C-banding plus fluorochrome staining shows differences in C-, G-, and R-bands in human and mouse metaphase chromosomes.

Abstract:

:C-banded slides stained with DAPI or chromomycin A3 show different banding patterns between human and L929 mouse cell line metaphase chromosomes, which are also different from those obtained with standard Giemsa C-banding or fluorochrome staining. Human metaphase chromosomes pretreated for C-banding and stained with DAPI show simultaneous C- and DA-DAPI banding patterns, whilst the mouse metaphase chromosomes show both C-banding and G/Q banding like patterns. However, the chromomycin A3 staining of pre-C-banded metaphase chromosomes reveals conspicuous R-banding in man that is absent in mouse. Chromatin species-specific structural factors would explain these results, which prevent simple comparisons of R-, G-, and C-bands among different organisms. The markers induced by this technique may be of practical use for chromosome identification in human-mouse somatic cell hybridization cultures.

journal_name

Genome

journal_title

Genome

authors

Bella JL,Fernández JL,Gosálvez J

doi

10.1139/g95-114

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

864-8

issue

5

eissn

0831-2796

issn

1480-3321

journal_volume

38

pub_type

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