Abstract:
:The RFLP locus Xglk605 identified by the probe Tag605 maps to a proximal position on the long arm of wheat chromosome 2B about 7 cM away from a gene conditioning resistance to cereal cyst nematode in the wheat line AUS10894. The clone Tag605 was partially sequenced and the PCR primer set AWP1 was designed. The 292-bp product, which showed no polymorphism between varieties, was cloned and sequenced. A single base difference was found in the sequence of the AWP1 products amplified and cloned from the wheat lines AUS10894 and "Spear'. PCR primers were designed with 3' termini that corresponded to the two alleles. A dual-PCR system was developed in which the primer sets AWP2 and AWP3 produced allele-specific amplification. The concentration of the oligonucleotide primers and the sequence of the primer-template mismatches were critical to the success of discriminatory allele amplification.
journal_name
Genomejournal_title
Genomeauthors
Williams KJ,Fisher JM,Langridge Pdoi
10.1139/g96-100subject
Has Abstractpub_date
1996-08-01 00:00:00pages
798-801issue
4eissn
0831-2796issn
1480-3321journal_volume
39pub_type
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