Development of species-specific primers with potential for amplifying eDNA from imperilled freshwater unionid mussels.

abstract：:A previous study of 100 karyotyped metaphase cells has demonstrated the utility of a graphic arts tool in deriving chromosome measurements for relative length determination. In the present study we utilize this same approach to address the question: "What are the average differences in relative lengths between apparen...

journal_title：Genome

authors： Mark HF,Wyandt HE,Pan T,Mark R Jr,Paramenter M,Campbell W,Mark Y

更新日期：1993-10-01 00:00:00

abstract：:The genomic content of the subtelomeric repeated sequences Spelt1 and Spelt52 was studied by dot, Southern, and in situ hybridization in 11 newly synthesized amphiploids of Aegilops and Triticum, and data were compared with the parental plants. Spelt1 had reduced copy numbers in the first generation of three synthetic...

journal_title：Genome

authors： Salina EA,Numerova OM,Ozkan H,Feldman M

更新日期：2004-10-01 00:00:00

abstract：:A recent investigation, using a human genomic probe, has indicated that the 68,000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p less than 0.0001). In addition, we have fou...

journal_title：Genome

authors： Somerville MJ,McLachlan DR,Percy ME

更新日期：1988-08-01 00:00:00

abstract：:Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) possesses a number of valuable genes against biotic and abiotic stress, which could be transferred into common wheat background for wheat improvement. In the present study, we determined the karyotypic constitution of a wheat - L. mollis double disomic addition line...

journal_title：Genome

authors： Zhang A,Li W,Wang C,Yang X,Chen C,Zhu C,Peng N,Tian Z,Wang Y,Zhang H,Liu X,Ji W

更新日期：2017-05-01 00:00:00

abstract：:Mapping and sequencing the human genome will generate large amounts of data, which must be sorted, analyzed, and stored for rapid retrieval to complete this enormous task. Computers and their software programs provide the most important tool to the molecular biologist today. A discussion of current capabilities and fu...

journal_title：Genome

authors： Kelly MJ

更新日期：1989-01-01 00:00:00

abstract：:The first doubled haploid oat linkage map constructed at MTT Agrifood Research Finland was supplemented with additional microsatellites and Diversity Array Technology (DArT) markers to produce a map containing 1058 DNA markers and 34 linkage groups. The map was used to locate quantitative trait loci (QTLs) for 11 impo...

journal_title：Genome

authors： Tanhuanpää P,Manninen O,Beattie A,Eckstein P,Scoles G,Rossnagel B,Kiviharju E

更新日期：2012-04-01 00:00:00

abstract：:Aegilops is a genus belonging to the family Poaceace, which have played an indispensible role in the evolution of bread wheat and continues to do so by transferring genes by wide hybridization. Being the secondary gene pool of wheat, gene transfer from Aegilops poses difficulties and segregation distortion is common. ...

journal_title：Genome

authors： Niranjana M

更新日期：2017-08-01 00:00:00

abstract：:The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines wa...

journal_title：Genome

authors： Crockett PA,Bhalla PL,Lee CK,Singh MB

更新日期：2000-04-01 00:00:00

abstract：:A reminder that another paper on the cetacean PRNP locus has been published before Kim et al.'s paper (2008. Genome, 51: 452-464) is presented along with a consideration of the related results. ...

journal_title：Genome

authors： Acutis PL,Peletto S,Grego E,Colussi S,Riina MV,Rosati S,Mignone W,Caramelli M

更新日期：2008-12-01 00:00:00

abstract：:5S rDNA loci have been mapped on barley chromosomes by in situ hybridization using five reciprocal translocation lines. Two kinds of DNA probes covering either the 5S rDNA coding region or the 5S rDNA coding and flanking noncoding regions were used. They were prepared by direct cloning from interphase nuclei and simul...

journal_title：Genome

authors： Fukui K,Kamisugi Y,Sakai F

更新日期：1994-02-01 00:00:00

abstract：:Hamster beta-tubulin (detected as a mutant subunit that confers Colcemid resistance) is either not expressed or is underexpressed in Chinese hamster-mouse somatic cell hybrids. This selectivity of tubulin expression suggests that a uniparental mouse spindle might preferentially engage mouse chromosomes and lead to los...

journal_title：Genome

authors： Zelesco PA,Graves JA

更新日期：1987-08-01 00:00:00

abstract：:The combination of two divergent genomes during hybridization can result in "genome shock". Although genome shock has been reported in the hybrids of some herbaceous plants, the pattern and the principle it follows are far from understood, especially in woody plants. Here, the gene expression patterns were remodeled i...

journal_title：Genome

authors： Zhang M,Liu XK,Fan W,Yan DF,Zhong NS,Gao JY,Zhang WJ

更新日期：2018-07-01 00:00:00

abstract：:The isolation, physical, and genetic mapping of a group of wheat genes expressed in infected heads of Triticum aestivum 'Frontana' resistant to Fusarium head blight is reported. A cDNA library was built from heads of 'Frontana' through suppressive subtractive hybridization, to enrich for sequences induced by the patho...

journal_title：Genome

authors： Han FP,Fedak G,Ouellet T,Dan H,Somers DJ

更新日期：2005-02-01 00:00:00

abstract：:Three wheat sequences, shown to be homologous to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes were localized on chromosomes using nullisomic-tetrasomic wheat ('Chinese Spring') and wheat-rye ('Chinese Spring'/'Imperial') addition lines. Whereas the loci for the pol...

