Abstract:
:A genetic linkage map of Brassica rapa L. was constructed using recombinant inbred lines (RILs) derived from a cross between yellow-seeded cultivar Sampad and a yellowish brown seeded inbred line 3-0026.027. The RILs were evaluated for seed color under three conditions: field plot, greenhouse, and controlled growth chambers. Variation for seed color in the RILs ranged from yellow, like yellow sarson, to dark brown/black even though neither parent had shown brown/black colored seeds. One major QTL (SCA9-2) and one minor QTL (SCA9-1) on linkage group (LG) A9 and two minor QTL (SCA3-1, SCA5-1) on LG A3 and LG A5, respectively, were detected. These collectively explained about 67% of the total phenotypic variance. SCA9-2 mapped in the middle of LG A9, explained about 55% phenotypic variance, and consistently expressed in all environments. The second QTL on LG A9 was ~70 cM away from SCA9-2, suggesting that independent assortment of these QTLs is possible. A digenic epistatic interaction was found between the two main effect QTL on LG A9; and the epistasis × environment interaction was nonsignificant, suggesting stability of the interaction across the environments. The QTL effect on LG A9 was validated using simple sequence repeat (SSR) markers from the two QTL regions of this LG on a B(1)S(1) population (F(1) backcrossed to Sampad followed by self-pollination) segregating for brown and yellow seed color, and on their self-pollinated progenies (B(1)S(2)). The SSR markers from the QTL region SCA9-2 showed a stronger linkage association with seed color as compared with the marker from SCA9-1. This suggests that the QTL SCA9-2 is the major determinant of seed color in the A genome of B. rapa.
journal_name
Genomejournal_title
Genomeauthors
Kebede B,Cheema K,Greenshields DL,Li C,Selvaraj G,Rahman Hdoi
10.1139/g2012-066subject
Has Abstractpub_date
2012-12-01 00:00:00pages
813-23issue
12eissn
0831-2796issn
1480-3321journal_volume
55pub_type
杂志文章相关文献
GENOME文献大全abstract::Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) possesses a number of valuable genes against biotic and abiotic stress, which could be transferred into common wheat background for wheat improvement. In the present study, we determined the karyotypic constitution of a wheat - L. mollis double disomic addition line...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0151
更新日期:2017-05-01 00:00:00
abstract::The use of complementary RNA sequences such as antisense RNAs and ribozymes to regulate the expression of specific genes in eukaryotic cells has been well-documented, particularly with their application to both human gene therapy and plant biotechnology. Despite the simplicity of this approach, this technique usually ...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/g97-802
更新日期:1997-12-01 00:00:00
abstract::Polycelis nigra is a free-living simultaneous hermaphroditic flatworm that has amphimictic and pseudogamous parthenogenetic biotypes. Sexual individuals are always diploid (2n = 16) and pseudogamous parthenogens are polyploid (usually triploid). Two types of supernumerary chromosomes are found in parthenogens, those r...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-810
更新日期:1997-12-01 00:00:00
abstract::DNA gel-blot and in situ hybridization with genome-specific repeated sequences have proven to be valuable tools in analyzing genome structure and relationships in species with complex allopolyploid genomes such as hexaploid oat (Avena sativa L., 2n = 6x = 42; AACCDD genome). In this report, we describe a systematic ap...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g01-148
更新日期:2002-04-01 00:00:00
abstract::In combination with oligonucleotides of arbitrary sequence, 5' anchored oligonucleotides based on simple sequence repeats were used in polymerase chain reaction amplifications to produce barley DNA fingerprints. The aim of this work was (i) to develop a simple nonradioactive experimental procedure to reveal polymorphi...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-015
更新日期:1996-02-01 00:00:00
abstract::Five nonallelic copies of the dispersed (GATA)n repeated sequence of Drosophila melanogaster (referred to as GATA elements) have been sequenced and analysed. The GATA elements range in size from 111 to 444 bp, consisting predominantly of tandemly repeated GATAs, interspersed with variants of the subunit. The types and...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g90-113
更新日期:1990-10-01 00:00:00
abstract::We have used a PCR and cloning strategy to identify Ty1-copia-like retrotransposons in tomato, Lycopersicon esculentum Mill. Using degenerate oligonucleotide primers corresponding to conserved domains of the Ty1-copia retrotransposon reverse transcriptase (RT), fragments of about 260 bp were obtained by PCR amplificat...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g00-056
更新日期:2000-10-01 00:00:00
abstract::Post-glacial origins of woodland caribou (Rangifer tarandus subsp.) on the island of Newfoundland and their relationship to mainland populations have been uncertain. Sequence analysis of 2223 bp of the mitochondrial DNA control region and cytochrome b gene from 233 Newfoundland caribou identified 32 haplotypes in four...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0199
更新日期:2018-08-01 00:00:00
abstract::We have used immunocytochemistry and molecular cloning methods to identify and characterize structural polypeptides of the centromere. These studies permit us to resolve two distinct regions: the inner and outer centromere. (i) Components of the outer centromere: autoantibodies from certain patients with rheumatic dis...
