Abstract:
:Among 15 wild perennial species of the genus Glycine Willd. subgenus Glycine, G. tomentella is exceptional. It is composed of four cytotypes (2n = 38, 40, 78, 80), is diverse in morphological features, and covers a wide geographical area. The objectives of this study were to uncover the genomic diversity in 78- and 80-chromosome cytotypes through a multidisciplinary approach, using cytogenetic, biochemical, and molecular methods, to verify previously identified isozyme groupings and to determine their possible origins. The cytogenetic observations, total seed protein and protease inhibitor profile comparisons, and the phylogenetic analysis of restriction fragment length polymorphisms identified three distinct groups (T1, T5, T6) among aneutetraploid (2n = 78) and four distinct groups (T2, T3, T4, T7) among tetraploid (2n = 80) G. tomentella accessions. The groupings were congruent with those of isozyme analysis. Tetraploid accessions from Indonesia were assigned to a new group, T7, based on the present study. Morphology, cytology, and seed protein banding patterns of synthetic tetraploids indicated that the T1 and T5 group aneutetraploids were composed of D3D3EE and AAEE genomes, respectively, and the T2 group tetraploid accessions consisted of AAD3D3 genomes. Various groups within the 78- and 80-chromosome G. tomentella were suggested to have originated in Australia by allopolyploidization, most likely through multiple independent events.
journal_name
Genomejournal_title
Genomeauthors
Kollipara KP,Singh RJ,Hymowitz Tdoi
10.1139/g94-063subject
Has Abstractpub_date
1994-06-01 00:00:00pages
448-59issue
3eissn
0831-2796issn
1480-3321pii
g94-063journal_volume
37pub_type
杂志文章相关文献
GENOME文献大全abstract::Comparative mapping is an important component of map-based cloning in large-genome cereal species. We describe evidence of a segmental chromosomal duplication harbouring CONSTANS-like genes in barley that predates the divergence of the Oryzoideae (rice) and Pooideae (brachypodium, barley, wheat) clades, and discuss th...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-101
更新日期:2010-03-01 00:00:00
abstract::The "rings" belonging to the genus Ypthima are amongst the most common butterflies in Peninsular Malaysia. However, the species can be difficult to tell apart, with keys relying on minor and often non-discrete ring characters found on the hindwing. Seven species have been reported from Peninsular Malaysia, but this is...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0156
更新日期:2016-10-01 00:00:00
abstract::We have developed 21 new microsatellites in the model diploid perennial species Fragaria vesca from an enriched genomic library developed using F. vesca 'Ruegen'. The transferability of the primer pairs to other Fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawber...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-142
更新日期:2004-06-01 00:00:00
abstract::Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g02-049
更新日期:2002-10-01 00:00:00
abstract::Rhubarb (official Da-huang) is an important medicinal herb in Asia. Many adulterants of official Da-huang have been discovered in Chinese markets in recent years, which has resulted in adverse effects in medicinal treatment. Here, novel molecular markers based on a short maturase K (matK) gene were developed for authe...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2012-0182
更新日期:2013-02-01 00:00:00
abstract::Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. In the present study, 3 cDNA libraries generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genom...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-007
更新日期:2007-03-01 00:00:00
abstract::Ginkgo biloba L. (known as ginkgo or maidenhair tree) is a phylogenetically isolated, charismatic, gymnosperm tree. Herbal dietary supplements, prepared from G. biloba leaves, are consumed to boost cognitive capacity via improved blood perfusion and mitochondrial function. A novel DNA mini-barcode assay was designed a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2014-0130
更新日期:2014-09-01 00:00:00
abstract::In order to map the genes conditioning the induction of embryos during our anther culture process, we evaluated F2 plants from three different crosses for their anther culture ability and also performed RFLP analysis on these plants. The results showed that six chromosomal regions appear to be associated with the abil...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-127
更新日期:1995-10-01 00:00:00
abstract::Most information about the molecular biology of phaseolin, the major seed storage protein in Phaseolus vulgaris, has been obtained from the T-type phaseolin, which is characteristic of the Andean gene pool of the species. In the work reported here, two cDNA clones for the S-type phaseolin representing the other major,...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-107
更新日期:1994-10-01 00:00:00
abstract::The 2C and 4C nuclear DNA amounts were estimated in eight diploid species, belonging to three diverse genera (Vicia, Tephrosia, and Phlox) and their corresponding colchitetraploids. In P. drummondii, T. purpurea, and T. oxygona tetraploids the deviation from the expectation was highly significant. The DNA in P. drummo...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-080
更新日期:1994-08-01 00:00:00
abstract::Restriction endonuclease TaqI has been known as a nonbanding restriction endonuclease when it is used on fixed human chromosomes. However, a specific TaqI digestion can be obtained after varying experimental conditions such as concentration of enzyme, time of incubation, and volume of the final reaction mixture. This ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g91-039
更新日期:1991-04-01 00:00:00
abstract::Cla elements are a family of tandem repetitive DNA sequences present in the genome of several Chironomus species. Interspersed clusters of Cla elements are widely distributed all over the chromosomes in C. thummi thummi, while they seem to be limited to the centromeric regions in the closely related subspecies C. t. p...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-077
更新日期:1994-08-01 00:00:00
abstract::5S rDNA loci have been mapped on barley chromosomes by in situ hybridization using five reciprocal translocation lines. Two kinds of DNA probes covering either the 5S rDNA coding region or the 5S rDNA coding and flanking noncoding regions were used. They were prepared by direct cloning from interphase nuclei and simul...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g94-013
