A method for assaying the sensitivity of Drosophila replication checkpoint mutants to anti-cancer and DNA-damaging drugs.

abstract：:Carrot (Daucus carota L.) chromosomes are small and poorly differentiated in size and morphology. Here we demonstrate that fluorescent in situ hybridization (FISH) signals derived from arbitrary PCR probes can be used for chromosome identification in carrot. To prepare probes, we searched for nonpolymorphic products a...

journal_title：Genome

authors： Nowicka A,Grzebelus E,Grzebelus D

更新日期：2012-03-01 00:00:00

abstract：:The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) x Nigerian Local (NL) and ZHOU (a hull-less type) x WB. The integrated C. moschata map comprises 205 SSR markers and two morph...

journal_title：Genome

authors： Gong L,Pachner M,Kalai K,Lelley T

更新日期：2008-11-01 00:00:00

abstract：:Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obta...

journal_title：Genome

authors： Pedersen C,Langridge P

更新日期：1997-10-01 00:00:00

abstract：:Striga gesnerioides is a root hemiparasite that primarily parasitizes dicotyledonous species, including cowpea (Vigna unguiculata L.) and other legumes. Based on the differential resistance response of various cultivars, landraces, and breeding lines, it has been proposed that several distinct races of cowpea-parasiti...

journal_title：Genome

authors： Botanga CJ,Timko MP

更新日期：2006-11-01 00:00:00

abstract：:The RFLP locus Xglk605 identified by the probe Tag605 maps to a proximal position on the long arm of wheat chromosome 2B about 7 cM away from a gene conditioning resistance to cereal cyst nematode in the wheat line AUS10894. The clone Tag605 was partially sequenced and the PCR primer set AWP1 was designed. The 292-bp ...

journal_title：Genome

authors： Williams KJ,Fisher JM,Langridge P

更新日期：1996-08-01 00:00:00

abstract：:Vast differences exist in genome sizes of higher plants; however, gene count remains relatively constant among species. Differences observed in DNA content can be attributed to retroelement amplification leading to genome expansion. Cytological and genetic studies have demonstrated that genes are clustered in islands ...

journal_title：Genome

authors： Brooks SA,Huang L,Gill BS,Fellers JP

更新日期：2002-10-01 00:00:00

abstract：:Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construc...

journal_title：Genome

authors： Ahmad NS,Redjeki ES,Ho WK,Aliyu S,Mayes K,Massawe F,Kilian A,Mayes S

更新日期：2016-07-01 00:00:00

abstract：:Canada wild rye (CWR, Elymus canadensis L., 2n = 4x = 28) is a potential source of genes for disease resistance and environmental tolerance in barley (Hordeum vulgare L., 2n = 2x = 14). Tissue cultures were initiated from immature inflorescences of CWR x 'Betzes' barley hybrids to promote CWR introgression into barley...

journal_title：Genome

authors： Dahleen LS

更新日期：1996-04-01 00:00:00

abstract：:The molecular characterization of C-banded regions of Secale montanum Guss. by means of in situ hybridization was performed in order to provide new information about their chromosome structure relative to cultivated rye, Secale cereale L. Accurate identification of individual chromosomes was achieved using simultaneou...

journal_title：Genome

authors： Cuadrado A,Jouve N

更新日期：1995-08-01 00:00:00

abstract：:The nearly complete mitochondrial (mt) genome of an egg parasitoid, Trissolcus basalis (Wollaston), was sequenced using both 454 and Illumina next-generation sequencing technologies. A portion of the noncoding region remained unsequenced, possibly owing to the presence of repeats. The sequenced portion of the genome i...

journal_title：Genome

authors： Mao M,Valerio A,Austin AD,Dowton M,Johnson NF

更新日期：2012-03-01 00:00:00

abstract：:Pigeon spermatocytes were processed with a drying-down technique and their synaptonemal complex (SC) complements were analyzed by electron microscopy. The synaptonemal complex karyotype of the macrobivalents shows an excellent correspondence with the mitotic karyotype. The number and distribution of recombination nodu...

