the res...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-041
更新日期:1997-06-01 00:00:00
abstract::Five nonallelic copies of the dispersed (GATA)n repeated sequence of Drosophila melanogaster (referred to as GATA elements) have been sequenced and analysed. The GATA elements range in size from 111 to 444 bp, consisting predominantly of tandemly repeated GATAs, interspersed with variants of the subunit. The types and...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g90-113
更新日期:1990-10-01 00:00:00
abstract::Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as g...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g92-014
更新日期:1992-02-01 00:00:00
abstract::Genetic diversity of 33 Elymus caninus accessions was investigated using isozyme, RAPD, and microsatellite markers. The three assays differed in the amount of polymorphism detected. Microsatellites detected the highest polymorphism. Six microsatellite primer pairs generated a total of 74 polymorphic bands (alleles), w...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::In multi-cellular organisms, failure to properly regulate cell-cycle progression can result in inappropriate cell death or uncontrolled cell division leading to tumor formation. To guard against such events, conserved regulatory mechanisms called "checkpoints" block progression into mitosis in response to DNA damage a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-051
更新日期:2002-10-01 00:00:00
abstract::Multiple reaction monitoring mass spectrometry (MRM-MS) is an emerging technology for blood biomarker verification and validation; however, the results may be influenced by pre-analytical factors. This exploratory study was designed to determine if differences in phlebotomy techniques would significantly affect the ab...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0036
更新日期:2015-12-01 00:00:00
abstract::Mitochondrial (mt) genome sequences of insects are receiving renewed attention in molecular phylogentic studies, studies of mt-genome rearrangement, and other unusual molecular phenomena, such as translational frameshifting. At present, the basal neopteran lineages are poorly represented by mt-genome sequences. Comple...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-037
更新日期:2006-07-01 00:00:00
abstract::Forty apricot cultivars with different geographic origins belonging to the germplasm collections of St. Istvan University (Budapest, Hungary) and the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were studied by means of SSR markers. The aim of the study was to determine the genetic relatio...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-128
更新日期:2003-04-01 00:00:00
abstract::Cashew (Anacardium occidentale L.), introduced into India about 400 years ago, is distributed widely in the coastal regions as spontaneous populations as well as in cultivation. Despite the plant's commercial exploitation, little is known about its actual introduction and diversification. We attempted to construct the...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-119
更新日期:2009-03-01 00:00:00
abstract::High-throughput sequencing (HTS) of DNA barcodes (metabarcoding), particularly when combined with standardized sampling protocols, is one of the most promising approaches for censusing overlooked cryptic invertebrate communities. We present biodiversity estimates based on sequencing of the cytochrome c oxidase subunit...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0208
更新日期:2016-09-01 00:00:00
abstract::Genomic in situ hybridization (GISH) is one of the most popular and effective techniques for detecting alien chromatin introgressed into breeding lines; however, GISH analysis alone does not reveal the genetic identity of the alien chromosomes. We previously isolated a set of bacterial artificial chromosomes (BACs) sp...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::In an initial investigation of differential expression of genes caused by cis-acting regulatory elements in rice, the lack of reproducibility led us to question the basic premise of allelic expression imbalance determination: namely that departures of cDNA expression ratios from those observed in genomic DNA provide u...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-023
更新日期:2012-05-01 00:00:00
abstract::Paenibacillus terrae NK3-4 is a plant growth-promoting rhizobacterium that may be useful for controlling plant diseases. We conducted a genomic analysis and identified the genes mediating antimicrobial functions. Additionally, an extracellular antifungal protein component was isolated and identified. The draft genome ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0113
更新日期:2018-10-01 00:00:00
abstract::We have used nuclear and chloroplast molecular markers to genotype cultivated and wild accessions of Vitis vinifera L. from Tunisia and assess their genetic relationships. Fifty-five distinct genotypes were identified among 80 cultivated accessions, including 18 genotypic groups containing between 2 and 5 accessions p...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-072
更新日期:2004-12-01 00:00:00
abstract::Using pseudomolecules of assembled genomic sequence, we computed the frequencies of 6 to 24 bp oligonucleotide (oligo) "words" across the genome of rice (Oryza sativa L. subsp. japonica). All oligos of 10 or fewer basepairs were repeated at least 12 times in the genome. The percentage of unique (non-repeated) oligos r...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-055
更新日期:2006-08-01 00:00:00