Abstract:
:C-banded slides stained with DAPI or chromomycin A3 show different banding patterns between human and L929 mouse cell line metaphase chromosomes, which are also different from those obtained with standard Giemsa C-banding or fluorochrome staining. Human metaphase chromosomes pretreated for C-banding and stained with DAPI show simultaneous C- and DA-DAPI banding patterns, whilst the mouse metaphase chromosomes show both C-banding and G/Q banding like patterns. However, the chromomycin A3 staining of pre-C-banded metaphase chromosomes reveals conspicuous R-banding in man that is absent in mouse. Chromatin species-specific structural factors would explain these results, which prevent simple comparisons of R-, G-, and C-bands among different organisms. The markers induced by this technique may be of practical use for chromosome identification in human-mouse somatic cell hybridization cultures.
journal_name
Genomejournal_title
Genomeauthors
Bella JL,Fernández JL,Gosálvez Jdoi
10.1139/g95-114subject
Has Abstractpub_date
1995-10-01 00:00:00pages
864-8issue
5eissn
0831-2796issn
1480-3321journal_volume
38pub_type
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