journal_title:Genome
pub_type: 杂志文章,评审
doi:10.1139/g89-103
更新日期:1989-01-01 00:00:00
abstract::Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous underutilized legume that has the potential to improve food security in semi-arid Africa. So far, there are a lack of reports of controlled breeding populations that could be used for variety development and genetic studies. We report here the construc...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0153
更新日期:2016-07-01 00:00:00
abstract::Our goal was to purify and characterize the allelic variants H1b1 and H1b2 of histone H1.b, one of the seven subtypes of this linker histone extracted from Japanese quail erythrocyte nuclei. These variants are revealed phenotypically as band H1.3 or part of band H1.4 by polyacrylamide gel electrophoresis (PAGE) in sod...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::Chamaecyparis nootkatensis is an ecologically and economically important conifer of the north Pacific coastal forests. To aid in studies of clonal structure and genetic differentiation of this and related species, we isolated and characterized microsatellites from C. nootkatensis. A microsatellite-enriched library yie...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-014
更新日期:2003-06-01 00:00:00
abstract::Genetic diversity at 35 allozyme loci was surveyed in seven populations of Lacerta armeniaca. Fixed heterozygotes were present at 16 loci, with homozygotes at 17 loci. Variation occurred at two loci, one in each of two populations, indicating one widespread clone, one restricted clone, and one apparently restricted cl...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-141
更新日期:1995-12-01 00:00:00
abstract::Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with 4 genotypes of known Vrn genes, using previ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-028
更新日期:2007-05-01 00:00:00
abstract::The emergence of next generation sequencing has increased by several orders of magnitude the amount of data available for phylogenetics. Reduced representation approaches, such as restriction-sited associated DNA sequencing (RADseq), have proven useful for phylogenetic studies of non-model species at a wide range of p...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2016-0202
更新日期:2017-09-01 00:00:00
abstract::The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines wa...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Lycopersicon esculentum (tomato) has a small genome (2C = 1.90 pg of DNA) packaged in 2n = 2x = 24 small acrocentric to metacentric chromosomes. Like the chromosomes of other members of the family Solanaceae, tomato chromosomes have pericentromeric heterochromatin. To determine the fraction of the tomato genome found ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-011
更新日期:1996-02-01 00:00:00
abstract::Both meiosis-specific and general recombination functions, recruited from the mitotic cell cycle, are required for elevated levels of recombination and for chromosome synapsis (assembly of the synaptonemal complex) during yeast meiosis. The meiosis-specific SPO11 gene (previously shown to be required for meiotic recom...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g89-017
更新日期:1989-01-01 00:00:00
abstract::The ancestor of barley (Hordeum vulgare subsp. spontaneum) may be a source of novel alleles for crop improvement. We developed a set of recombinant chromosome substitution lines (RCSLs) using an accession of H. vulgare subsp. spontaneum (Caesarea 26-24, from Israel) as the donor and Hordeum vulgare subsp. vulgare 'Har...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g03-080
更新日期:2003-12-01 00:00:00
abstract::The isolation, physical, and genetic mapping of a group of wheat genes expressed in infected heads of Triticum aestivum 'Frontana' resistant to Fusarium head blight is reported. A cDNA library was built from heads of 'Frontana' through suppressive subtractive hybridization, to enrich for sequences induced by the patho...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g04-098
更新日期:2005-02-01 00:00:00
abstract::Quantitative trait locus (QTL) analysis was used to study the evolution of genes controlling the timing of flowering in four Brassica genomes that are all extensively replicated. Comparative mapping showed that a chromosomal region from the top of Arabidopsis thaliana chromosome 5 corresponded to three homoeologous co...
