Alterations in subtelomeric tandem repeats during early stages of allopolyploidy in wheat.

Abstract:

:The genomic content of the subtelomeric repeated sequences Spelt1 and Spelt52 was studied by dot, Southern, and in situ hybridization in 11 newly synthesized amphiploids of Aegilops and Triticum, and data were compared with the parental plants. Spelt1 had reduced copy numbers in the first generation of three synthetic amphiploids, but two others did not change; Spelt52 was amplified in nine amphiploids and did not change in two. In the second allopolyploid generation, Spelt1 copy number did not change, whereas there was amplification of Spelt52 in some allopolyploids and decreases in others. Neither allopolyploidy level nor the direction of the cross affected the patterns of change in the newly synthesized amphiploids. Changes did not result from intergenomic recombination because similar alterations were noticed in allopolyploids with and without Ph1, a gene that suppresses homoeologous pairing. No differences in Spelt1 and Spelt52 tandem organization were found by Southern hybridization. The significance of these data are discussed in relation to the establishment of newly formed allopolyploids.

journal_name

Genome

journal_title

Genome

authors

Salina EA,Numerova OM,Ozkan H,Feldman M

doi

10.1139/g04-044

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

860-7

issue

5

eissn

0831-2796

issn

1480-3321

pii

g04-044

journal_volume

47

pub_type

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