Abstract:
:Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. In the present study, 3 cDNA libraries generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genomic microsatellite-enriched library, were analyzed for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs (named EST-SSR), whereas 714 genomic sequences contained 471 SSRs (named genomic-SSR). A subset of 80 SSRs, 40 EST-SSRs, and 40 genomic-SSRs were evaluated for molecular polymorphism in 23 genotypes of cultivated beans from the Mesoamerican and Andean genetic pools, including Brazilian cultivars and 2 related species. Of the common bean genotypes, 31 EST-SSR loci were polymorphic, yielding 2-12 alleles as compared with 26 polymorphic genomic-SSRs, accounting for 2-7 alleles. Cluster analysis from data using both genic and genomic-SSR revealed a clear separation between Andean and Mesoamerican beans. The usefulness of these loci for distinguishing bean genotypes and genetic mapping is discussed.
journal_name
Genomejournal_title
Genomeauthors
Hanai LR,de Campos T,Camargo LE,Benchimol LL,de Souza AP,Melotto M,Carbonell SA,Chioratto AF,Consoli L,Formighieri EF,Siqueira MV,Tsai SM,Vieira MLdoi
10.1139/g07-007subject
Has Abstractpub_date
2007-03-01 00:00:00pages
266-77issue
3eissn
0831-2796issn
1480-3321pii
g07-007journal_volume
50pub_type
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