journal_title：Genome

authors： Pigozzi MI,Solari AJ

更新日期：1999-04-01 00:00:00

abstract：:Large sequence insertion (LSI) is one of the structural variations (SVs) that may cause phenotypic differences in plants. To identify the LSIs using medium-coverage sequencing data of four wild soybean (Glycine soja) genotypes, we designed a hybrid approach combining de novo assembly and read mapping. Total reads and ...

journal_title：Genome

authors： Lee T,Kim MY,Ha J,Lee SH

更新日期：2018-10-01 00:00:00

abstract：:Hordeum vulgare subsp. spontaneum, the wild progenitor of barley, is a potential source of useful genetic variation for barley breeding programs. The objective of this study was to map quantitative trait loci (QTLs) in an advanced backcross population of barley. A total of 207 BC3 lines were developed using the 2-rowe...

journal_title：Genome

authors： Li JZ,Huang XQ,Heinrichs F,Ganal MW,Röder MS

更新日期：2006-05-01 00:00:00

abstract：:Cuscuta is a widely distributed genus of holoparasitic plants. Holocentric chromosomes have been reported only in species of one of its subgenera (Cuscuta subg. Cuscuta). In this work, a representative of this subgenus, Cuscuta approximata, was investigated looking for its mitotic and meiotic chromosome behaviour and ...

journal_title：Genome

authors： Guerra M,García MA

更新日期：2004-02-01 00:00:00

abstract：:Genomic in situ hybridization (GISH) is one of the most popular and effective techniques for detecting alien chromatin introgressed into breeding lines; however, GISH analysis alone does not reveal the genetic identity of the alien chromosomes. We previously isolated a set of bacterial artificial chromosomes (BACs) sp...

journal_title：Genome

authors： Dong F,McGrath JM,Helgeson JP,Jiang J

更新日期：2001-08-01 00:00:00

abstract：:Oilseed rape (Brassica napus) is an allotetraploid species consisting of two genomes, derived from B. rapa (A genome) and B. oleracea (C genome). The presence of these two genomes makes single nucleotide polymorphism (SNP) marker identification and SNP analysis more challenging than in diploid species, as for a given ...

journal_title：Genome

authors： Durstewitz G,Polley A,Plieske J,Luerssen H,Graner EM,Wieseke R,Ganal MW

更新日期：2010-11-01 00:00:00

abstract：:The first quantitative trait locus (QTL) analysis of multiple agronomic traits in the model legume Lotus japonicus was performed with a population of recombinant inbred lines derived from Miyakojima MG-20 x Gifu B-129. Thirteen agronomic traits were evaluated in 2004 and 2005: traits of vegetative parts (plant height,...

journal_title：Genome

authors： Gondo T,Sato S,Okumura K,Tabata S,Akashi R,Isobe S

更新日期：2007-07-01 00:00:00

abstract：:The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identifie...

journal_title：Genome

authors： Heng HH,Liu G,Lu W,Bremer S,Ye CJ,Hughes M,Moens P

更新日期：2001-04-01 00:00:00

abstract：:Somatic karyotypes of the nine annual species of Cicer (2n = 16) were analyzed using C-banding. Highly significant differences in haploid genome length and C-band positive heterochromatin content were observed. The haploid genome length ranged from 20.0 μm in the wild species C. judaicum to 28.7 μm in the cultiv...

journal_title：Genome

authors： Tayyar RI,Lukaszewski AJ,Waines JG

更新日期：1994-08-01 00:00:00

abstract：:The superoxide dismutases (SODs) protect oxygen-using cells against reactive oxygen species, the potentially toxic by-products of respiration, oxidative metabolism, and radiation. We have previously shown that genetic disruption of CuZn SOD (SOD1) in Drosophila imparts a recessive phenotype of reduced lifespan, infert...

journal_title：Genome

authors： Woodruff RC,Phillips JP,Hilliker AJ

更新日期：2004-12-01 00:00:00

abstract：:Intergeneric hybridizations have been made between species of Lolium and Festuca. It has been demonstrated, largely through conventional cytogenetic analysis, that the genomes of the two genera are related, however, much information is lacking on exactly how closely related the genomes are between the two species. We ...

