Abstract:
:In an initial investigation of differential expression of genes caused by cis-acting regulatory elements in rice, the lack of reproducibility led us to question the basic premise of allelic expression imbalance determination: namely that departures of cDNA expression ratios from those observed in genomic DNA provide unequivocal evidence of cis-acting polymorphisms. This paper describes experiments designed to demonstrate that stochastic variation in low copy number of targets in PCR reactions give variable allelic ratios even when starting with the same copy numbers of the two alleles. These significant departures from an expected 1:1 ratio provide an explanation to the lack of reproducibility observed for our cDNA measurements.
journal_name
Genomejournal_title
Genomeauthors
Akkinepalli H,Ereful N,Liu Y,Malabanan K,Howells R,Stamati K,Powell W,Leung H,Greenland A,Mackay I,Lee Ddoi
10.1139/g2012-023subject
Has Abstractpub_date
2012-05-01 00:00:00pages
400-6issue
5eissn
0831-2796issn
1480-3321journal_volume
55pub_type
杂志文章相关文献
GENOME文献大全abstract::We sequenced the entire coding region of the mitochondrial genome of Heterodera glycines. The sequence obtained comprised 14.9 kb, with PCR evidence indicating that the entire genome comprised a single, circular molecule of approximately 21-22 kb. The genome is the most T-rich nematode mitochondrial genome reported to...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g11-024
更新日期:2011-07-01 00:00:00
abstract::Striga gesnerioides is a root hemiparasite that primarily parasitizes dicotyledonous species, including cowpea (Vigna unguiculata L.) and other legumes. Based on the differential resistance response of various cultivars, landraces, and breeding lines, it has been proposed that several distinct races of cowpea-parasiti...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-086
更新日期:2006-11-01 00:00:00
abstract::Insulin-like growth factor 1 (IGF1) regulates growth and metabolic functions in vertebrates. A dinucleotide repeat sequence located at the promoter region of the IGF1 gene has been reported in several vertebrate species and may affect the control of the transcriptional activity of this gene. The genotypes of animals f...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Segregation patterns of polymorphic simple sequence repeat (SSR) primer pairs were investigated in monoploid potato families derived from anther culture. A total of 14 primers developed from the sequences in the database, as well as from a genomic library of potato, was used. Distorted segregation was observed for sev...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-140
更新日期:2002-04-01 00:00:00
abstract::The emergence of next generation sequencing has increased by several orders of magnitude the amount of data available for phylogenetics. Reduced representation approaches, such as restriction-sited associated DNA sequencing (RADseq), have proven useful for phylogenetic studies of non-model species at a wide range of p...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0202
更新日期:2017-09-01 00:00:00
abstract::TBP (tubulin-based polymorphism) is a new molecular marker based tool that relies on the presence of intron-specific DNA polymorphisms of the plant beta-tubulin gene family. The multifunctional and essential role of the tubulin proteins is reflected in the conservation of regions within their primary amino acid sequen...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-132
更新日期:2004-04-01 00:00:00
abstract::Gene segregation at six protein loci was analysed in progeny from tetraploid males and females obtained by suppression of first mitosis. The triploid full-sib families from five tetraploid males and the diploid gynogenetic lines from four tetraploid females were examined. The proportions of heterozygous gametes (0.83 ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g88-092
更新日期:1988-08-01 00:00:00
abstract::Microsatellites have many desirable marker properties and have been increasingly used in crop plants in genetic diversity studies. Here we report on the characterisation of microsatellite markers and on their use for the determination of genetic identities and the assessment of genetic variability among accessions fro...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-44-5-773
更新日期:2001-10-01 00:00:00
abstract::This paper describes a series of winter wheat - winter barley disomic addition lines developed from hybrids between winter wheat line Triticum aestivum L. 'Martonvásári 9 kr1' and the German 2-rowed winter barley cultivar Hordeum vulgare L. 'Igri'. The barley chromosomes in a wheat background were identified from the ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g06-134
更新日期:2007-01-01 00:00:00
abstract::High-throughput sequencing (HTS) of DNA barcodes (metabarcoding), particularly when combined with standardized sampling protocols, is one of the most promising approaches for censusing overlooked cryptic invertebrate communities. We present biodiversity estimates based on sequencing of the cytochrome c oxidase subunit...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0208
更新日期:2016-09-01 00:00:00
abstract::Carrot (Daucus carota L.) chromosomes are small and poorly differentiated in size and morphology. Here we demonstrate that fluorescent in situ hybridization (FISH) signals derived from arbitrary PCR probes can be used for chromosome identification in carrot. To prepare probes, we searched for nonpolymorphic products a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-003
更新日期:2012-03-01 00:00:00
abstract::Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with 4 genotypes of known Vrn genes, using previ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-028
更新日期:2007-05-01 00:00:00
abstract::Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obta...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-077
更新日期:1997-10-01 00:00:00
abstract::The genetic control of adult plant resistance to Stagonospora nodorum blotch (SNB) is complex, consisting of genes with minor effects interacting in an additive manner. Earlier studies detected quantitative trait loci (QTL) for flag leaf resistance in successive years on chromosomes 1B, 2A, 2D, and 5B using SSR- and D...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0203
更新日期:2018-02-01 00:00:00
abstract::Light-harvesting-like (LIL) proteins are low-molecular-mass membrane proteins related to the light-harvesting complexes, which form the dominant antenna system in most photosynthetic eukaryotes. To analyze the LIL protein family, we mined a number of publicly available databases to identify members of this family in a...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g09-081
更新日期:2010-01-01 00:00:00
abstract::We are building a framework physical infrastructure across the soybean genome by using SSR (simple sequence repeat) and RFLP (restriction fragment length polymorphism) markers to identify BACs (bacterial artificial chromosomes) from two soybean BAC libraries. The libraries were prepared from two genotypes, each digest...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Recent genome size estimates for Arctic amphipods have revealed the largest genomes known in the Crustacea. Here we provide additional data for 7 species of caridean shrimp collected from the Canadian Arctic and the Gulf of St. Lawrence. Genome sizes were estimated by flow cytometry and haploid C-values ranged from 8....