journal_title：Genome

authors： Korzun V,Balzer HJ,Balzer A,Bäumlein H,Börner A

更新日期：1996-12-01 00:00:00

abstract：:Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea L. (2n = 4x = 40) and A. cardenasii Krapov. & W. C. Gregory (2n = 2x = 20) were analyzed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-speci...

journal_title：Genome

authors： Garcia GM,Stalker HT,Kochert G

更新日期：1995-02-01 00:00:00

abstract：:The complete sequence (15,455 bp) of the mitochondrial DNA of the mosquito Anopheles quadrimaculatus species A is reported. This genome is compact and very A+T rich (77.4% A+T). It contains genes for 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and 13 subunits of the mitochondrial inner membrane respiratory com...

journal_title：Genome

authors： Mitchell SE,Cockburn AF,Seawright JA

更新日期：1993-12-01 00:00:00

abstract：:Genotyping-by-sequencing (GBS) is a rapid, flexible, low-cost, and robust genotyping method that simultaneously discovers variants and calls genotypes within a broad range of samples. These characteristics make GBS an excellent tool for many applications and research questions from conservation biology to functional g...

journal_title：Genome

authors： Torkamaneh D,Laroche J,Belzile F

更新日期：2020-11-01 00:00:00

abstract：:The morphological picture of primate phylogeny has not unambiguously identified the nearest outgroup of Anthropoidea and has not resolved the branching pattern within Hominoidea. The molecular picture provides more resolution and clarifies the systematics of Hominoidea. Protein and DNA evidence divides Hominoidea into...

journal_title：Genome

authors： Goodman M,Koop BF,Czelusniak J,Fitch DH,Tagle DA,Slightom JL

更新日期：1989-01-01 00:00:00

abstract：:Photographic maps and rearrangements of each salivary gland polytene chromosome arm of Anopheles nemophilous (species F) and of An. dirus species A, B, C, and D of the Dirus group from natural populations in Thailand are presented. Structural conformation of heterokaryotypes and comparison of chromosome banding sequen...

journal_title：Genome

authors： Poopittayasataporn A,Baimai V

更新日期：1995-06-01 00:00:00

abstract：:This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of...

journal_title：Genome

authors： Yowe DL,Epping RJ

更新日期：1996-10-01 00:00:00

abstract：:Numerous quantitative trait loci (QTL) have been detected in Brassica species, but fine-mapping of major QTL has advanced slowly. The development of functional markers can overcome this barrier. We used publicly available PlantGDB-assembled unique transcripts (PUTs) from Brassica species to design 7836 functional simp...

journal_title：Genome

authors： An Z,Gao C,Li J,Fu D,Tang Z,Ortegón O

更新日期：2011-09-01 00:00:00

abstract：:A model for the genetic structure of grape phylloxera populations in Europe was developed using hierarchical sampling techniques and AFLP-PCR (amplified fragment length polymorphism--polymerase chain reaction) methodology. One-hundred three European and 6 North American phylloxera populations were studied. An addition...

journal_title：Genome

authors： Forneck A,Walker MA,Blaich R

更新日期：2000-08-01 00:00:00

abstract：:In an effort to expand the Gossypium hirsutum L. (cotton) expressed sequence tag (EST) database, ESTs representing a variety of tissues and treatments were sequenced. Assembly of these sequences with ESTs already in the EST database (dbEST, GenBank) identified 9675 cotton sequences not present in GenBank. Statistical ...

journal_title：Genome

authors： Taliercio E,Allen RD,Essenberg M,Klueva N,Nguyen H,Patil MA,Payton P,Millena AC,Phillips AL,Pierce ML,Scheffler B,Turley R,Wang J,Zhang D,Scheffler J

更新日期：2006-04-01 00:00:00

abstract：:Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify ...

journal_title：Genome

authors： Holland JB,Helland SJ,Sharopova N,Rhyne DC

更新日期：2001-12-01 00:00:00

abstract：:Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obta...

journal_title：Genome

authors： Pedersen C,Langridge P

更新日期：1997-10-01 00:00:00

abstract：:The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspec...

journal_title：Genome

authors： Besnard G,El Bakkali A

更新日期：2014-03-01 00:00:00

abstract：:European hazelnut (Corylus avellana L.) is the only economically important nut crop in the family Betulaceae. Because of its small genome size (~385 Mb / 1C), relatively short life cycle, availability of a dense linkage map, and amenability to transformation by Agrobacterium, the European hazelnut could serve as a mod...

journal_title：Genome

authors： Sathuvalli VR,Mehlenbacher SA

更新日期：2011-10-01 00:00:00

abstract：:Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We hav...

journal_title：Genome

authors： Kang ST,Mian MA

更新日期：2010-05-01 00:00:00

abstract：:Differences in genome size and gene content are among the most important signatures of microbial adaptation and genome evolution. Here, we investigated the patterns of genome variation among 10 natural strains of the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti, using pulse field gel electrophoresis (PFG...

journal_title：Genome

authors： Guo H,Sun S,Eardly B,Finan T,Xu J

更新日期：2009-10-01 00:00:00

abstract：:This paper describes a series of winter wheat - winter barley disomic addition lines developed from hybrids between winter wheat line Triticum aestivum L. 'Martonvásári 9 kr1' and the German 2-rowed winter barley cultivar Hordeum vulgare L. 'Igri'. The barley chromosomes in a wheat background were identified from the ...

journal_title：Genome

authors： Szakács E,Molnár-Láng M

更新日期：2007-01-01 00:00:00