SSR-based genetic linkage map of Cucurbita moschata and its synteny with Cucurbita pepo.

Abstract:

:The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) x Nigerian Local (NL) and ZHOU (a hull-less type) x WB. The integrated C. moschata map comprises 205 SSR markers and two morphological traits (Gr and n). The map is composed of 27 linkage groups with a marker density of 7 cM. Comparing the C. moschata map with the published Cucurbita pepo map, we found a high level of macrosynteny. Seventy-two of 76 common SSR markers between C. moschata and C. pepo were located in homologous linkage groups. These markers in general have conserved orders and similar genetic distances; they represent orthologous loci. A reference map based on these SSRs was obtained. No major chromosomal rearrangement between the two species could be detected at present, although four SSR markers were mapped in nonhomologous linkage groups. The comparative alignment of SSR markers did not provide any indication of a possible ancient polyploid origin of the species. The comparative mapping of C. moschata and C. pepo reported here will be useful for further studies on Cucurbit evolution, gene isolation, and breeding work.

journal_name

Genome

journal_title

Genome

authors

Gong L,Pachner M,Kalai K,Lelley T

doi

10.1139/G08-072

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

878-87

issue

11

eissn

0831-2796

issn

1480-3321

pii

g08-072

journal_volume

51

pub_type

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