更新日期:1994-02-01 00:00:00
abstract::A novel tandemly repeated DNA structure of Toxoplasma gondii that meets the requirements assigned for satellital DNA was characterized. A DNA fragment of 1002 bp contains two different elements of repetitive DNA families named ABGTg7 and ABGTg8.2. Both repeats are members of a more complex tandem structure where ABGTg...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g98-120
更新日期:1999-04-01 00:00:00
abstract::Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridizati...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-084
更新日期:2000-12-01 00:00:00
abstract::The Himalayan marmot (Marmota himalayana), a natural host and transmitter of plague, is also susceptible to the hepadnavirus infection. To reveal the genetic basis of the hepadnavirus susceptibility and the immune response to plague, we systematically characterized the features of immune genes in Himalayan marmot with...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2019-0161
更新日期:2020-06-01 00:00:00
abstract::Fusarium head blight (FHB) is one of the most important fungal wheat diseases worldwide. Understanding the genetics of FHB resistance is the key to facilitating the introgression of different FHB resistance genes into adapted wheat. The objectives of the present study were to detect and map quantitative trait loci (QT...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-104
更新日期:2005-04-01 00:00:00
abstract::Despite the recent advances in plant population genetic studies, the lack of information regarding pedigree, ploidy level, or mode of inheritance for many polyploids can compromise the analysis of the molecular data produced. The aim of this study was to examine both microsatellite and cytogenetic characteristics of t...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2013-0159
更新日期:2013-12-01 00:00:00
abstract::Conceptual aspects of estimation of genetic components of variance and covariance under selection are discussed, with special attention to likelihood methods. Certain selection processes are described and alternative likelihoods that can be used for analysis are specified. There is a mathematical relationship between ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g89-136
更新日期:1989-01-01 00:00:00
abstract::Lycopersicon esculentum (tomato) has a small genome (2C = 1.90 pg of DNA) packaged in 2n = 2x = 24 small acrocentric to metacentric chromosomes. Like the chromosomes of other members of the family Solanaceae, tomato chromosomes have pericentromeric heterochromatin. To determine the fraction of the tomato genome found ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-011
更新日期:1996-02-01 00:00:00
abstract::Cashew (Anacardium occidentale L.), introduced into India about 400 years ago, is distributed widely in the coastal regions as spontaneous populations as well as in cultivation. Despite the plant's commercial exploitation, little is known about its actual introduction and diversification. We attempted to construct the...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G08-119
更新日期:2009-03-01 00:00:00
abstract::The morphological picture of primate phylogeny has not unambiguously identified the nearest outgroup of Anthropoidea and has not resolved the branching pattern within Hominoidea. The molecular picture provides more resolution and clarifies the systematics of Hominoidea. Protein and DNA evidence divides Hominoidea into...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/g89-050
更新日期:1989-01-01 00:00:00
abstract::Despite being a controversial crop, Cannabis sativa L. has a long history of cultivation throughout the world. Following recent legalisation in Canada, it is emerging as an important plant for both medicinal and recreational purposes. Recent progress in genome sequencing of both cannabis and hemp varieties allows for ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0087
更新日期:2020-11-13 00:00:00
abstract::We sequenced the entire coding region of the mitochondrial genome of Heterodera glycines. The sequence obtained comprised 14.9 kb, with PCR evidence indicating that the entire genome comprised a single, circular molecule of approximately 21-22 kb. The genome is the most T-rich nematode mitochondrial genome reported to...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g11-024
更新日期:2011-07-01 00:00:00
abstract::We are building a framework physical infrastructure across the soybean genome by using SSR (simple sequence repeat) and RFLP (restriction fragment length polymorphism) markers to identify BACs (bacterial artificial chromosomes) from two soybean BAC libraries. The libraries were prepared from two genotypes, each digest...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::In this study we sequenced and analysed the complete mitochondrial (mt) genome of the Chilean predatory mite Phytoseiulus persimilis Athias-Henriot (Chelicerata: Acari: Mesostigmata: Phytoseiidae: Amblyseiinae). The 16 199 bp genome (79.8% AT) contains the standard set of 13 protein-coding and 24 RNA genes. Compared w...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-004
更新日期:2010-04-01 00:00:00
abstract::We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment le...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::We have used immunocytochemistry and molecular cloning methods to identify and characterize structural polypeptides of the centromere. These studies permit us to resolve two distinct regions: the inner and outer centromere. (i) Components of the outer centromere: autoantibodies from certain patients with rheumatic dis...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/g89-103
更新日期:1989-01-01 00:00:00
abstract::Environmental DNA (eDNA) is emerging as a potentially powerful tool for inferring species' presence, and hence occupancy, from DNA that is shed into environmental samples such as water. Although eDNA screening has been used to detect DNA from a variety of taxonomic groups, it has not yet been used to identify DNA from...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0196
更新日期:2016-12-01 00:00:00
abstract::Visual quality of field pea (Pisum sativum L.) is one of the most important determinants of the market value of the harvested crop. Seed coat color, seed shape, and seed dimpling are the major components of visual seed quality of field pea and are considered as important breeding objectives. The objectives of this res...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g10-117
更新日期:2011-04-01 00:00:00