Abstract:
:In an effort to expand the Gossypium hirsutum L. (cotton) expressed sequence tag (EST) database, ESTs representing a variety of tissues and treatments were sequenced. Assembly of these sequences with ESTs already in the EST database (dbEST, GenBank) identified 9675 cotton sequences not present in GenBank. Statistical analysis of a subset of these ESTs identified genes likely differentially expressed in stems, cotyledons, and drought-stressed tissues. Annotation of the differentially expressed cDNAs tentatively identified genes involved in lignin metabolism, starch biosynthesis and stress response, consistent with pathways likely to be active in the tissues under investigation. Simple sequence repeats (SSRs) were identified among these ESTs, and an inexpensive method was developed to screen genomic DNA for the presence of these SSRs. At least 69 SSRs potentially useful in mapping were identified. Selected amplified SSRs were isolated and sequenced. The sequences corresponded to the EST containing the SSRs, confirming that these SSRs will potentially map the gene represented by the EST. The ESTs containing SSRs were annotated to help identify the genes that may be mapped using these markers.
journal_name
Genomejournal_title
Genomeauthors
Taliercio E,Allen RD,Essenberg M,Klueva N,Nguyen H,Patil MA,Payton P,Millena AC,Phillips AL,Pierce ML,Scheffler B,Turley R,Wang J,Zhang D,Scheffler Jdoi
10.1139/g05-115subject
Has Abstractpub_date
2006-04-01 00:00:00pages
306-19issue
4eissn
0831-2796issn
1480-3321pii
g05-115journal_volume
49pub_type
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