journal_title:Genome
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
abstract::Alien cytoplasms cause a wide range of phenotypic alterations in the nucleus-cytoplasm (NC) hybrids in the Triticeae. Nuclear genomes of timopheevii wheat (Triticum timopheevii and Triticum araraticum) are fully compatible with the cytoplasm of Aegilops squarrosa, while those of a majority of emmer or durum wheat cult...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g97-029
更新日期:1997-04-01 00:00:00
abstract::Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. In the present study, 3 cDNA libraries generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genom...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g07-007
更新日期:2007-03-01 00:00:00
abstract::All mRNAs on the yeast mitochondrial genome terminate at a conserved dodecamer sequence 5'-AAUAAUAUUCUU-3'. We have characterized two mutants with altered dodecamers. One contains a deletion of the dodecamer at the end of the var1 gene, and the other contains two adjacent transversions in the dodecamer at the end of t...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g89-134
更新日期:1989-01-01 00:00:00
abstract::We sequenced the complete mitochondrial (mt) genome of Camponotus atrox (Hymenoptera: Formicidae), which is only distributed in Korea. The genome was 16 540 bp in size and contained typical sets of genes (13 protein-coding genes, 22 tRNAs, and 2 rRNAs). The C. atrox A+T-rich region, at 1402 bp, was the longest of all ...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2015-0080
更新日期:2016-01-01 00:00:00
abstract::Quantitative trait loci (QTL) controlling traits associated with winterhardiness in barley (field survival, LT50, growth habit, and crown fructan content) were mapped to chromosome 7 in a population of 100 F1-derived doubled haploid lines. The largest QTL effects for all traits were detected in a 21% recombination int...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g93-009
更新日期:1993-02-01 00:00:00
abstract::The NADH-specific and NAD(P)H-bispecific nitrate reductase genes from barley have been cloned and sequenced. To determine if the Nar7 locus encodes the NAD(P)H-bispecific nitrate reductase structural gene, a cross was made between a wild-type cultivar, Morex (Nar7 Nar7), and Az70 (nar7w nar7w), a mutant from the culti...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g95-093
更新日期:1995-08-01 00:00:00
abstract::Distribution, organization, and molecular analysis of four unrelated satellite DNA components in Aveneae species are described. Highly repeated DNA elements were cloned from Helictotrichon convolutum (CON1 and CON2) and Helictotrichon compressum (COM1 and COM2). The lengths of the repeat monomers are 365 bp (CON1), 56...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g96-131
更新日期:1996-12-01 00:00:00
abstract::In an initial investigation of differential expression of genes caused by cis-acting regulatory elements in rice, the lack of reproducibility led us to question the basic premise of allelic expression imbalance determination: namely that departures of cDNA expression ratios from those observed in genomic DNA provide u...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/g2012-023
更新日期:2012-05-01 00:00:00
abstract::Knowledge of the locations of repeat elements could be very important in the assembly of genome sequences and their assignment to physical chromosomes. Genomic and species relationships among 16 species were investigated using fluorescence in situ hybridization (FISH) with the Am1 and (ACT)6 probes. The Am1 oligonucle...
journal_title:Genome
pub_type: 杂志文章
doi:10.1139/gen-2017-0132
更新日期:2018-01-01 00:00:00