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-108
更新日期:2008-02-01 00:00:00
abstract::Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridizati...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-084
更新日期:2000-12-01 00:00:00
abstract::The European cultivated potato, Solanum tuberosum subsp. tuberosum, has 6 related cultivated species and more than 200 wild relatives. In Solanum spp., studies of cytoplasmic organelles have been mainly confined to the plastid DNA composition of cultivated and wild species. In this study, 53 genotypes of 30 potato spe...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-042
更新日期:2007-08-01 00:00:00
abstract::Our goal was to purify and characterize the allelic variants H1b1 and H1b2 of histone H1.b, one of the seven subtypes of this linker histone extracted from Japanese quail erythrocyte nuclei. These variants are revealed phenotypically as band H1.3 or part of band H1.4 by polyacrylamide gel electrophoresis (PAGE) in sod...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::Despite being a controversial crop, Cannabis sativa L. has a long history of cultivation throughout the world. Following recent legalisation in Canada, it is emerging as an important plant for both medicinal and recreational purposes. Recent progress in genome sequencing of both cannabis and hemp varieties allows for ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2020-0087
更新日期:2020-11-13 00:00:00
abstract::We have developed 21 new microsatellites in the model diploid perennial species Fragaria vesca from an enriched genomic library developed using F. vesca 'Ruegen'. The transferability of the primer pairs to other Fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawber...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-142
更新日期:2004-06-01 00:00:00
abstract::Being an essential mineral nutrient, potassium (K+) plays numerous important roles in plant growth and development and determines the yield and quality of crop products. The cellular level of K+ is controlled to a large extent by the K+ transporter, which belongs to the KT/HAK/KUP (HAK) family. However, little is know...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2018-0187
更新日期:2019-04-01 00:00:00
abstract::Bruchids (Coleoptera: Bruchidae) can cause serious damage to mungbean and several other leguminous crops and there is a strong association between small seed size and bruchid resistance. In investigating the feasibility of breeding large-seeded cultivars with high levels of bruchid resistance, we studied the relations...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/G09-031
更新日期:2009-07-01 00:00:00
abstract::Amplified fragment length polymorphism (AFLP) was used to obtain information on the within-species genetic variability of the tobacco cyst-nematode (TCN) complex. AFLP was found to be well suited to this type of study. The current classification of TCN was confirmed. Results indicate that the Globodera tabacum solanac...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-091
更新日期:2001-12-01 00:00:00
abstract::Phytophthora palmivora causes pod rot, a serious disease on cocoa widespread throughout the producing regions. In order to ascertain the genetic determination of cocoa resistance to P. palmivora, a study was carried out on two progenies derived from crosses between a heterozygous, moderately resistant Forastero clone,...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-44-1-79
更新日期:2001-02-01 00:00:00
abstract::Using a complementary (c)DNA-amplified fragment length polymorphism (AFLP) approach, we investigated differential gene expression linked to resistance mechanisms during the incompatible potato - Globodera pallida interaction. Expression was compared between a resistant and a susceptible potato clone, inoculated or not...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-015
更新日期:2007-04-01 00:00:00
abstract::In the silkworm Bombyx mori, non-susceptibility to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is located on chromosome 15. Owing to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progeny were used for...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g05-125
更新日期:2006-04-01 00:00:00
abstract::Numerous quantitative trait loci (QTL) have been detected in Brassica species, but fine-mapping of major QTL has advanced slowly. The development of functional markers can overcome this barrier. We used publicly available PlantGDB-assembled unique transcripts (PUTs) from Brassica species to design 7836 functional simp...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g11-042
更新日期:2011-09-01 00:00:00
abstract::The standard karyotype and a wide array of repatterned cytotypes from 21 demes of the double-allotriploid thelytokous Bacillus lynceorum have been analyzed by means of Giemsa, C-banding, and silver-staining techniques. The present study substantially amends the first karyotype description and also analyzes in detail t...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-044
更新日期:1993-04-01 00:00:00