journal_title：Genome

authors： Cao M,Sleper DA,Dong F,Jiang J

更新日期：2000-04-01 00:00:00

abstract：:The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capit...

journal_title：Genome

authors： Zhao JT,Frommer M,Sved JA,Zacharopoulou A

更新日期：1998-08-01 00:00:00

abstract：:A high-density BAC filter of Triticum monococcum was screened for the presence of a centromeric retrotransposon using the integrase region as a probe. Southern hybridization to the BAC digests using total genomic DNA probes of Triticum monococcum, Triticum aestivum, and Hordeum vulgare detected differentially hybridiz...

journal_title：Genome

authors： Ito H,Nasuda S,Endo TR

更新日期：2004-08-01 00:00:00

abstract：:We report the characterization of 3 new repetitive sequences from the bivalve mollusc Mytilus galloprovincialis, designated Mg1, Mg2, and Mg3, with monomer lengths of 169, 260, and 70 bp, respectively. The 3 repeats together constitute approximately 7.8% of the M. galloprovincialis genome and were found, together with...

journal_title：Genome

authors： Kourtidis A,Drosopoulou E,Pantzartzi CN,Chintiroglou CC,Scouras ZG

更新日期：2006-11-01 00:00:00

abstract：:Configurations of mitochondrial coxI and orfB gene regions were analysed by polymerase chain reaction (PCR) in three wild and one cultivated species of Raphanus. A total of 207 individual plants from 60 accessions were used. PCR with five combinations of primers identified five different amplification patterns both in...

journal_title：Genome

authors： Yamagishi H,Terachi T

更新日期：2003-02-01 00:00:00

abstract：:The aim of this study was to investigate the differential expression characteristics and the roles of the genome-wide microRNAs (miRNAs) in immunoglobulin A nephropathy (IgAN) kidney tissues. We used Illumina high-throughput sequencing technology to evaluate the miRNAs expression of six biopsy tissues from IgAN and si...

journal_title：Genome

authors： Tan K,Chen J,Li W,Chen Y,Sui W,Zhang Y,Dai Y

更新日期：2013-03-01 00:00:00

abstract：:Being an essential mineral nutrient, potassium (K+) plays numerous important roles in plant growth and development and determines the yield and quality of crop products. The cellular level of K+ is controlled to a large extent by the K+ transporter, which belongs to the KT/HAK/KUP (HAK) family. However, little is know...

journal_title：Genome

authors： Song Z,Wu X,Gao Y,Cui X,Jiao F,Chen X,Li Y

更新日期：2019-04-01 00:00:00

abstract：:The evolution of Drosophila subobscura mitochondrial DNA has been studied in experimental populations, founded with flies from a natural population from Calvià (Majorca, Balearic Islands, Spain). This population, like others founded in Europe, is characterized by the presence of 2 very common (>95%) mitochondrial hapl...

journal_title：Genome

authors： Oliver P,Balanyà J,Ramon MM,Picornell A,Serra L,Moya A,Castro JA

更新日期：2005-12-01 00:00:00

abstract：:The octamer-binding transcription factor 4 gene (Oct4) plays a critical role in maintaining pluripotency during early mammalian embryonic development and self-renewal of embryonic stem (ES) cells. In this study, we cloned the Oct4 cDNA and 2.8-kb regulatory region upstream of the start codon in Guanzhong dairy goat ( ...

journal_title：Genome

authors： Cheng X,Meng S,Deng J,Lai W,Wang H

更新日期：2011-10-01 00:00:00

abstract：:Whole-genome sequencing is fundamental to understanding the genetic composition of an organism. Given the size and complexity of the soybean genome, an alternative approach is targeted random-gene sequencing, which provides an immediate and productive method of gene discovery. In this study, more than 120000 soybean e...

journal_title：Genome

authors： Shoemaker R,Keim P,Vodkin L,Retzel E,Clifton SW,Waterston R,Smoller D,Coryell V,Khanna A,Erpelding J,Gai X,Brendel V,Raph-Schmidt C,Shoop EG,Vielweber CJ,Schmatz M,Pape D,Bowers Y,Theising B,Martin J,Dante M,Wyl

更新日期：2002-04-01 00:00:00